Enhanced somatic embryogenesis, plant regeneration and total phenolic content estimation in Lycium barbarum L.: a highly nutritive and medicinal plant

Verma, Sandeep Kumar; Gantait, Saikat; Mukherjee, Epsita; Gürel, Ekrem

doi:10.1007/s12892-022-00150-8

Cited by 7 publications

(7 citation statements)

References 37 publications

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“…The role of Picloram on somatic embryogenesis has been studied for the first time and identified as an effective auxin in L. barbarum ( Table 3 ). Typically, 2,4-D has been the most used auxin for somatic embryogenesis [ 22 , 24 ]. Induction of somatic embryos on a medium containing Picloram has been reported in strawberry ( Fragaria ananassa L.) [ 29 ].…”

Section: Resultsmentioning

confidence: 99%

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Effect of Picloram and Desiccation on the Somatic Embryogenesis of Lycium barbarum L.

Khatri,

Joshee

2024

Plants

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An efficient and reproducible in vitro method for indirect somatic embryogenesis was optimized by culturing leaf and leaf with petiole explants of Lycium barbarum L. Murashige and Skoog (MS) medium, supplemented with various concentrations of Picloram and 2,4-Dichlorophenoxyacetic acid (2,4-D), individually and in combinations, were tested. Picloram (1.0 µM) showed a better response compared to 2,4-D and results indicate it to be a better auxin for induction of somatic embryos for Goji berry. It was seen that the leaf explants were more responsive in callus and somatic embryo induction than the leaf with petiole explant when incubated in the dark for 5 weeks. Embryogenic callus, after being transferred to MS medium containing Benzyl amino purine (BAP) in 1.0 µM, 5.0 µM and 10.0 µM, began to differentiate in light after one week. MS medium with 1.0 µM Picloram + 10 µM BAP resulted as the most favorable treatment for somatic embryogenesis in Lycium barbarum L. Removal of plant growth regulators from MS medium and culturing induced calluses under 16 h photoperiod resulted in globular, heart, torpedo, cotyledons, and further development into plantlets. Well-developed plants have been obtained and are capable of acclimatizing in ex vitro conditions. In addition, the effects of desiccation treatments (0, 1, 3, 6, 9 h, and 12 h) on embryogenic callus for somatic embryo induction were found to be directly proportional to the length of desiccation treatment at room temperature. After 9 h and 12 h of desiccation treatments, 60% and 90% of plated calluses resulted in somatic embryos, respectively. In a L. barbarum callus mass, Acetocarmine and Evans blue double staining differentiated between embryogenic and non-embryogenic callus. These findings will help Goji berry improvement by elite clone production, ex situ conservation projects, scaling up plant production, and agronomy for the commercial production of this superfruit in the future.

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Section: Resultsmentioning

confidence: 99%

“…In addition, it was stated that callus induction was two times more efficient under Picloram as compared to 2,4-D [ 30 ]. Osman et al [ 22 ] and Verma et al [ 24 ] achieved somatic embryogenesis in Goji berry with the supplementation of 2,4-D alone.…”

Section: Resultsmentioning

confidence: 99%

Effect of Picloram and Desiccation on the Somatic Embryogenesis of Lycium barbarum L.

Khatri,

Joshee

2024

Plants

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“…The adventitious shoot regeneration technique has been successfully employed for mass propagation of berry crops using semisolid (Qiu et al, 2018;Schuchovski et al, 2020) and liquid media in bioreactors (Debnath, 2014;Arigundam et al, 2020). While working with blackberry (Rubus fruticosus), blueberry (V. corymbosum), and kiwiberry (Actinidia argute), Hunkováet al (2022) regenerated shoots from petioles and internodal segments; Verma et al (2022) reported plant regeneration through adventitious shoot formation from hypocotyl and leaf explants of Lycium barbarum (Goji), on a semisolid medium.…”

Section: In Vitro Propagationmentioning

confidence: 99%

“…While working with blackberry ( Rubus fruticosus ), blueberry ( V. corymbosum ), and kiwiberry ( Actinidia argute ), Hunková et al. (2022) regenerated shoots from petioles and internodal segments; Verma et al. (2022) reported plant regeneration through adventitious shoot formation from hypocotyl and leaf explants of Lycium barbarum (Goji), on a semisolid medium.…”

Section: In Vitro Propagationmentioning

confidence: 99%

“…There are few available detailed reports on SE in blueberries ( Ghosh et al., 2017 ; Ghosh et al., 2018 ). However, studies are available on SE in grapes ( Nakajima and Matsuta, 2003 ; Dhekney et al, 2016 ; Forleo et al., 2021 ), strawberry ( Biswas et al., 2009 ), Arbutus unedo (strawberry tree; Martins et al, 2022 ) and L. barbarum ( Verma et al., 2022 ) on semisolid media. Reports on SE in berry crops are scarce in a bioreactor system.…”

Section: In Vitro Propagationmentioning

confidence: 99%

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Phenotypic variation and epigenetic insight into tissue culture berry crops

Debnath

Ghosh

2022

Front. Plant Sci.

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Berry crops, a nutrient powerhouse for antioxidant properties, have long been enjoyed as a health-promoting delicious food. Significant progress has been achieved for the propagation of berry crops using tissue culture techniques. Although bioreactor micropropagation has been developed as a cost-effective propagation technology for berry crops, genetic stability can be a problem for commercial micropropagation that can be monitored at morphological, biochemical, and molecular levels. Somaclonal variations, both genetic and epigenetic, in tissue culture regenerants are influenced by different factors, such as donor genotype, explant type and origin, chimeral tissues, culture media type, concentration and combination of plant growth regulators, and culture conditions and period. Tissue culture regenerants in berry crops show increased vegetative growth, rhizome production, and berry yield, containing higher antioxidant activity in fruits and leaves that might be due to epigenetic variation. The present review provides an in-depth study on various aspects of phenotypic variation in micropropagated berry plants and the epigenetic effects on these variations along with the role of DNA methylation, to fill the existing gap in literature.

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Biotechnological Tools for Conservation and Sustainable Utilization of Medicinal Plants

Kumar,

Sharma,

Kumar

2024

Role of Science and Technology for Sustainable Future

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Enhanced somatic embryogenesis, plant regeneration and total phenolic content estimation in Lycium barbarum L.: a highly nutritive and medicinal plant

Cited by 7 publications

References 37 publications

Effect of Picloram and Desiccation on the Somatic Embryogenesis of Lycium barbarum L.

Effect of Picloram and Desiccation on the Somatic Embryogenesis of Lycium barbarum L.

Phenotypic variation and epigenetic insight into tissue culture berry crops

Biotechnological Tools for Conservation and Sustainable Utilization of Medicinal Plants

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