“…Biolistic gene transfer can be applied to a wider range of genotypes than Agrobacterium-mediated gene transfer (Altpeter et al, 2005) but can be limited by the inability to regenerate plants after bombardment. Regeneration response and transgene performance following biolistic gene transfer depend on particle type, size, quantity and acceleration, DNA amount and structure during particle coating, tissue type, and pretreatment (Klein et al, 1988;Vain et al, 1993;Kausch et al, 1995;Frame et al, 2000;Fu et al, 2000;Popelka et al, 2003;Sandhu and Altpeter 2008;Lowe et al, 2009;Sivamani et al, 2009;Wu et al, 2015). Therefore, there is a need for the development of alternative nano-or microparticles, target tissues, and particle coating and delivery protocols for biolistic gene transfer.…”