Enhanced sialylation of mucin-associated carbohydrate structures in human colon cancer metastasis

Bresalier, Robert S.; Ho, Samuel B.; Schoeppner, H; Kim, Young S.; Sleisenger, Marvin H.; Brodt, Pnina; Byrd, James C.

doi:10.1053/gast.1996.v110.pm8613039

Cited by 158 publications

(115 citation statements)

References 15 publications

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“…The incidence of liver recurrence was significantly higher in SLXpositive patients than in SLX-negative ones (P = 0.028). These results were consistent with the results of previous animal and in vitro reports, which stated that SLX was a ligand carbohydrate of selection and played an important role during haematogenous metastasis (Saitoh et al, 1992;Sawada et al, 1992;Takada et al, 1993;Izumi et al, 1995;Bresalier et al, 1996). The present study revealed that the frequencies of SLX positivity, synchronous liver metastasis and liver recurrence were significantly higher with differentiated carcinoma than with undifferentiated carcinoma (P < 0.0001, P = 0.021 and P = 0.028, respectively).…”

Section: Discussionsupporting

confidence: 93%

“…In vitro study showed that these carbohydrates were involved in the adhesion of tumour cells from gastric carcinoma, colon carcinoma, hepatoma, pancreatic carcinoma and lung carcinoma to E-selectin, which is present on cytokine-activated human endothelial cells (Takada et al, 1993). It was also reported that colon carcinoma cells which were highly metastatic to the liver expressed relatively more SLX structures than their corresponding low metastatic counterparts, and that the increased expression of SLX correlated with strong adherence to vascular endothelial cells with E-selectin (Saitoh et al, 1992;Sawada et al, 1992;Izumi et al, 1995;Bresalier et al, 1996). These findings suggest that SLX play important roles during haematogeneous tumour metastasis.…”

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confidence: 94%

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Clinicopathologic significance of sialyl Le xexpression in advanced gastric carcinoma

et al. 2000

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SummarySialyl Lewis x antigen (SLX) is a carbohydrate antigen that serves as a ligand for selectin, an adhesion molecule expressed on vascular endothelial cells. The expression of SLX in 245 patients with advanced gastric carcinoma was examined immunohistochemically, and its clinicopathologic significance was analysed. We classified the patients with advanced gastric carcinoma into 91 with differentiated type and 154 with undifferentiated type. SLX expressed in 135 of 245 patients (55%), comprising 68 (75%) patients with differentiated carcinoma and 67 (44%) with undifferentiated carcinoma. The positive rate for SLX expression was significantly higher among patients with differentiated carcinoma than among those in undifferentiated carcinoma (P < 0.0001). With differentiated carcinoma, the incidence of lymph node metastasis, advanced tumour stage (stage III and IV) and liver recurrence was significantly higher in SLX-positive patients than in SLXnegative ones (P < 0.0001, P = 0.0065 and P = 0.028, respectively). Moreover, the prognoses were better in patients with SLX-negative tumours than in those with SLX-positive tumours (P = 0.019). With undifferentiated carcinoma, there were no significant correlations between SLX expression and any clinicopathological features or prognoses. The clinicopathologic significance of SLX expression in gastric carcinoma patients depends on histologic type. SLX expression may be of great relevance in predicting liver metastases in patients with differentiated carcinoma.

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Section: Discussionsupporting

confidence: 93%

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confidence: 94%

Clinicopathologic significance of sialyl Le xexpression in advanced gastric carcinoma

et al. 2000

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“…Detection of mucins during Raman analysis therefore provides an additional scope for assessing tissue homoeostasis and early metastatic potential (Bresalier et al . 1996; Kufe 2009; Mekenkamp et al . 2012).…”

Section: Discussionmentioning

confidence: 99%

Label‐free Raman spectroscopic imaging to extract morphological and chemical information from a formalin‐fixed, paraffin‐embedded rat colon tissue section

Gaifulina

Maher

Kendall

et al. 2016

Int J Experimental Path

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SummaryAnimal models and archived human biobank tissues are useful resources for research in disease development, diagnostics and therapeutics. For the preservation of microscopic anatomical features and to facilitate long‐term storage, a majority of tissue samples are denatured by the chemical treatments required for fixation, paraffin embedding and subsequent deparaffinization. These aggressive chemical processes are thought to modify the biochemical composition of the sample and potentially compromise reliable spectroscopic examination useful for the diagnosis or biomarking. As a result, spectroscopy is often conducted on fresh/frozen samples. In this study, we provide an extensive characterization of the biochemical signals remaining in processed samples (formalin fixation and paraffin embedding, FFPE) and especially those originating from the anatomical layers of a healthy rat colon. The application of chemometric analytical methods (unsupervised and supervised) was shown to eliminate the need for tissue staining and easily revealed microscopic features consistent with goblet cells and the dense populations of cells within the mucosa, principally via strong nucleic acid signals. We were also able to identify the collagenous submucosa‐ and serosa‐ as well as the muscle‐associated signals from the muscular regions and blood vessels. Applying linear regression analysis to the data, we were able to corroborate this initial assignment of cell and tissue types by confirming the biological origin of each layer by reference to a subset of authentic biomolecular standards. Our results demonstrate the potential of using label‐free Raman microspectroscopy to obtain superior imaging contrast in FFPE sections when compared directly to conventional haematoxylin and eosin (H&E) staining.

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“…Several studies have shown a relationship between loss of cadherin or integrin molecules and acquisition of an invasive phenotype by tumor cells (Navarro et al, 1991). Carbohydrate structures like mucin-associated sialoglycoproteins have been used to determine prognosis in patients with colorectal cancer; increased sialylation of mucin carbohydrates is associated with a higher metastatic potential (Bresalier et al, 1996).…”

Section: Introductionmentioning

confidence: 99%

A nude mice model of human rhabdomyosarcoma lung metastases for evaluating the role of polysialic acids in the metastatic process

Daniel

Durbec²,

Gautherot³

et al. 2001

Oncogene

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PSA is an oncodevelopmental antigen usually expressed in human tumors with high metastatic potential. Here we set up a metastatic model in nude mice by using TE671 cells, which strongly express PSA-NCAM. We observed the formation of lung metastases when TE671 cells were injected intravenously, intramuscularly, and intraperitoneously, but not subcutaneously. Intraperitoneous injections also induced peritoneal carcinosis, ascites, and liver metastases. To evaluate the putative role of PSA in the metastatic process we used a speci®c cleavage of PSA on NCAM by endoneuraminidase-N on intraperitoneous primary tumors. Mice with primary intramuscular tumors were taken as control. Repeated injections of endoneuraminidase-N led to a decrease in PSA expression in primary intraperitoneous nodules and ascites but not in intramuscular primary tumors. Endoneuraminidase-N also increased the delay in ascitic formation and decreased the number of lung or liver metastases in the case of intraperitoneous tumors but not in the case of intramuscular tumors. When metastases occurred in endoneuraminidase-N injected animals, they strongly expressed PSA-NCAM. Therefore, we established a relationship between PSA expression on the surface of primary tumor cells and the metastatic process. Oncogene (2001) 20, 997 ± 1004.

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Enhanced sialylation of mucin-associated carbohydrate structures in human colon cancer metastasis

Cited by 158 publications

References 15 publications

Clinicopathologic significance of sialyl Le xexpression in advanced gastric carcinoma

Clinicopathologic significance of sialyl Le xexpression in advanced gastric carcinoma

Label‐free Raman spectroscopic imaging to extract morphological and chemical information from a formalin‐fixed, paraffin‐embedded rat colon tissue section

A nude mice model of human rhabdomyosarcoma lung metastases for evaluating the role of polysialic acids in the metastatic process

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