Enhanced separation of colloidal particles in an AsPFF device with a tilted sidewall and vertical focusing channels (t-AsPFF-v)

Nho, Hyun Woo; Yoon, Tae Hyun

doi:10.1039/c2lc41154g

Cited by 15 publications

(26 citation statements)

References 16 publications

(18 reference statements)

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“…In PFF, solutions of particles and buffer are respectively injected from two separate inlets in the chip and travel at different flow rates until reaching a pinched segment where the particles are pushed towards a side wall and they are constrained to move on streamlines defined by the positions of their centers of mass (which varies according to their size) [ 148 ]. Then, the solution moves towards a broadened segment of the device where the various streamlines (and particles within) are separated and collected from different outlets.…”

Section: Isolation Of Exosomes and Microvesicles From Biological Fmentioning

confidence: 99%

Lab-on-Chip for Exosomes and Microvesicles Detection and Characterization

Chiriaco

Bianco

Nigro

et al. 2018

Sensors

125

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Interest in extracellular vesicles and in particular microvesicles and exosomes, which are constitutively produced by cells, is on the rise for their huge potential as biomarkers in a high number of disorders and pathologies as they are considered as carriers of information among cells, as well as being responsible for the spreading of diseases. Current methods of analysis of microvesicles and exosomes do not fulfill the requirements for their in-depth investigation and the complete exploitation of their diagnostic and prognostic value. Lab-on-chip methods have the potential and capabilities to bridge this gap and the technology is mature enough to provide all the necessary steps for a completely automated analysis of extracellular vesicles in body fluids. In this paper we provide an overview of the biological role of extracellular vesicles, standard biochemical methods of analysis and their limits, and a survey of lab-on-chip methods that are able to meet the needs of a deeper exploitation of these biological entities to drive their use in common clinical practice.

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Section: Isolation Of Exosomes and Microvesicles From Biological Fmentioning

confidence: 99%

Lab-on-Chip for Exosomes and Microvesicles Detection and Characterization

Chiriaco

Bianco

Nigro

et al. 2018

Sensors

125

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“…The microchannel is composed of Sample and Function inlets, nine outlets, and a magnification channel which adjusts particle allocation by altering MR. Similar adaptation of magnification channel has been introduced 25 , 26 for vesicles/particles to move further away from each other. In our case, magnification increased the separation efficiency of vesicles/particles by draining Function flow through the magnification channel.…”

Section: Discussionmentioning

confidence: 99%

Separation of extracellular nanovesicles and apoptotic bodies from cancer cell culture broth using tunable microfluidic systems

Shin

Han

Park

et al. 2017

Sci Rep

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Extracellular vesicles (EVs) are the cell-secreted nano- and micro-sized particles consisted of lipid bilayer containing nucleic acids and proteins for diagnosis and therapeutic applications. The inherent complexity of EVs is a source of heterogeneity in various potential applications of the biological nanovesicles including analysis. To diminish heterogeneity, EV should be isolated and separated according to their sizes and cargos. However, current technologies do not meet the requirements. We showed noninvasive and precise separation of EVs based on their sizes without any recognizable damages. We separated atto-liter volumes of biological nanoparticles through operation of the present system showing relatively large volume of sample treatment to milliliters within an hour. We observed distinct size and morphological differences of 30 to 100 nm of exosomes and apoptotic bodies through TEM analysis. Indeed, we confirmed the biological moiety variations through immunoblotting with noninvasively separated EVs opening new windows in study and application of the biological nanoparticles.

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“… a An integrated DLD device with a mirrored array structure to capture CTCs 2 . b Mirrored DLD structure for microalgae cell isolation 177 . c A mirrored nanometer DLD array to concentrate extracellular vesicles 84 .…”

Section: Different Biological Micro-object Separation Microfluidic Sc...mentioning

confidence: 99%

“…c Duplicated PFF structure to amplify lateral displacement twice 151 . d PFF with parallelogram focusing channel 177 . e A serpentine pillar array design of the microfiltration device.…”

Section: Different Biological Micro-object Separation Microfluidic Sc...mentioning

confidence: 99%

“…The last modification is on a microfocusing channel (Fig. 9d ) 177 , which aims to strengthen the focusing effect and lateral separation (shape optimization). By changing the rectangular cross section of the channel of AsPFF (there is a drainage in the funnel-like channel) to a tilted one and adding a vertical focusing channel, the separation distance of particles is increased from ( r L – r S ) to ( r L – r S )/tan( θ /2).…”

Section: Different Biological Micro-object Separation Microfluidic Sc...mentioning

confidence: 99%

See 1 more Smart Citation

Geometric structure design of passive label-free microfluidic systems for biological micro-object separation

et al. 2022

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Passive and label-free microfluidic devices have no complex external accessories or detection-interfering label particles. These devices are now widely used in medical and bioresearch applications, including cell focusing and cell separation. Geometric structure plays the most essential role when designing a passive and label-free microfluidic chip. An exquisitely designed geometric structure can change particle trajectories and improve chip performance. However, the geometric design principles of passive and label-free microfluidics have not been comprehensively acknowledged. Here, we review the geometric innovations of several microfluidic schemes, including deterministic lateral displacement (DLD), inertial microfluidics (IMF), and viscoelastic microfluidics (VEM), and summarize the most creative innovations and design principles of passive and label-free microfluidics. We aim to provide a guideline for researchers who have an interest in geometric innovations of passive label-free microfluidics.

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Enhanced separation of colloidal particles in an AsPFF device with a tilted sidewall and vertical focusing channels (t-AsPFF-v)

Cited by 15 publications

References 16 publications

Lab-on-Chip for Exosomes and Microvesicles Detection and Characterization

Lab-on-Chip for Exosomes and Microvesicles Detection and Characterization

Separation of extracellular nanovesicles and apoptotic bodies from cancer cell culture broth using tunable microfluidic systems

Geometric structure design of passive label-free microfluidic systems for biological micro-object separation

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