Enhanced selectivity for capillary zone electrophoresis using ion-pair agents

Shelton, Chris M.; Koch, Jeremy T.; Desai, Nehal; Wheeler, John F.

doi:10.1016/s0021-9673(97)00924-2

Cited by 28 publications

(10 citation statements)

References 26 publications

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“…Ion-pair reagents such as tetrabuytlammonium bromide or sodium heptanesulphonate have been used in conventional CE [42] and MEKC [43,44]. The ion-pair reagents have their own mobility and can have hydrophobic interactions with solutes in a chromatographic manner [45].…”

Section: Ion-pair Reagentsmentioning

confidence: 99%

The effect of operating variables in microemulsion electrokinetic capillary chromatography

Altria¹,

2000

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SummaryMicroemulsion electrokinetic capillary chromatography (MEEKC) is similar to micellar electrokinetic chromatography (MEKC) in that it separates neutral solutes based on their chromatographic retention factors. In MEEKC solutes partition bek, veen the aqueous phase and oil droplets, which are moving through the solution. The background to MEEKC is described including novel approaches to method development and optimisation. In this case water-immiscible octane forms minute oil droplets that are coated with SDS and butan-1-ok The effects were evaluated using a test-mixture containing nine components of insoluble and soluble acids, bases and neutrals. Selectivity has been adjusted by use of a large number of factors including organic solvent, co-su rfactant, urea, temperature, cyclodextrins, ion-pair reagent. Previous reports on the selectivity in MEEKC have concentrated only on neutral solutes. Separation selectivity was drastically changed with addition of alcohol such as butan-l-ol, propan-2-ol, cyclodextrin or using a low pH buffer. Microemulsion preparation process or filtration of the buffer did not affect the separation. The separation was largely unaffected by the use of methanol or acetonitrile, surfactant concentration, buffer type, oil type, sample diluent or type of the counter-ion. Migration times were dramatically altered with the use of ion-pair reagent and buffer concentration. It was also demonstrated that temperature variations alter the migration time but not the selectivity.

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Section: Ion-pair Reagentsmentioning

confidence: 99%

The effect of operating variables in microemulsion electrokinetic capillary chromatography

Altria¹,

2000

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“…74 Alkylsulfonate ions were used for the separation of angiotensin-converting enzyme inhibitors. 75 Applications of conventional ion-pairing reagents to CZE separation are reviewed by Shelton et al, 77 and chiral separations are reviewed by Barbas and Saavedra. 78 4·2 Equilibrium analysis of ion association reactions by CZE Ion association reaction in aqueous solution is thus useful for resolution improvement in capillary zone electrophoresis.…”

Section: ·1 Utilization Of Ion Association Reactions In Cze Separationsmentioning

confidence: 99%

Analysis of Ion-Association Reaction in Aqueous Solution and Its Utilization by Capillary Zone Electrophoresis

Takayanagi

2004

ANAL. SCI.

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“…6). The chiral separation of Ru(II) complexes with a-diimine ligands, though without testing their cytotoxic activity, was addressed in systematic studies by the group of Kane-Maguire and Wheeler [55][56][57][58][59][60]. Interestingly to the subject of this review, among several optically active species incorporated into the background electrolyte (BGE) to specifically interact with the migrating isomers, B-form of DNA was shown feasible as the chiral resolving agent [55,57,58].…”

Section: Chiral Separationsmentioning

confidence: 99%

Capillary electrophoresis in anti‐cancer metallodrug research: Advances and future challenges

2003

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An efficient and convenient separation method has been a long sought after goal for anti-cancer metallodrug developers. For many reasons, capillary electrophoresis (CE) has recently emerged as the method of choice for the separation of intact platinum metal complexes and their metabolites, assessment of drug stability, and studying the interaction of the administered and potential tumor-inhibiting metallocomplexes with biomolecules. Due to the application of gentle separation conditions and successful developments in combinations with molecule-specific detectors, CE is also growing in importance as a versatile tool for the characterization of specific metal-bioligand binding products and thereby for providing mechanism-of-action information. Recent advances in metallodrug monitoring by CE are reviewed and critically evaluated. Likewise, the current limitations of CE in the field, such as the lack of assays involving individual proteins and targeting real-world biological samples, are brought into focus. Further strategies for method's refinement in anti-cancer metallodrug research that should ultimately take place along these lines and result in the development of high-throughput screening CE systems in the near future are finally discussed.

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Enhanced selectivity for capillary zone electrophoresis using ion-pair agents

Cited by 28 publications

References 26 publications

The effect of operating variables in microemulsion electrokinetic capillary chromatography

The effect of operating variables in microemulsion electrokinetic capillary chromatography

Analysis of Ion-Association Reaction in Aqueous Solution and Its Utilization by Capillary Zone Electrophoresis

Capillary electrophoresis in anti‐cancer metallodrug research: Advances and future challenges

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