Enhanced secondary metabolites production and antioxidant activity in postharvest Lonicera japonica Thunb. in response to UV radiation

Wei, Ning; Peng, Xin; Ma, Lai; Cui, Lei; Lü, Xiaoping; Wang, Jing; Tian, Jingkui; Li, Ximin; Wang, Wei; Zhang, Lin

doi:10.1016/j.ifset.2011.10.005

Cited by 44 publications

(29 citation statements)

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“…The contents of caffeoylquinic acids and iridoids maximumly increased 104.1 and 76.2% after 4 h UV‐B irradiation and 24 h incubation at 28°C and 80% humidity in the darkness. Moreover, anti‐oxidative assay of L. japonica showed that the anti‐oxidative activity of UV‐A or UV‐B treated groups were increased 37.2 and 51.2% with the best conditions, respectively .…”

Section: Introductionmentioning

confidence: 97%

“…However, the concentrations of the active compounds in the plant were not increased, and the quality of the plant was not fundamentally improved. Intriguingly, an innovative approach dramatically enhancing the contents of the active metabolites in the postharvest L. japonica by ultraviolet (UV) irradiation has recently been reported by our research group .…”

Section: Introductionmentioning

confidence: 99%

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Proteomics analysis of UV‐irradiated Lonicera japonicaThunb. with bioactive metabolites enhancement

Zhang

Zheng

et al. 2013

Proteomics

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A previous study showed that the contents of caffeoylquinic acids and iridoids, the major bioactive components in the postharvest Lonicera japonica Thunb., were induced by enhanced ultraviolet (UV)-A or UV-B irradiation. To clarify the UV-responsive key enzymes in the bioactive metabolites biosynthetic pathway and the related plant defense mechanism in L. japonica, 2DE in combination with MALDI-TOF/TOF MS was employed. Seventy-five out of 196 differential proteins were positively identified. Based on the functions, these proteins were grouped into nine categories, covering a wide range of molecular processes including the secondary metabolites (caffeoylquinic acids and iridoids) biosynthetic-related proteins, photosynthesis, carbohydrate and energy metabolism, stress, DNA, transport-related proteins, lipid metabolism, amino acid metabolism, cell wall. Of note is the increasing expression of 1-deoxy-d-xylulose 5-phosphate reductoisomerase and 5-enol-pyruvylshikimate-phosphate synthase, which was crucial to supply more precursor for the secondary metabolites including caffeoylquinic acids and iridoids. Thus, this study provides both the clues at the protein level for the increase of the two bioactive components upon UV irradiation and the profile of UV-responsive proteins in L. japonica.

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Section: Introductionmentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

Proteomics analysis of UV‐irradiated Lonicera japonicaThunb. with bioactive metabolites enhancement

Zhang

Zheng

et al. 2013

Proteomics

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“…A large range of metabolites with pharmaceutical potential has been investigated through a number of studies, including indole alkaloids in Catharanthus roseus [7, 14], caffeoylquinic acid and iridoids such as secologanic acid, secoxyloganin, secologanin, and (E)-aldosecologanin in Lonicera japonica Thunb. [15], and diels-alder adduct in Morus alba L . [16], which accumulated after exposures to high level of UV-B irradiation followed by the dark.…”

Section: Introductionmentioning

confidence: 99%

Enhanced metabolic process to indole alkaloids in Clematis terniflora DC. after exposure to high level of UV-B irradiation followed by the dark

et al. 2016

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BackgroundIndole alkaloids, which characteristically contain an indole nucleus, have pharmaceutical potential in a diverse range of applications. UV-B can elicit the accumulation of indole alkaloids. The indole alkaloid (6-hydroxyl-1H-indol-3-yl) carboxylic acid methyl ester with cytotoxic activity was found to accumulate in Clematis terniflora DC. leaves after exposure to high level of UV-B irradiation and the dark. However, a more in-depth analysis of the process behind this response has not yet been performed. Therefore, an integrated approach involving metabolomic, proteomic, and transcriptomic analyses is essential to detail the biosynthetic mechanisms of the regulation of indole alkaloid under binary stress.ResultsIndole alkaloid (6-hydroxyl-1H-indol-3-yl) carboxylic acid methyl ester was found to increase 7-fold in C. terniflora leaves post-treatment with high level of UV-B irradiation followed by an incubation in the dark compared with pre-treatment. Analysis by proteomics and metabolomics indicates a decrease in photosynthesis and carbohydrate metabolism, respectively. By contrast, amino acid metabolism was activated by this binary stress, and, specifically, the genes involved in the metabolic pathway converting shikimate to L-tryptophan were concurrently upregulated. Metabolites involved in indole biosynthesis (shikimate metabolic) pathway were anthranilate, indole, and L-tryptophan, which increased 2-, 441-, and 1-fold, respectively. In addition, there was an increase of 2- and 9-fold in L-serine deaminase (L-SD) and L-tryptophan synthase activity in C. terniflora leaves after exposure to high level of UV-B irradiation and the dark.Conclusions(6-hydroxyl-1H-indol-3-yl) carboxylic acid methyl ester was found to increase in response to high level of UV-B irradiation followed by an incubation in the dark, implying that indole alkaloid biosynthesis was activated in C. terniflora leaves. Analysis of perturbations in metabolism in these leaves demonstrated that amino acid metabolism was specifically activated by this binary stress. In addition, an enhancement in serine level and L-SD activity was noted, which likely leads to an accumulation of pyruvate that, in turn, supplies shikimate metabolic pathway. The genes, metabolites, and L-tryptophan synthase activity that are involved in the metabolic pathway leading from shikimate to L-tryptophan all increased under the experimental binary stress, resulting in an enhancement of indole biosynthesis (shikimate metabolic) pathway. Therefore, the metabolic process to indole alkaloids in C. terniflora was enhanced after exposure to high level of UV-B irradiation followed by the dark.Electronic supplementary materialThe online version of this article (doi:10.1186/s12870-016-0920-3) contains supplementary material, which is available to authorized users.

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“…The same quality of medicinal compounds between the two treatments indicated that increased DLI in plants exposed to SL treatments did not affect the concentration of both compounds. Generally, chlorogenic acid and luteolin are produced in plants under some types of stress condition (high light intensity, low air temperature, and UV light) (Ning et al, 2012), because these compounds function as antioxidants. Therefore, it was concluded that the environmental conditions of both treatments in this study did not impose any stress on plants, and the increased photoassimilates in leaves of plants in SL treatments may have contributed to the increased number of flower buds without affecting the concentration of medicinal compounds.…”

Section: Resultsmentioning

confidence: 99%

Effects of Supplemental Lighting on Growth and Medicinal Compounds of Japanese Honeysuckle (<I>Lonicera japonica</I> Thunb.)

Hikosaka

Iwamoto

Gotō

et al. 2017

Environmental Control in Biology

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Japanese honeysuckle is an evergreen vine that grows naturally in Japan and East Asia. The buds and leaves of this plant are used as crude drugs known as Kinginka and Nindou, respectively, in Japan and East Asia. The medicinal compounds are chlorogenic acid and luteolin, which have antiviral, anticancer, anti-inflammatory, and antioxidant activities. The optimal environmental conditions for growing Japanese honeysuckle have remained unknown thus far. The aim of this study was to investigate the effects of supplemental lighting in winter greenhouse on growth and production of medicinal compounds in Japanese honeysuckle. For that purpose, we cultivated Japanese honeysuckle plants in a greenhouse using supplemental lighting from high-pressure sodium lamps. During the experiment, the daily light integral was maintained at 10 mol m 2 d 1 by controlling the irradiation period of supplemental lighting from the evening until midnight. The total dry weight and total leaf area of plants subjected to 55 d of supplemental lighting were significantly higher than those in control plants. The number of flower buds was significantly higher in treated plants, and then, there was no difference in the concentration of chlorogenic acid and luteolin between the two groups. In conclusion, supplemental lighting is a useful method for winter cultivation of Japanese honeysuckle that increases the yield of flower buds and does not decrease the concentration of main medicinal compounds.

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Enhanced secondary metabolites production and antioxidant activity in postharvest Lonicera japonica Thunb. in response to UV radiation

Cited by 44 publications

References 25 publications

Proteomics analysis of UV‐irradiated Lonicera japonicaThunb. with bioactive metabolites enhancement

Proteomics analysis of UV‐irradiated Lonicera japonicaThunb. with bioactive metabolites enhancement

Enhanced metabolic process to indole alkaloids in Clematis terniflora DC. after exposure to high level of UV-B irradiation followed by the dark

Effects of Supplemental Lighting on Growth and Medicinal Compounds of Japanese Honeysuckle (<I>Lonicera japonica</I> Thunb.)

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