Enhanced salt tolerance of transgenic progeny of tall fescue (Festuca arundinacea) expressing a vacuolar Na+/H+ antiporter gene from Arabidopsis

Zhao, Junsheng; Zhi, Deng; Xue, Zheyong; Liu, Heng; Xia, Guangmin

doi:10.1016/j.jplph.2007.04.001

Cited by 60 publications

(25 citation statements)

References 27 publications

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“…In the present study, it was shown that the salt tolerance (Gisbert et al 2000), wheat (Xuea et al 2004), fescue (Zhao et al 2007), Arabidopsis (Li et al 2007), sugar beet (Liu et al 2008) and apple rootstock (Li et al 2010). Hamada et al (2001) successfully isolated a Na ?…”

Section: Rt-pcr Detection Of Transgenic Fig Plantsmentioning

confidence: 50%

Molecular cloning and expression of a vacuolar Na+/H+ antiporter gene (AgNHX1) in fig (Ficus carica L.) under salt stress

Metwali

Soliman

Fuller

et al. 2015

Plant Cell Tiss Organ Cult

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Soil salinity can be a major limiting factor for productivity in agriculture and forestry and in order to fully utilize saline lands productively in plantation forestry for fig production, the genetic modification of tree species for salt tolerance may be required. Na ? /H ? antiporters have been suggested to play important roles in salt tolerance in plants.Here, we isolated AgNHX1 a vacuolar Na ? /H ? antiporter from a halophytic species Atriplex gmelini and introduced it into fig (Ficus carica L.) cv. Black Mission via Agrobacterium-mediated transformation. Leaf discs explants of fig were co-cultivated for 2 days with Agrobacterium tumefaciens strain LBA 4404 harboring the binary vector pBI121 containing the AgNHX1 gene and the hpt selectable marker gene which encodes hygromycin phosphotransferase. Explants were cultured on MS medium containing 30 mg L -1 hygromycin, 3 % sucrose, 0.2 mg L -1 kinetin and 2.0 mg L -1 2,4-dichlorophenoxyacetic acid solidified with 2.5 g L -1 phytagel in darkness for callus formation. The calli were cultured on MS medium containing 2.0 mg L -1 zeatin riboside in combination with 0.4 mg L -1 indole acetic acid in the light for plant regeneration. Putative regenerated transformant shoots were confirmed by polymerase chain reaction (PCR) and Southern hybridization for the AgNHX1 gene. Reverse transcriptase polymerase chain reaction analysis indicated that the gene was highly expressed in transgenic plants, but the degree of this expression varied among transformants. Overexpression of the AgNHX1 gene conferred high tolerance to salt stress and transgenic fig plants overexpressing AgNHX1 developed normally under salinity conditions compared to those of non-transgenic plants. Salt treated transgenic plants contained high proline and K ? but less Na ? compared to non-transgenic control plants.

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Section: Rt-pcr Detection Of Transgenic Fig Plantsmentioning

confidence: 50%

Molecular cloning and expression of a vacuolar Na+/H+ antiporter gene (AgNHX1) in fig (Ficus carica L.) under salt stress

Metwali

Soliman

Fuller

et al. 2015

Plant Cell Tiss Organ Cult

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show abstract

“…These siRNAs might participate in the alternative splicing of mRNAs and represent important components of an additional level of regulation. A few reports are available in which their function has been correlated with abiotic stress, such as dehydration responsive miRNAs in rice and Arabidopsis (Sunkar and Zhu, 2004;Zhao et al, 2007;). Transcriptome analysis of drought inducible genes in Arabidopsis and rice was done employing microarray technology.…”

Section: Global Expression Profiling -Abiotic Stressmentioning

confidence: 99%

Genetic approaches towards overcoming water deficit in plants - special emphasis on LEAs

Khurana

Vishnudasan

Chhibbar

2008

Physiol Mol Biol Plants

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Water deficit arises as a result of low temperature, salinity and dehydration, thereby affecting plant growth adversely and making it imperative for plants to surmount such situations by acclimatizing/adapting at various levels. Water deficit stress results in significant changes in gene expression, mediated by interconnected signal transduction pathways that may be triggered by calcium, and regulated via ABA dependent and/or independent pathways. Hence, adaptation of plants to such stresses involves maintaining cellular homeostasis, detoxification of harmful elements and also growth alterations. Stress in general cause excess production of reactive oxygen species (ROS) and the plants overcome the same by either preventing the accumulation of ROS or by eliminating the ROS formed. Ion homeostasis includes processes such as cellular uptake, sequestration and export in conjunction with long distance transport. Requisite amounts of osmolytes are hence synthesized under stress to maintain turgor along with maintaining the macromolecular structures and also for scavenging ROS. Another noteworthy response is the accumulation of novel proteins, including enzymes involved in the biosynthesis of osmoprotectants, heat-shock proteins (HSPs), late embryogenesis abundant (LEA) proteins, antifreeze proteins, chaperones, detoxification enzymes, transcription factors, kinases and phosphatases. The LEAs belong to a redundant protein family and are highly hydrophilic, boiling-soluble, non-globular and therefore have been defined and classified accordingly. The precise function of LEAs is still unknown, but substantial evidence indicates their involvement in dessication tolerance as the expression of LEAs confers increased resistance to stress in heterologous yeast system and also significantly improves water deficit tolerance in transgenic plants. Genetic manipulation of plants towards conferring abiotic stress tolerance is a daunting task, as the abiotic stress tolerance mechanism is highly complex and various strategies have been exploited to address and evaluate the stress tolerance mechanism, and the molecular responses to water deficit via complex signaling networks. Genomic technologies have recently been useful in integrating the multigenicity of the plant stress responses through, transcriptomics, proteomics and metabolite profilling and their interactions. This review deals with the recent developments on genetic approaches for water stress tolerance in plants, with special emphasis on LEAs.

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“…Biotechnological approaches have the potential to complement or accelerate conventional breeding by extending the range of sources for valuable traits, thus offering new opportunities for tall fescue improvement. Indeed, the molecular breeding of tall fescue for tolerance to herbicides (Lee et al 2008), salt, and drought (Cao et al 2009;Zhao et al 2007) has been reported. The stress-inducible expression of two antioxidant genes (CuZnSOD and APX) simultaneously able transgenic plants to tolerate a wide range of abiotic stresses, including methyl viologen (MV), H 2 O 2 , and heavy metals (Lee et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Overexpression of a chloroplast-localized small heat shock protein OsHSP26 confers enhanced tolerance against oxidative and heat stresses in tall fescue

et al. 2011

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Small heat shock proteins are involved in stress tolerance. We previously isolated and characterized a rice cDNA clone, Oshsp26, encoding a chloroplast-localized small heat shock protein that is expressed following oxidative or heat stress. In this study, we transferred this gene to tall fescue plants by an Agrobacterium-mediated transformation system. The integration and expression of the transgene was confirmed by PCR, Southern, northern, and immunoblot analyzes. Compared to the control plants, the transgenic plants had significantly lower electrolyte leakage and accumulation of thiobarbituric acid-reactive substances when exposed to heat or methyl viologen. The photochemical efficiency of photosystem II (PSII) (Fv/Fm) in the transgenic tall fescue plants was higher than that in the control plants during heat stress (42°C). These results suggest that the OsHSP26 protein plays an important role in the protection of PSII during heat and oxidative stress in vivo.

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Enhanced salt tolerance of transgenic progeny of tall fescue (Festuca arundinacea) expressing a vacuolar Na+/H+ antiporter gene from Arabidopsis

Cited by 60 publications

References 27 publications

Molecular cloning and expression of a vacuolar Na+/H+ antiporter gene (AgNHX1) in fig (Ficus carica L.) under salt stress

Molecular cloning and expression of a vacuolar Na+/H+ antiporter gene (AgNHX1) in fig (Ficus carica L.) under salt stress

Genetic approaches towards overcoming water deficit in plants - special emphasis on LEAs

Overexpression of a chloroplast-localized small heat shock protein OsHSP26 confers enhanced tolerance against oxidative and heat stresses in tall fescue

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