Enhanced salt tolerance of transgenic poplar plants expressing a manganese superoxide dismutase from Tamarix androssowii

Wang, Yu Cheng; Qu, Guan Zheng; Li, Hong Yan; Wu, Ying; Wang, Chao; Zhao, Li; Yang, Chuan Ping

doi:10.1007/s11033-009-9884-9

Cited by 174 publications

(64 citation statements)

References 18 publications

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“…Quantitative real-time PCR analysis showed that the JcCu/Zn-SOD gene was expressed in all J. curcas tissues examined and increased under NaCl stress ( Figure 3A and B). These findings generally agree with those other studies reporting increased Cu/Zn-SOD expression in plants exposed to salt stress (Hernandez et al, 1999;Wang et al, 2010). However, no significant changes in Cu/ Zn-SOD mRNA levels were observed in other experiments under NaCl treatment (MenezesBenavente et al, 2004;Jithesh et al, 2006).…”

Section: Discussionsupporting

confidence: 92%

A Cu/Zn superoxide dismutase from Jatropha curcas enhances salt tolerance of Arabidopsis thaliana

et al. 2015

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ABSTRACT. Superoxide dismutases (SODs) are involved in protecting plants against diverse biotic and abiotic stresses. In the present study, a novel Cu/Zn-SOD gene (JcCu/Zn-SOD) was cloned from Jatropha curcas L. Quantitative reverse transcription-polymerase chain reaction analysis revealed that JcCu/Zn-SOD is constitutively expressed in different tissues of J. curcas and induced under NaCl treatment. To characterize the function of this gene with respect to salt tolerance, the construct p35S:JcCu/Zn-SOD was developed and transformed into Arabidopsis using Agrobacterium-mediated transformation. Compared with wild-type, transgenic plants over-expressing JcCu/Zn-SOD showed enhanced tolerance to salt stress during germination, seedling establishment, and growth in terms of longer root, larger rosette area, and a larger number of leaves in addition to higher SOD activity levels 2087 SOD from Jatropha curcas enhances salt tolerance ©FUNPEC-RP www.funpecrp.com.br Genetics and Molecular Research 14 (1): 2086-2098(2015 under NaCl stress. In addition, over-expression of JcCu/Zn-SOD resulted in lower monodialdehyde content in transgenic Arabidopsis compared to wild-type plants under the same NaCl stress. Therefore, JcCu/Zn-SOD can increase a plant salt stress tolerance potentially by reducing oxidant injury.

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Section: Discussionsupporting

confidence: 92%

A Cu/Zn superoxide dismutase from Jatropha curcas enhances salt tolerance of Arabidopsis thaliana

et al. 2015

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“…Since photorespiration is considered to be important to prevent ROS accumulation (Voss et al, 2013), the postulated decreased activity of the GDH/SHMT complex could be an additional cause for oxidative stress in ftsh4. Furthermore, among the proteins carbonylated preferentially in ftsh4 is the manganese superoxide dismutase, a key enzyme for eliminating mitochondrial superoxide radicals (Wang et al, 2010). This enzyme is probably inactive in ftsh4 due to oxidative modification (Qin et al, 2009), and it was found to be highly carbonylated in all the conditions examined (Table II).…”

Section: In Ftsh4 the Defective Oxphos System Is Linked To Enhancedmentioning

confidence: 94%

Lack of FTSH4 Protease Affects Protein Carbonylation, Mitochondrial Morphology, and Phospholipid Content in Mitochondria of Arabidopsis: New Insights into a Complex Interplay

et al. 2016

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FTSH4 is one of the inner membrane-embedded ATP-dependent metalloproteases in mitochondria of Arabidopsis (Arabidopsis thaliana). In mutants impaired to express FTSH4, carbonylated proteins accumulated and leaf morphology was altered when grown under a short-day photoperiod, at 22°C, and a long-day photoperiod, at 30°C. To provide better insight into the function of FTSH4, we compared the mitochondrial proteomes and oxyproteomes of two ftsh4 mutants and wild-type plants grown under conditions inducing the phenotypic alterations. Numerous proteins from various submitochondrial compartments were observed to be carbonylated in the ftsh4 mutants, indicating a widespread oxidative stress. One of the reasons for the accumulation of carbonylated proteins in ftsh4 was the limited ATP-dependent proteolytic capacity of ftsh4 mitochondria, arising from insufficient ATP amount, probably as a result of an impaired oxidative phosphorylation (OXPHOS), especially complex V. In ftsh4, we further observed giant, spherical mitochondria coexisting among normal ones. Both effects, the increased number of abnormal mitochondria and the decreased stability/activity of the OXPHOS complexes, were probably caused by the lower amount of the mitochondrial membrane phospholipid cardiolipin. We postulate that the reduced cardiolipin content in ftsh4 mitochondria leads to perturbations within the OXPHOS complexes, generating more reactive oxygen species and less ATP, and to the deregulation of mitochondrial dynamics, causing in consequence the accumulation of oxidative damage.

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“…The plant antioxidant system consists of ROS-scavenging enzymes, such as superoxide dismutase (SOD), catalase (CAT), and ascorbate peroxidase (APX), as well as low-molecular-weight antioxidants like glutathione, ascorbate, carotenoids, metallothionein, etc (Table 1). Analysis with transgenic plants overexpressing these antioxidant genes revealed that maintenance of a high antioxidant capacity in cells is linked to increased tolerance against various adverse conditions (Guo et al, 2009;Jayaraj & Punja, 2008;Tseng et al, 2007;Wang et al, 2010). Heavy metal stresses can shift the cellular balance of free radical homeostasis into terrible accumulation of H 2 O 2 .…”

Section: Heavy Metal-induced Oxidative Stress and Stress Tolerance: Cmentioning

confidence: 99%

Towards Understanding Plant Response to Heavy Metal Stress

Zhao¹,

Chu²

2011

Abiotic Stress in Plants - Mechanisms and Adaptations

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IntroductionMetals like zinc, iron and copper are essential micronutrients required for a wide range of physiological processes in all plant organs for the activities of various metal-dependent enzymes and proteins. However, they can also be toxic at elevated levels. Metals like arsenic, mercury, cadmium and lead are nonessential and potentially highly toxic. Once the cytosolic metal concentration in plant turns out of control, phytotoxicity of heavy metal inhibits transpiration and photosynthesis, disturbs carbohydrate metabolism, and drives the secondary stresses like nutrition stress and oxidative stress, which collectively affect the plant development and growth (Krämer & Clemens, 2005). Plants have developed a complex network of highly effective homeostatic mechanisms that serve to control the uptake, accumulation, trafficking, and detoxification of metals. Components of this network have been identified continuously, including metal transporters in charge of metal uptake and vacuolar transport; chelators involved in metal detoxification via buffering the cytosolic metal concentrations; and chaperones helping delivery and trafficking of metal ions (Clemens, 2001). This chapter summarizes heavy metal stress and detoxification in plant. Special focus is given to metallothionein, yet vacuolar metal transporters, phytochelatins as well as certain organic acids, amino acids, and chaperones are also addressed with recent advances. Besides, heavy metal-induced oxidative stress and tolerance as an example of abiotic stress cross-talk will be discussed.

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Enhanced salt tolerance of transgenic poplar plants expressing a manganese superoxide dismutase from Tamarix androssowii

Cited by 174 publications

References 18 publications

A Cu/Zn superoxide dismutase from Jatropha curcas enhances salt tolerance of Arabidopsis thaliana

A Cu/Zn superoxide dismutase from Jatropha curcas enhances salt tolerance of Arabidopsis thaliana

Lack of FTSH4 Protease Affects Protein Carbonylation, Mitochondrial Morphology, and Phospholipid Content in Mitochondria of Arabidopsis: New Insights into a Complex Interplay

Towards Understanding Plant Response to Heavy Metal Stress

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