Enhanced saccharification of sugarcane bagasse by the optimization of low concentration of NaOH and ammonia pretreatment

Wang, Jue; Xu, Yuan; Tang, Baoying; Zeng, Minxia; Liang, Zhiquan; Jiang, Cuifeng; Lin, Jianghai; Xiao, Wenjuan; Liu, Zehuan

doi:10.1016/j.indcrop.2021.114016

Cited by 14 publications

(7 citation statements)

References 18 publications

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“…It can be seen that ultrasonic-assisted NaOH and ammonia water mixed alkali pretreatment resulted in higher xylose content. Wang et al ( 31 ) obtained a total reducing sugar concentration of 16.23 g/L through NaOH and ammonia water pretreatment of enzymatic hydrolysis from sugarcane bagasse.…”

Section: Resultsmentioning

confidence: 99%

Ultrasonic-Assisted Dual-Alkali Pretreatment and Enzymatic Hydrolysis of Sugarcane Bagasse Followed by Candida tropicalis Fermentation to Produce Xylitol

et al. 2022

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In this work, the investigation mainly focused on ultrasonic-assisted dual-alkali pretreatment and enzymatic hydrolysis of sugarcane bagasse followed by Candida tropicalis fermentation to produce xylitol. The results showed that the combination of NaOH and ammonia water had the best effect by comparing the effects of the four single-alkali (NaOH, KOH, ammonia water, Ca(OH)2) and their mixed double-alkali pretreatments on xylose content. Then, the optimal conditions for ultrasonic-assisted pretreatment and enzymatic hydrolysis of sugarcane bagasse were obtained by response surface methodology. When the ratio of NaOH and ammonia water was 2:1, the mixed alkali concentration (v/v) was 17%, the ultrasonic temperature was 45°C, the ultrasonic power was 300 W, and the ultrasonic time was 40 min, the content of xylose reached a maximum of 2.431 g/L. Scanning electron microscopy showed that sugarcane bagasse by ultrasonic-assisted alkali pretreatment aggravated with more folds and furrows. Moreover, the fermentation results showed that the concentration ratio of enzymatic hydrolysate of sugarcane bagasse affected the xylitol yield, and when concentrated three times, the highest yield of xylitol (54.42%) was obtained.

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Section: Resultsmentioning

confidence: 99%

Ultrasonic-Assisted Dual-Alkali Pretreatment and Enzymatic Hydrolysis of Sugarcane Bagasse Followed by Candida tropicalis Fermentation to Produce Xylitol

et al. 2022

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“…It was reported by many studies that alkali-based pretreatment could remove lignin in lignocellulose [ 26 , 27 , 41 , 42 ]. Li et al [ 38 ] reported that the addition of Na 2 SO 3 in alkali-based pretreatment improved the recovery of carbohydrates and the removal of lignin.…”

Section: Resultsmentioning

confidence: 99%

“…Research has reported that lignin removal was an effective method for improving enzymatic hydrolysis performance. Some methods have been investigated for removing lignin in lignocellulose; for example, NaOH pretreatment [ 26 , 27 ], organic solvent pretreatment with formic acid or acetic acid [ 28 ], and sulfite pretreatment [ 29 , 30 , 31 , 32 ]. The sulfite pretreatment was regarded as a potential process for improving the enzymatic hydrolysis efficiency of lignocellulose and has been used to pretreat corncob [ 29 ], softwood [ 33 ], oil palm empty fruit bunch [ 32 ], and so on.…”

Section: Introductionmentioning

confidence: 99%

Pretreatment Strategies to Enhance Enzymatic Hydrolysis and Cellulosic Ethanol Production for Biorefinery of Corn Stover

Sun

Zhao

et al. 2022

IJMS

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There is a rising interest in bioethanol production from lignocellulose such as corn stover to decrease the need for fossil fuels, but most research mainly focuses on how to improve ethanol yield and pays less attention to the biorefinery of corn stover. To realize the utilization of different components of corn stover in this study, different pretreatment strategies were used to fractionate corn stover while enhancing enzymatic digestibility and cellulosic ethanol production. It was found that the pretreatment process combining dilute acid (DA) and alkaline sodium sulfite (ASS) could effectively fractionate the three main components of corn stover, i.e., cellulose, hemicellulose, and lignin, that xylose recovery reached 93.0%, and that removal rate of lignin was 85.0%. After the joint pretreatment of DA and ASS, the conversion of cellulose at 72 h of enzymatic hydrolysis reached 85.4%, and ethanol concentration reached 48.5 g/L through fed-batch semi-simultaneous saccharification and fermentation (S-SSF) process when the final concentration of substrate was 18% (w/v). Pretreatment with ammonium sulfite resulted in 83.8% of lignin removal, and the conversion of cellulose and ethanol concentration reached 86.6% and 50 g/L after enzymatic hydrolysis of 72 h and fed-batch S-SSF, respectively. The results provided a reference for effectively separating hemicellulose and lignin from corn stover and producing cellulosic ethanol for the biorefinery of corn stover.

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“…Pretreatment can decompose or remove lignin and degrade hemicellulose by changing the microstructure, macrostructure, and chemical composition of materials, changing cellulose crystallinity, increasing the contact area of enzymes, and improving the efficiency of cellulose hydrolysis (Niju and Swathika, 2019 ; Tinôco et al, 2021 ; Wang et al, 2021 ). Traditional pretreatment methods for lignocellulosic biomass included physical (mechanical), chemical (acid and alkali), and biological (mainly enzymes, fungal, and microbial) pretreatments (Cai et al, 2021 ; Sun et al, 2021 ).…”

Section: Introductionmentioning

confidence: 99%

“…Recently, alkali pretreatment achieved by NaOH is the most widely used and effective method for various lignocelluloses to enhance their cellulosic utilization through addressing textural recalcitrance (Wang et al, 2021 ). NaOH pretreatment can destroy the ester and ether bonds of lignin and increase the porosity of the biomass (Rezania et al, 2020 ; Zhang H. et al, 2020 ; Zhang J. et al, 2020 ; Wang et al, 2021 ). In addition, NaOH can also dissociate into Na + and OH − to further promote the hydrolysis rate (Tang et al, 2023 ).…”

Section: Introductionmentioning

confidence: 99%

Enhancing biomass conservation and enzymatic hydrolysis of sweet sorghum bagasse by combining pretreatment with ensiling and NaOH

Zhao,

Li,

Sumpradit

et al. 2024

Front. Microbiol.

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Lignocellulosic pretreatment is an important stage in biomass utilization, which usually requires high input. In this study, a low-cost method using combined ensiling and NaOH was developed for lignocellulosic pretreatment. Sweet sorghum bagasse (SSB) was ensiled for 21 days and then treated with diluted NaOH (0%, 1%, and 2%) for fermentation. The results showed that the application of Lactobacillus plantarum (L) reduced fermentation losses of the silages, mainly low water-soluble carbohydrate (WSC) and ammonia nitrogen loss. Meanwhile, the application of Lactobacillus plantarum and ensiling enzyme (LE) promoted lignocellulosic degradation, as evidenced by low neutral detergent fiber (NDF), acid detergent fiber (ADF), lignin (ADL), and hemicellulosic (HC) contents. The dominant bacterial genera were Lactobacillus, uncultured_bacterium_f_Enterobacteriaceae, and Pantoea after silage, which corresponded to the higher lactic acid and acetic contents and lower pH. The reducing sugar yields of SSB increased after combined pretreatment of silage and NaOH and were further enhanced by the 2% NaOH application, as evidenced by the high reducing sugar yield and microstructure damage, especially in the L-2% NaOH group and the LE-2% NaOH group, in which the reducing sugar yields were 87.99 and 94.45%, respectively, compared with those of the no additive control (CK)-0 NaOH group. Therefore, this study provides an effective method for SSB pretreatment to enhance biomass conservation.

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Enhanced saccharification of sugarcane bagasse by the optimization of low concentration of NaOH and ammonia pretreatment

Cited by 14 publications

References 18 publications

Ultrasonic-Assisted Dual-Alkali Pretreatment and Enzymatic Hydrolysis of Sugarcane Bagasse Followed by Candida tropicalis Fermentation to Produce Xylitol

Ultrasonic-Assisted Dual-Alkali Pretreatment and Enzymatic Hydrolysis of Sugarcane Bagasse Followed by Candida tropicalis Fermentation to Produce Xylitol

Pretreatment Strategies to Enhance Enzymatic Hydrolysis and Cellulosic Ethanol Production for Biorefinery of Corn Stover

Enhancing biomass conservation and enzymatic hydrolysis of sweet sorghum bagasse by combining pretreatment with ensiling and NaOH

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