Enhanced resistance to sheath blight by constitutive expression of infection-related rice chitinase in transgenic elite indica rice cultivars

Datta, Karabi; Tu, J. C.; Oliva, Norman; Oña, I.; Velazhahan, R.; Mew, T. W.; Muthukrishnan, Subbaratnam; Datta, Swapan K.

doi:10.1016/s0168-9452(00)00413-1

Cited by 195 publications

(105 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…A few examples of transgenes for pest protection that have already been used in rice transformation and could become available in Vietnam and elsewhere include Bt and cowpea trypsin inhibitor genes for resistance to lepidopteran larvae (High et al, 2004;Huang et al, 2005), lectin genes for resistance to sucking insects (Nagadhara et al, 2003;Saha et al, 2006), chitinase or allene oxide synthase genes for resistance to fungal diseases (Datta et al, 2001;Mei et al, 2006), and trangenes for resistance to bacterial blight (Zai et al, 2004). Other transgenic traits that could be released separately or in combination with various types of pest resistance include resistance to commonly used herbicides, greater tolerance of abiotic stresses, and enhanced production of pro-vitamin A (e.g., Lu and Snow, 2005).…”

Section: 15mentioning

confidence: 99%

Shared flowering phenology, insect pests, and pathogens among wild, weedy, and cultivated rice in the Mekong Delta, Vietnam: implications for transgenic rice

Cohen

Arpaia

Lan

et al. 2008

Environ. Biosafety Res.

View full text Add to dashboard Cite

Many varieties of transgenic rice are under development in countries where wild and weedy relatives co-occur with the crop. To evaluate possible risks associated with pollen-mediated transgene dispersal, we conducted a two-year survey in Vietnam to examine overlapping flowering periods of rice (Oryza sativa L.), weedy rice (O. sativa), and wild rice (O. rufipogon Griff.), all of which are inter-fertile. We surveyed populations in two regions of the Mekong Delta, northern and southern, and at three sites in each of three habitats per region: fresh water, saline water, and acid sulfate soil. Weedy rice frequently flowered simultaneously with neighboring cultivated rice plants. Flowering was more seasonal in wild rice and often peaked in November and December. Peak flowering times of wild rice overlapped with adjacent rice fields at all of the saline sites and half of the acid sulfate sites. The longer flowering season of wild rice ensured that crop-to-wild gene flow was possible in fresh water habitats as well. Our second objective was to determine whether wild and weedy rice populations are exposed to pests that could be targeted by future transgenes, which may then provide fitness benefits. These populations shared many pathogen and insect herbivore species with cultivated rice (leaffolder, locust, cricket, planthoppers, rice bug, stem borer, sheath blight, blast, bacterial leaf blight, and brown spot). Damage by leaffolders and locusts was the most frequently observed insect feeding damage on all three rice types. Indicator species analysis revealed that most of the insect herbivores were associated with particular habitats, demonstrating the importance of broad geographic sampling for transgenic rice risk assessment. These survey data and the strong likelihood of gene flow from cultivated rice suggest that further studies are needed to examine the effects of transgenic traits such as resistance to pests on the abundance of wild and weedy rice.

show abstract

Section: 15mentioning

confidence: 99%

Shared flowering phenology, insect pests, and pathogens among wild, weedy, and cultivated rice in the Mekong Delta, Vietnam: implications for transgenic rice

Cohen

Arpaia

Lan

et al. 2008

Environ. Biosafety Res.

View full text Add to dashboard Cite

show abstract

“…Chitinase class-1 (PR-3) increased resistance in rice to R. solani and Magnaporthe grisea (Herbert) M.E. Barr (Datta et al 2001). Many functions have been attributed to PR-5 genes including protection against osmotic stress (Kononowicz et al 1992), and freezing tolerance (Hon et al 1995).…”

Section: Introductionmentioning

confidence: 99%

Expression of the pathogenesis related proteins, NH-1, PAL, and lipoxygenase in the iranian Tarom and Khazar rice cultivars, in reaction to Rhizoctonia solani – the causal agent of rice sheath blight

Sayari¹,

Babaeizad²,

Ghanbari³

et al. 2014

Journal of Plant Protection Research

View full text Add to dashboard Cite

Pathogenesis related (PR) genes of rice are among the most important defense genes in the interaction of rice with pathogens. In this study, the role of NH-1, several PR genes, phenylalanine ammonia-lyase (PAL), and lipoxygenase in the defense responses of rice against Rhizoctonia solani, the causal agent of rice sheath blight disease, was evaluated. The Tarom and Khazar cultivars (cvs), as resistant and susceptible genotypes, respectively, were used. The expression rate of defense genes in two-week-old seedlings inoculated with a virulent isolate of R. solani AG-I-1 A was investigated. The lesions in the Tarom cv were less than half the size of those on the Khazar cv. The expression scripts of the genes were calculated by quantative Real-Time PCR (RT-PCR). Results showed that the expression rate of all genes in the resistant cultivar was higher than that of the susceptible genotype, post inoculation. Analysis of data by the t-Student test also indicated significant differences in the expression level of the genes between Khazar and Tarom.The results of this study suggest that the investigated genes are involved in the resistance responses of rice against the sheath blight agent. For the first time, the induction of PR-5, PR-9, PR-10, PR-12, PR-13, and NH-1 was observed in this study in the resistant and susceptible Iranian cultivars of rice following attacks by R. solani.

show abstract

“…The most common cytosolic type I RIP from the endosperm of cereal grains do not act on plant ribosomes but can affect foreign ribosomes, such as those of fungi (Stirpe et al, 1992;Hartley et al, 1996). Several transgenic plants harbouring PR genes have already been produced, aiming at producing fungal disease-resistant varieties in a variety of crops (Jach et al, 1995;Tabei et al, 1997;Bieri et al, 2000;Datta et al, 2001;Jayaraj & Punja, 2007;Xiao et al, 2007;Almasia et al, 2008;Esfahani et al, 2010), including the transgenic tobacco that was engineered with the RIP encoding DNA sequence of barley and exhibits resistance to R. solani (Logeman et al, 1992). Many of the PR proteins may act synergistically in vivo and also enhanced inhibition of fungal growth when tested in combination in vitro (Jach et al, 1995;Chye et al, 2005).…”

Section: Bacterial Strain and Growth Conditionsmentioning

confidence: 99%

Enhanced resistance to Rhizoctonia solani by combined expression of chitinase and Ribosome Inactivating Protein in transgenic potatoes (Solanum tuberosum L.)

Mahmoud¹,

Chikh-Rouhou²,

Boughalleb³

et al. 2012

Span. j. agric. res.

View full text Add to dashboard Cite

Potato (Solanum tuberosum L.) is susceptible to many fungal pathogens including Rhizoctonia solani. In the present study, the potato cultivar Desirée was transformed via Agrobacterium tumefaciens strain GV3101 containing the binary plasmid pGJ132 harboring both the chitinase (chiA) and rip30 genes. The potato leaf disc was used as an explant for transformation. PCR, Southern blot and Western blot were used for characterization of the transgenic plants. In this study it was shown that not all the plants developed in selective medium were positive for the corresponding gene using the PCR technique. Southern blot analysis confirmed that transgenic plants integrated 2-3 copies of chiA and rip30 genes respectively into their genome. The expression of the CHIA and RIP30 proteins was confirmed in the leaf extracts of the transgenic clones by Western blot analysis. Transgenic potato plants expressing rip30 and chiA genes showed enhanced resistance to R. solani in a greenhouse assay.Additional key words: fungal resistance; genes; proteins; southern blot; western blot. ResumenResistencia aumentada a Rhizoctonia solani por la expresión combinada de quitinasa y proteínas inactivantes de los ribosomas en patatas transgénicas La patata (Solanum tuberosum L.) es susceptible a muchos hongos fitopatógenos, incluyendo Rhizoctonia solani. En el presente estudio, se transformó el cultivar de patata 'Desirée' mediante Agrobacterium tumefaciens, cepa GV3101, que contiene el plásmido binario pGJ132 que alberga los genes quitinasa (chiA) y rip30. Se utilizaron discos de hojas como explante para la transformación de plantas. Se utilizaron las técnicas de PCR, Southern y Western blot para la caracterización de las plantas transgénicas. En este estudio se demostró, mediante PCR, que no todas las plantas que se desarrollaron en medio selectivo fueron positivas para el gen correspondiente. El análisis de Southern blot confirmó que las plantas transgénicas integraron en su genoma 2-3 copias de los genes chiA y rip30. Se llevó a cabo un ensayo de invernadero para evaluar la resistencia a R. solani de los clones transgénicos que expresan los transgenes. Las plantas transgénicas que expresan los genes rip30 y chiA mostraron una resistencia completa a R. solani.

show abstract

Enhanced resistance to sheath blight by constitutive expression of infection-related rice chitinase in transgenic elite indica rice cultivars

Cited by 195 publications

References 28 publications

Shared flowering phenology, insect pests, and pathogens among wild, weedy, and cultivated rice in the Mekong Delta, Vietnam: implications for transgenic rice

Shared flowering phenology, insect pests, and pathogens among wild, weedy, and cultivated rice in the Mekong Delta, Vietnam: implications for transgenic rice

Expression of the pathogenesis related proteins, NH-1, PAL, and lipoxygenase in the iranian Tarom and Khazar rice cultivars, in reaction to Rhizoctonia solani – the causal agent of rice sheath blight

Enhanced resistance to Rhizoctonia solani by combined expression of chitinase and Ribosome Inactivating Protein in transgenic potatoes (Solanum tuberosum L.)

Contact Info

Product

Resources

About