Enhanced recovery of Arcobacter spp. using NaCl in culture media and re-assessment of the traits of Arcobacter marinus and Arcobacter halophilus isolated from marine water and shellfish

Salas-Massó, Nuria; Andrée, Karl B.; Furones, M. Dolores; Figueras, María José

doi:10.1016/j.scitotenv.2016.05.197

Cited by 27 publications

(50 citation statements)

References 43 publications

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“…The fast growing characteristics of A. butzleri in enrichment culture may mask the abundance of other species (as well as when enriching for campylobacters (Banting et al, 2016) such as A. cryaerophilus (Houf et al, 2002;Collado and Figueras, 2011;Levican, 2016;Levican et al, 2016). A. cryaerophilus was not recovered using medium supplemented with NaCl in the study of Salas-Massó et al (2016), despite being the second most abundant species using the Arcobacter-CAT medium without salt, indicating that A. cryaerophilus cannot tolerate 2.5% NaCl. Therefore, the range of species recovered from the environment may depend upon the culture media and incubation conditions employed.…”

Section: Physical Description Of Arcobacter Speciesmentioning

confidence: 96%

“…In fact, the use of Arcobacter-CAT broth supplemented with 2.5% NaCl (w/v) with a subsequent culture on marine agar after passive filtration enabled the recovery of 52 new Arcobacter marinus and 6 Arcobacter halophilus isolates. These two species had previously only been known from single strains recovered the former from a mix of seaweeds, starfish and seawater from Korea and the latter from a hypersaline lagoon in Hawaii (Salas-Massó et al, 2016). The same approaches enabled recovery of several new species from shellfish and water, which have not yet been described (Salas-Massó et al, 2016).…”

Section: New Variantsmentioning

confidence: 99%

“…An additional atypical member of the genus, A. halophilus, is an obligate halophile that requires at least 2% NaCl in media to grow (Donachie et al, 2005). Recently, A. halophilus and A. marinus were recovered from water and shellfish using Arcobacter-CAT enrichment liquid medium supplemented with 2.5% NaCl (w/v) followed by culturing on Marine Agar (Salas-Massó et al, 2016). The introduction of this protocol has allowed the identification of 40% more Arcobacterpositive samples from seawater than the conventional method without the salt supplement (Salas-Massó et al, 2016).…”

Section: Physical Description Of Arcobacter Speciesmentioning

confidence: 99%

“…These two species had previously only been known from single strains recovered the former from a mix of seaweeds, starfish and seawater from Korea and the latter from a hypersaline lagoon in Hawaii (Salas-Massó et al, 2016). The same approaches enabled recovery of several new species from shellfish and water, which have not yet been described (Salas-Massó et al, 2016). Additionally, a separate new species (A. porcinus) has been identified amongst strains included in the description of A. thereius (Figueras et al, 2017).…”

Section: New Variantsmentioning

confidence: 99%

“…A. molluscorum and A. bivalviorum have been recovered from mussels, clams, cockles and oysters (Figueras et al, 2012;Salas-Massó et al, 2016); A. venerupis from clams (Levican et al, 2012) and A. ebronensis, A. ellisii, A. bivalviorum and A. mytili have all been recovered from mussels (Collado et al, 2009a;Figueras et al, 2011a;Figueras et al, 2011b;. Recently, A. marinus, A. halophilus and A. mytili have been recovered from oysters (Salas-Massó et al, 2016).…”

Section: Shellfishmentioning

confidence: 99%

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Arcobacter

Banting¹,

Salvat²

2019

Water and Sanitation for the 21st Century: Health and Microbiological Aspects of Excreta and Wastewater Management (Global Wate

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Section: Physical Description Of Arcobacter Speciesmentioning

confidence: 96%

Section: New Variantsmentioning

confidence: 99%

Section: Physical Description Of Arcobacter Speciesmentioning

confidence: 99%

Section: New Variantsmentioning

confidence: 99%

Section: Shellfishmentioning

confidence: 99%

See 3 more Smart Citations

Arcobacter

Banting¹,

Salvat²

2019

Water and Sanitation for the 21st Century: Health and Microbiological Aspects of Excreta and Wastewater Management (Global Wate

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Diversity, enumeration, and isolation of Arcobacter spp. in the giant abalone, Haliotis gigantea

et al. 2019

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Arcobacter have been frequently detected in and isolated from bivalves, but there is very little information on the genus Arcobacter in the abalone, an important fishery resource. This study aimed to investigate the genetic diversity and abundance of bacteria from the genus Arcobacter in the Japanese giant abalone, Haliotis gigantea, using molecular methods such as Arcobacter‐specific clone libraries and fluorescence in situ hybridization (FISH). Furthermore, we attempted to isolate the Arcobacter species detected. Twelve genotypes of clones were obtained from Arcobacter‐specific clone libraries. These sequences are not classified with any other known Arcobacter species including pathogenic Arcobacter spp., A. butzleri, A. skirrowii, and A. cryaerophilus, commonly isolated or detected from bivalves. From the FISH analysis, we observed that ARC94F‐positive cells, presumed to be Arcobacter, accounted for 6.96 ± 0.72% of all EUB338‐positive cells. In the culture method, three genotypes of Arcobacter were isolated from abalones. One genotype had a similarity of 99.2%–100.0% to the 16S rRNA gene of Arcobacter marinus, while the others showed only 93.3%–94.3% similarity to other Arcobacter species. These data indicate that abalones carry Arcobacter as a common bacterial genus which includes uncultured species.

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Simultaneous bioelectricity generation, desalination, organics degradation, and nitrogen removal in air–cathode microbial desalination cells

Zhao²,

et al. 2020

SN Appl. Sci.

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In this study, a three-chamber air-cathode microbial desalination cell (AMDC) was constructed using activated sludge as the inoculating microbial source and carbon cloth as the electrode. The simultaneous bioelectricity generation, desalination, degradation of organic compounds, and nitrogen removal in the AMDC having different electrode spacings (16, 12, and 8 cm) provided an insight into the microbial community. The experimental results showed that with a gradual decrease in the electrode spacing, electricity production and desalination performance first increased and then decreased. While AMDC1 with 16 cm electrode spacing became active faster than other cells and reached a peak voltage of 598 mV on the tenth day, AMDC2 with 12 cm electrode spacing showed the best start-up performance with a maximum output voltage of 645 mV, maximum power density of 214.7 mW/m 3 , and minimum resistance of 516.9 Ω. AMDC3 with 8 cm electrode spacing reached a peak voltage of 598 mV, which was between those of AMDC1 and AMDC2. The three AMDCs were run until the end of the cycle, and there was no significant difference in their ammonium removal rate and chemical oxygen demand. For AMDC1-3, the desalination rates were 84.86%, 87.71%, and 83.43%, and the Coulomb efficiencies were 17.29%, 18.60%, and 17.95%, respectively. Further, scanning electron microscopy showed that a large number of microorganisms could attach to the surface of the anode carbon cloth electrode; 16S rRNA sequencing showed that the typical electrogenic microbial communities were Bacillus (11.6%) and Arcobacter (9.6%). As the poor performance of the AMDC was primarily due to a lack of electrogenic microorganisms in the active function of the anode chamber, screening the functional microorganisms provided a reference for optimizing and amplifying the application of microbial desalination cells in practical research.

show abstract

Enhanced recovery of Arcobacter spp. using NaCl in culture media and re-assessment of the traits of Arcobacter marinus and Arcobacter halophilus isolated from marine water and shellfish

Cited by 27 publications

References 43 publications

Arcobacter

Arcobacter

Diversity, enumeration, and isolation of Arcobacter spp. in the giant abalone, Haliotis gigantea

Simultaneous bioelectricity generation, desalination, organics degradation, and nitrogen removal in air–cathode microbial desalination cells

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