Enhanced protection against Q fever in BALB/c mice elicited by immunization of chloroform-methanol residue of Coxiella burnetii via intratracheal inoculation

Feng, Jian; Hu, Xueyuan; Fu, Mengjiao; Dai, Lupeng; Yu, Yonghui; Luo, Wenbo; Zhao, Zhenze; Lu, Zhiyu; Du, Zongmin; Zhou, Dongsheng; Wen, Bin; Jiao, Jun; Xiong, Xiaolu

doi:10.1016/j.vaccine.2019.08.041

Cited by 26 publications

(25 citation statements)

References 35 publications

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“…Immunization via aerosolized i.t. can induce higher levels of mucosal antibodies, which play an important role in improving bacterial clearance and protective efficacy (Feng et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

“…Immunization via aerosolized i.t. can induce higher levels of mucosal antibodies, which play an important role in improving bacterial clearance and protective efficacy (Feng et al, 2019 ). Vaccination through this route overcomes the disadvantages of intranasal and intratracheal immunization, such as drug loss in the nose, throat, and upper airways, and invasive injury, making it a preferable immunization route.…”

Section: Discussionmentioning

confidence: 99%

“…For i.t. infection, 50 μl of EV76-B-SHU or EV76-B-SHUΔ pla was aerosolized and delivered into the lungs of mice by a Micro Sprayer (Huironghe Company, Beijing, China) with the help of laryngoscope (Huironghe Company, Beijing, China) (Feng et al, 2019 ). For s.c. infection 100 μl of EV76-B-SHU or EV76-B-SHUΔ pla was subcutaneously injected into the inner thigh of mouse.…”

Section: Methodsmentioning

confidence: 99%

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Construction of a Live-Attenuated Vaccine Strain of Yersinia pestis EV76-B-SHUΔpla and Evaluation of Its Protection Efficacy in a Mouse Model by Aerosolized Intratracheal Inoculation

Feng

Deng

et al. 2020

Front. Cell. Infect. Microbiol.

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Plague, which is caused by Yersinia pestis, is one of the most dangerous infectious diseases. No FDA-approved vaccine against plague is available for human use at present. To improve the immune safety of Y. pestis EV76 based live attenuated vaccine and to explore the feasibility of aerosolized intratracheal inoculation (i.t.) route for vaccine delivery, a plasminogen activator protease (pla) gene deletion mutant of the attenuated Y. pestis strain EV76-B-SHU was constructed, and its residual virulence and protective efficacy were evaluated in a mouse model via aerosolized intratracheal inoculation (i.t.) or via subcutaneous injection (s.c.). The residual virulence of EV76-B-SHU pla was significantly reduced compared to that of the parental strain EV76-B-SHU following i.t. and s.c. infection. The EV76-B-SHU pla induced higher levels of mucosal antibody sIgA in the bronchoalveolar lavage fluid of mice immunized by i.t. but not by s.c.. Moreover, after lethal challenge with Y. pestis biovar Microtus strain 201 (avirulent in humans), the protective efficacy and bacterial clearance ability of the EV76-B-SHU pla-i.t. group were comparable to those of the EV76-B-SHU pla-s.c. and EV76-B-SHU immunized groups. Thus, the EV76-B-SHU pla represents an excellent live-attenuated vaccine candidate against pneumonic plague and aerosolized i.t. represents a promising immunization route in mouse model.

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“…Immunization via aerosolized i.t. can induce higher levels of mucosal antibodies, which play an important role in improving bacterial clearance and protective efficacy (Feng et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

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Construction of a Live-Attenuated Vaccine Strain of Yersinia pestis EV76-B-SHUΔpla and Evaluation of Its Protection Efficacy in a Mouse Model by Aerosolized Intratracheal Inoculation

Feng

Deng

et al. 2020

Front. Cell. Infect. Microbiol.

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“…C . burnetii PI strains that isolated in China, including Xinqiao, Qiyi, Yaan, and H-11 strain [ 18 , 19 ], as well as C . burnetii PII strains, including NMII and Grita strain, were initially propagated in YS for 7–9 passages.…”

Section: Methodsmentioning

confidence: 99%

“…All bacteria were preserved in our laboratory. C. burnetii PI strains that isolated in China, including Xinqiao, Qiyi, Yaan, and H-11 strain [18,19], as well as C. burnetii PII strains, including NMII and Grita strain, were initially propagated in YS for 7-9 passages. Then the strains were transferred to ACCM-2 for another five passages in order to adapt to the cell-free culture system and to synchronise the passage numbers of the strains [20,21].…”

Section: Ethicsmentioning

confidence: 99%

Development and evaluation of an up-converting phosphor technology-based lateral flow assay for rapid and quantitative detection of Coxiella burnetii phase I strains

et al. 2020

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Background: Coxiella burnetii is an obligate intracellular Gram-negative bacterium that causes a zoonotic disease commonly called Q fever globally. In this study, an up-converting phosphor technology-based lateral flow (UPT-LF) assay was established for the rapid and specific detection of phase I strains of C. burnetii. Results: Specific monoclonal antibodies (10B5 and 10G7) against C. burnetii phase I strains were prepared and selected for use in the UPT-LF assay by the double-antibody-sandwich method. The detection sensitivity of the Coxiella-UPT-LF was 5 × 10 4 GE/ml for a purified C. burnetii phase I strain and 10 ng/ml for LPS of C. burnetii Nine Mile phase I (NMI). Good linearity was observed for C. burnetii phase I and NMI LPS quantification (R 2 ≥ 0.989). The UPT-LF assay also exhibited a high specificity to C. burnetii, without false-positive results even at 10 8 GE/ml of non-specific bacteria, and good inclusivity for detecting different phase I strains of C. burnetii. Moreover, the performance of the Coxiella-UPT-LF assay was further confirmed using experimentally and naturally infected samples. Conclusions: Our results indicate that Coxiella-UPT-LF is a sensitive and reliable method for rapid screening of C. burnetii, suitable for on-site detection in the field.

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Synthetic Particulate Subunit Vaccines for the Prevention of Q Fever

Sam,

Plain,

Chen

et al. 2024

Adv Healthcare Materials

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Coxiella burnetti is an intracellular bacterium that causes Q fever, a disease of worldwide importance. Q‐VAX®, the approved human Q fever vaccine, is a whole cell vaccine associated with safety concerns. Here we developed a safe particulate subunit vaccine candidate that is ambient‐temperature stable and can be cost‐effectively manufactured. We bioengineered endotoxin‐free Escherichia coli to efficiently self‐assemble biopolymer particles (BPs) that are densely coated with either strings of 18 T‐cell epitopes (COX‐BP) or two full‐length immunodominant antigens (YbgF‐BP‐Com1) all derived from C. burnetii. BP vaccine candidates were ambient‐temperature stable. Safety and immunogenicity were confirmed in mice and guinea pig models. YbgF‐BP‐Com1 elicited specific and strong humoral immune responses in guinea pigs with IgG titers that were at least 1000 times higher than those induced by Q‐VAX®. BP vaccine candidates were not reactogenic. After challenge with C. burnetii, YbgF‐BP‐Com1 vaccine led to reduced fever responses and pathogen burden in the liver and the induction of proinflammatory cytokines IL‐12 and IFN‐γ inducible protein (IP‐10) when compared to negative control groups. These data suggested that YbgF‐BP‐Com1 induces functional immune responses reducing infection by C. burnetii. Collectively, our findings illustrate the potential of BPs as an effective antigen carrier for Q fever vaccine development.This article is protected by copyright. All rights reserved

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Enhanced protection against Q fever in BALB/c mice elicited by immunization of chloroform-methanol residue of Coxiella burnetii via intratracheal inoculation

Cited by 26 publications

References 35 publications

Construction of a Live-Attenuated Vaccine Strain of Yersinia pestis EV76-B-SHUΔpla and Evaluation of Its Protection Efficacy in a Mouse Model by Aerosolized Intratracheal Inoculation

Construction of a Live-Attenuated Vaccine Strain of Yersinia pestis EV76-B-SHUΔpla and Evaluation of Its Protection Efficacy in a Mouse Model by Aerosolized Intratracheal Inoculation

Development and evaluation of an up-converting phosphor technology-based lateral flow assay for rapid and quantitative detection of Coxiella burnetii phase I strains

Synthetic Particulate Subunit Vaccines for the Prevention of Q Fever

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