Enhanced production of thermostable laccases from a native strain of Pycnoporus sanguineus using central composite design

Abstract

Abstract:The production of thermostable laccases from a native strain of the white-rot fungus Pycnoporus sanguineus isolated in Mexico was enhanced by testing different media and a combination of inducers including copper sulfate (CuSO 4 ). The best conditions obtained from screening experiments in shaken flasks using tomato juice, CuSO 4 , and soybean oil were integrated in an experimental design. Enhanced levels of tomato juice as the medium, CuSO 4 and soybean oil as inducers (36.8% (v/v), 3 mmol/L, and 1% (v/v), respectively) were determined for 10 L stirred tank bioreactor runs. This combination resulted in laccase titer of 143 000 IU/L (2,2'-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid), pH 3.0), which represents the highest activity so far reported for P. sanguineus in a 10-L fermentor. Other interesting media resulting from the screening included glucose-bactopeptone which increased laccase activity up to 20 000 IU/L, whereas the inducers Acid Blue 62 and Reactive Blue 19 enhanced enzyme production in this medium 10 times. Based on a partial characterization, the laccases of this strain are especially promising in terms of thermostability (half-life of 6.1 h at 60 °C) and activity titers.

Aislamiento Y Evaluación De La Actividad Enzimática Ligninolítica De Macromicetos Del Estado De Veracruz, México

“…Las máximas actividades para lacasa y MnP fueron de 5422.2 U/mg de proteína (16 256.5 U/L) y 41.5 U/mg de proteína (146.0 U/L), respectivamente. La actividad lacasa obtenida por P. sanguineus ACT1 es ligeramente menor a la actividad reportada por Ramírez-Cavazos et al (2014) con una cepa nativa del noreste de México, la cual presentó 20 000 U/L en un medio enriquecido con jugo de tomate y glucosa/bactopeptona. La actividad MnP de P. sanguineus ha sido menos estudiada en comparación con la de la lacasa.…”

Stimulation of laccases from Trametes pubescens: Use in dye decolorization and cotton bleaching

“…as dyes) are present and affect the secondary metabolism. Even though the amplitude of the potential synergism among factors is difficult to define in advance, this phenomenon has been observed in several studies (6,18,20).…”

Tree bark scrape fungus: A potential source of laccase for application in bioremediation of non-textile dyes

“…For laccase production 2 plugs growth of fungus was grown in minimal medium (MM) containing (gL -1 ) glucose, 3.0; KH 2 PO 4 , 1.0, (NH 4 )2SO 4 [25] at 32 o C for 12 days. The cell-free extract obtained following the centrifugation at 10,000 rpm for 15 minutes at 4 o C was used for laccase assay with ABTS as standard (100-1000 µgmL -1 ) [26].…”