Enhanced production of novel halostable recombinant endoglucanase derived from the metagenomic library using fed-batch fermentation

Exploration of production of C14 and C15 bacillomycin D homologues with enzymatic hydrolysis from maize straws using fed-batch fermentation by Bacillus subtilis NS-174

“…It was well known that the fed-batch fermentation could signicantly improve the biomass concentration of microorganisms and the synthetic ability of target substances. 16,35 The similar evidence was also found by Zhong et al (2014), which signicantly increased the production of an antifungal cyclic lipopeptide (jiean-peptide) by Bacillus subtilis using fed-batch fermentation. 36 In the present study, according to the evidences of Fig.…”

“…14 In addition, the fed-batch fermentation was widely used in bio-fermentation because of its rapid accumulation of biomass and shortening fermentation cycle. 15,16 Use of enzymatic hydrolysates from crop straws as fermentation substrates and optimization fermentation strategies could ulteriorly improve the biomass of microorganisms and the synthesis efficiency of target substances. [17][18][19][20] However, at present, there were no reports on production of antifungal lipopeptides from microorganisms using enzymatic hydrolysis of crop straws.…”

Biochemical characterization of a novel thermo‐halo‐tolerant GH5 endoglucanase from a thermal spring metagenome

“…Many fungal and bacterial strains have been characterized for endoglucanase production; however, enzymes of bacterial origin could offer some advantages associated with its ability to thrive in harsh environments, relatively faster growth, and a better yield of the recombinant enzyme. Some of the recently reported GH5 endoglucanases are from Bacillus subtilis 1AJ3 (Ma et al, 2020), Thermoactinospora rubra YIM 77501T (Yin et al, 2020), Cellulomonas bogoriensis 69B4T (Li et al, 2020), Thermobifida alba AHK119 (Ohta et al, 2019), Aspergillus glaucus CCHA (Li et al, 2018), Saccharophagus degradans 2‐40T (Wang et al, 2016), Jonesia quinghaiensis (Lin et al, 2016), cow rumen metagenome (Patel et al, 2020), termite gut metagenome (Guerrero et al, 2020), porcine gut metagenome (Wang et al, 2019), rhizosphere metagenome (Wierzbicka‐Wos et al, 2019), soil metagenome (Kumar et al, 2019; Pimentel et al, 2017), hot spring metagenome (Gupta et al, 2017; Zarafeta et al, 2016), and so forth.…”

Role of metagenomics in prospecting novel endoglucanases, accentuating functional metagenomics approach in second-generation biofuel production: a review

“…This modification might sometimes increase or decrease the activity of the enzyme [192]. Cel5R reported by Narender Kumar et al [173] has enhanced its activity in the presence of methanol, and in most of the reported cases, it has partially reduced the enzyme's activity whereas in the case of Cel5G reported by Juan Liu et al [149] the activity was completely inhibited. In 2011, Soyoun Hwang et al reported that the presence of methanol expands the structure of the protein, probably by reducing protein's hydrophobic properties.…”

“…Most of the reported enzymes had reducing effects on the enzyme's activity in the presence of acetone, and surprisingly in the case of Cel5R reported by Narender Kumar et al, acetone has enhanced its activity [173]. Acetone is generally used for precipitating protein and sometimes for concentrating them also.…”