Enhanced Production of Mineralized Nodules and Collagenous Proteins In Vitro by Calcium Ascorbate Supplemented With Vitamin C Metabolites

Rowe, Dorothy J.; Ko, Susan; Tom, Xenia M.; Silverstein, Steven J.; Richards, David

doi:10.1902/jop.1999.70.9.992

Cited by 20 publications

(10 citation statements)

References 35 publications

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“…It is also well known that vitamin C is a marker for osteoblast formation and has been shown to stimulate procollagen and enhance collagen synthesis [11][12][13][14] . Therefore, L-threonic acid may play a role in the mineralization process through its positive action on vitamin C. This hypothesis was confirmed in 1999 by Rowe DJ [15] . It was reported that in vitro treatment with ascorbatecontaining vitamin C metabolites enhanced the formation of the mineralized nodules and collagenous proteins and that L-threonate was one of the metabolites that was found to influence the mineralization process [15] .…”

Section: Introductionmentioning

confidence: 79%

“…Therefore, L-threonic acid may play a role in the mineralization process through its positive action on vitamin C. This hypothesis was confirmed in 1999 by Rowe DJ [15] . It was reported that in vitro treatment with ascorbatecontaining vitamin C metabolites enhanced the formation of the mineralized nodules and collagenous proteins and that L-threonate was one of the metabolites that was found to influence the mineralization process [15] . Recently, a preclinical study was performed to investigate the effect of L-threonate on bone resorption of rabbit osteoclasts [16] .…”

Section: Introductionmentioning

confidence: 79%

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Pharmacokinetics and safety of calcium L-threonate in healthy volunteers after single and multiple oral administrations

Wang

Jiang

2011

Acta Pharmacol Sin

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Aim:To evaluate the pharmacokinetics of L-threonate after single or multiple oral administrations and its safety profile in healthy Chinese volunteers. Methods:This was an open-label, single-and multiple-dose study. The subjects were assigned to receive a single dose, 675, 2025, or 4050 mg, of calcium L-threonate (n=12) or repeated doses of 2025 mg twice daily for 4 d (n=12). Serial plasma and urine samples were analyzed with HPLC-MS/MS. Pharmacokinetic parameters of L-threonate were calculated using non-compartmental analysis with WinNonlin software. Results: In the single dose group, C max reached at 2.0 h and the mean t 1/2 was approximately 2.5 h. Area under curve (AUC) and C max increased with dose escalation, but dose proportionality was not observed over the range of 675 to 4050 mg. AUC and C max in the fasted subjects were lower compared with those in the non-fasted subjects. Cumulative urinary excretion of L-threonate over 24 h represented 5.9% of the administered dose with a mean Cl/r of 0.8 L/h. In the multiple-dose study, no accumulation appeared upon repeated doses of 2025 mg twice daily for 4 d. There were no serious adverse events that occurred during this study. Conclusion: Calcium L-threonate was well tolerated in healthy Chinese subjects, with no pattern of dose-related adverse events. Plasma exposure increased with dose escalation, but linear pharmacokinetics were not observed over the studied doses. L-threonate was absorbed rapidly, and its absorption was enhanced by food intake. No systemic accumulation appeared after repeated administrations.

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Section: Introductionmentioning

confidence: 79%

Section: Introductionmentioning

confidence: 79%

Pharmacokinetics and safety of calcium L-threonate in healthy volunteers after single and multiple oral administrations

Wang

Jiang

2011

Acta Pharmacol Sin

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“…However, current data do not clarify if reduced inhibition of STS via lower deoxycholate in women with high FV intake directly leads to greater STS production and/or activity (Supplemental Table 8 61 . Lower threonate in OS women could relate to tissue mineralization and bone resorption 62,63 . Lower homoarginine is an indicator of higher bone for use under a CC0 license.…”

Section: Discussionmentioning

confidence: 99%

Diet-derived fruit and vegetable metabolites suggest sex-specific mechanisms conferring protection against osteoporosis in humans

Mangano

Noël

Lai

et al. 2019

Preprint

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The impact of nutrition on the metabolic profile of osteoporosis is incompletely characterized. The objective of this cross-sectional study was to detangle the association of fruit and vegetable (FV) intakes with osteoporosis prevalence. Dietary, anthropometric and blood plasma metabolite data were examined from the Boston Puerto Rican Osteoporosis Study, a cohort of 600 individuals (age 46-79yr). High FV intake was protective against osteoporosis prevalence (Odds Ratio=0.73; 95% CI = 0.57, 0.94; P=0.013). Associations of 525 plasma metabolites were assessed with fruit and vegetable intake, and separately with osteoporosis status. Several biological processes affiliated with the FV-associating metabolites, including caffeine metabolism, carnitines and fatty acids, and glycerophospholipids. For osteoporosis-associated metabolites, important processes were steroid hormone biosynthesis in women, and branched-chain amino acid metabolism in men. In all instances, the metabolite patterns differed greatly between sexes, arguing for a stratified nutrition approach in recommending FV intakes to improve bone health. Factors derived from principal components analysis of the FV intakes were correlated with the osteoporosis-associated metabolites, with high intake of dark leafy greens and berries/melons appearing protective in both sexes. These data warrant investigation into whether increasing intakes of dark leafy greens, berries and melons causally affect bone turnover and BMD among adults at risk for osteoporosis via sex-specific metabolic pathways, and how gene-diet interactions alter these sex-specific metabolomic-osteoporosis links.

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“…This difference may reflect variation in ascorbate turnover in lactating female and developing pups. L-Threonate can improve the proliferation of osteoblasts and enhance production of mineralized nodules and collagenous protein (Fay and Verlangieri, 1991; Rowe et al, 1999). This may be indicative of bone formation and growth in the weaned pups over their developmental fast.…”

Section: Discussionmentioning

confidence: 99%

Water-soluble vitamin homeostasis in fasting northern elephant seals (Mirounga angustirostris) measured by metabolomics analysis and standard methods

Boaz

Champagne

Fowler

et al. 2012

Comparative Biochemistry and Physiology Part A: Molecular &

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Despite the importance of water-soluble vitamins to metabolism, there is limited knowledge of their serum availability in fasting wildlife. We evaluated changes in water-soluble vitamins in northern elephant seals, a species with an exceptional ability to withstand nutrient deprivation. We used a metabolomics approach to measure vitamins and associated metabolites under extended natural fasts for up to seven weeks in free-ranging lactating or developing seals. Water-soluble vitamins were not detected with this metabolomics platform, but could be measured with standard assays. Concentrations of measured vitamins varied independently, but all were maintained at detectable levels over extended fasts, suggesting that defense of vitamin levels is a component of fasting adaptation in the seals. Metabolomics was not ideal for generating complete vitamin profiles in this species, but gave novel insights into vitamin metabolism by detecting key related metabolites. For example, niacin level reductions in lactating females were associated with significant reductions in precursors suggesting downregulation of the niacin synthetic pathway. The ability to detect individual vitamins using metabolomics may be impacted by the large number of novel compounds detected. Modifications to the analysis platforms and compound detection algorithms used in this study may be required for improving water-soluble vitamin detection in this and other novel wildlife systems.

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Enhanced Production of Mineralized Nodules and Collagenous Proteins In Vitro by Calcium Ascorbate Supplemented With Vitamin C Metabolites

Cited by 20 publications

References 35 publications

Pharmacokinetics and safety of calcium L-threonate in healthy volunteers after single and multiple oral administrations

Pharmacokinetics and safety of calcium L-threonate in healthy volunteers after single and multiple oral administrations

Diet-derived fruit and vegetable metabolites suggest sex-specific mechanisms conferring protection against osteoporosis in humans

Water-soluble vitamin homeostasis in fasting northern elephant seals (Mirounga angustirostris) measured by metabolomics analysis and standard methods

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