Enhanced production of lipase by the thermophilic Geobacillus stearothermophilus strain-5 using statistical experimental designs

Sifour, Mohamed; Zaghloul, Taha I.; Saeed, Hesham; Berekaa, Mahmoud M.; Abdel-Fattah, Yasser R.

doi:10.1016/j.nbt.2010.04.004

Cited by 25 publications

(15 citation statements)

References 28 publications

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“…Tween-80 not only acted as an effective surfactant but also a lipase inducer [13]. Because RSM can serve as a successive and exploratory tool for establishing the interaction of variables [16,18], a set of 20 experiments designed with the faced-centered central composite design (FCCCD) were carried out to determine their optimum concentrations for maximum lipase production. The design matrix of the variables in coded units and actual concentrations along with the experimental response (lipase production) is presented in Table 1.…”

Section: Resultsmentioning

confidence: 99%

Optimization of Lipase Production by Burkholderia sp. Using Response Surface Methodology

Kuan

et al. 2012

IJMS

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Response surface methodology (RSM) was employed to optimize the extracellular lipase production by Burkholderia sp. HL-10. Preliminary tests showed that olive oil, tryptone and Tween-80 exhibited significant effects on the lipase production. The optimum concentrations of these three components were determined using a faced-centered central composite design (FCCCD). The analysis of variance revealed that the established model was significant (p < 0.01). The optimized medium containing 0.65% olive oil (v/v), 2.42% tryptone (w/v) and 0.15% Tween-80 (v/v) resulted in a maximum activity of 122.3 U/mL, about three fold higher than that in basal medium. Approximately 99% of validity of the predicted value was achieved.

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Section: Resultsmentioning

confidence: 99%

Optimization of Lipase Production by Burkholderia sp. Using Response Surface Methodology

Kuan

et al. 2012

IJMS

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“…The Plackett-Burman method is comprehensively used for the identification of significant variables as well as their significance levels [27]. From the studied nutrient variables different ingredients have different effects on neomycin production.…”

Section: Discussionmentioning

confidence: 99%

Optimization of Medium Composition for the Production of Neomycin by Streptomyces fradiae NCIM 2418 in Solid State Fermentation

Vastrad¹,

Neelagund

2014

Biotechnology Research International

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Neomycin production of Streptomyces fradiae NCIM 2418 was optimized by using response surface methodology (RSM), which is powerful mathematical approach comprehensively applied in the optimization of solid state fermentation processes. In the first step of optimization, with Placket-Burman design, ammonium chloride, sodium nitrate, L-histidine, and ammonium nitrate were established to be the crucial nutritional factors affecting neomycin production significantly. In the second step, a 24 full factorial central composite design and RSM were applied to determine the optimal concentration of significant variable. A second-order polynomial was determined by the multiple regression analysis of the experimental data. The optimum values for the important nutrients for the maximum were obtained as follows: ammonium chloride 2.00%, sodium nitrate 1.50%, L-histidine 0.250%, and ammonium nitrate 0.250% with a predicted value of maximum neomycin production of 20,000 g kg−1 dry coconut oil cake. Under the optimal condition, the practical neomycin production was 19,642 g kg−1 dry coconut oil cake. The determination coefficient (R 2) was 0.9232, which ensures an acceptable admissibility of the model.

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“…9 The bacterial medium composition greatly influenced the lipase synthesis and its activity. [10][11][12][13] The objective of the study was to determine the medium composition of Thalassospira permensis M35-15 for maximum lipase production, using statistical designs of Plackett-Burman experimental design and central composite design of Response Surface Methodology.…”

Section: Introductionmentioning

confidence: 99%

Optimization of Lipase production from a novel strainThalassospira permensisM35-15 using Response Surface Methodology

Wang

Yan

2016

Bioengineered

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Lipases can catalyze the hydrolysis of glycerol, esters and long chain fatty acids. A lipase producing isolate M35-15 was screened and identified as Thalassospira permensis using 16S rRNA gene sequence analysis. To our knowledge this is the first report on Thalassospira permensis producing lipases. In this paper the optimization of medium composition for the increase in bacterial lipase was achieved using statistical methods. Firstly the key ingredients were selected by PlackettBurman experimental design, then the levels of the ingredients were optimized using central composite design of Response Surface Methodology. The predicted optimal lipase activity was 11.49 U under the conditions that medium composition were 5.15 g/l glucose, 11.74 g/l peptone, 6.74 g/l yeast powder and 22.90 g/l olive oil emulsifier.

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Enhanced production of lipase by the thermophilic Geobacillus stearothermophilus strain-5 using statistical experimental designs

Cited by 25 publications

References 28 publications

Optimization of Lipase Production by Burkholderia sp. Using Response Surface Methodology

Optimization of Lipase Production by Burkholderia sp. Using Response Surface Methodology

Optimization of Medium Composition for the Production of Neomycin by Streptomyces fradiae NCIM 2418 in Solid State Fermentation

Optimization of Lipase production from a novel strainThalassospira permensisM35-15 using Response Surface Methodology

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