Enhanced production of l(+)-lactic acid by floc-form culture of Rhizopus oryzae

Yu, Mei‐Ching; Wang, Ron-Chi; Wang, Chung-Yih; Duan, Kow‐Jen; Sheu, Dey-Chyi

doi:10.1016/j.jcice.2007.02.005

Cited by 33 publications

(21 citation statements)

References 16 publications

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“…Rhizopus species, such as R. oryzae and R. arrhizus, have amylolytic enzymatic activity, which enables them to convert starch directly to L(+)-lactic acid. Several studies have reported on the use of Rhizopus for lactic acid production (Yin et al 1998;Liu et al 2006;Yu et al 2007;Guo et al 2010;Wu et al 2011;Saito et al 2012). Fig.…”

Section: Bioresourcescommentioning

confidence: 99%

Lactic Acid Production to Purification: A Review

Komesu

Oliveira

Martins

et al. 2017

BioRes

195

114

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Lactic acid is a naturally occurring organic acid that can be used in a wide variety of industries, such as the cosmetic, pharmaceutical, chemical, food, and, most recently, the medical industries. It can be made by the fermentation of sugars obtained from renewable resources, which means that it is an eco-friendly product that has attracted a lot of attention in recent years. In 2010, the U.S. Department of Energy issued a report that listed lactic acid as a potential building block for the future. Bearing the importance of lactic acid in mind, this review summarizes information about lactic acid properties and applications, as well as its production and purification processes.

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Section: Bioresourcescommentioning

confidence: 99%

Lactic Acid Production to Purification: A Review

Komesu

Oliveira

Martins

et al. 2017

BioRes

195

114

View full text Add to dashboard Cite

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“…As compared with traditional batch operations, the repeated-batch mode often makes a fermentation process more efficient. It has been successfully employed for the production of ethanol [18], lactic [21] and itaconic acid [10]. Repeated-batch operations for the production of citric acid by yeasts using glucose [2,12] and ethanol [3] have increasingly received research interest in the last years; however, the yields achieved were not high enough for a competitive economic industrial operation.…”

Section: Introductionmentioning

confidence: 99%

High-yield production of citric acid by Yarrowia lipolytica on glycerol in repeated-batch bioreactors

Rywińska

Rymowicz

2010

J Ind Microbiol Biotechnol

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An acetate negative mutant of Yarrowia lipolytica Wratislavia AWG7 was found to be suitable for the production of high amounts of citric acid in long-term repeated-batch cultures. When 40% of fresh replaced medium was fed, this strain produced 154 g l(-1), on average, which corresponded to a 0.78 g g(-1) yield and a productivity of 1.05 g l(-1) h(-1). The activity of the culture remained stable for more than 1,650 h, i.e., 16 cycles of the repeated-batch bioreactors.

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“…In the production formation stage, fermentation is carried out using the existing uniform pellets; with less ions and nitrogen source, higher L-lactic acid yield can be reached in shorter time. Yu et al used Xocform R. oryzae to produce L-lactic acid by semicontinuous fermentation; for the Wrst four cycles the volumetric productivity and yield were 4.03 g/(l·h) and 0.90, respectively, in 28 h, but volumetric productivity decreased to 3.44 g/ (l·h) in 32 h in cycle 5 and cycle 6 [7]. Yin et al used small mycelia pellets of R. oryzae to produce L-lactic acid for nine cycles over 14 days, and the average volumetric productivity was 2.02 g/(l·h) for the Wrst six cycles in an air-lift bioreactor [8].…”

Section: Introductionmentioning

confidence: 99%

Production of l-lactic acid by Rhizopus oryzae using semicontinuous fermentation in bioreactor

Jiang

Liu

et al. 2010

J Ind Microbiol Biotechnol

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Semicontinuous fermentation using pellets of Rhizopus oryzae has been recognized as a promising technology for L-lactic acid production. In this work, semicontinuous fermentation of R. oryzae AS 3.819 for L-lactic acid production has been developed with high L-lactic acid yield and volumetric productivity. The effects of factors such as inoculations, CaCO₃ addition time, and temperature on L-lactic acid yield and R. oryzae morphology were researched in detail. The results showed that optimal fermentation conditions for the first cycle were: inoculation with 4% spore suspension, CaCO₃ added to the culture medium at the beginning of culture, and culture temperature of 32-34 °C. In orthogonal experiments, high L-lactic acid yield was achieved when the feeding medium was (g/l): glucose, 100; (NH₄)₂SO₄, 2; KH₂PO₄, 0.1; ZnSO₄·7H₂O, 0.33; MgSO₄·7H₂O, 0.15; CaCO₃, 50. Twenty cycles of semicontinuous fermentation were carried out in flask culture. L: -lactic acid yield was 78.75% for the first cycle and 80-90% for the repeated cycles; the activities of lactate dehydrogenases (LDH) were 7.2-9.2 U/mg; fermentation was completed in 24 h for each repeated cycle. In a 7-l magnetically stirred fermentor, semicontinuous fermentation lasted for 25 cycles using pellets of R. oryzae AS 3.819 under the optimal conditions determined from flask cultures. The final L-lactic acid concentration (LLAC) reached 103.7 g/l, and the volumetric productivity was 2.16 g/(l·h) for the first cycle; in the following 19 repeated cycles, the final LLAC reached 81-95 g/l, and the volumetric productivities were 3.40-3.85 g/(l·h).

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Enhanced production of l(+)-lactic acid by floc-form culture of Rhizopus oryzae

Cited by 33 publications

References 16 publications

Lactic Acid Production to Purification: A Review

Lactic Acid Production to Purification: A Review

High-yield production of citric acid by Yarrowia lipolytica on glycerol in repeated-batch bioreactors

Production of l-lactic acid by Rhizopus oryzae using semicontinuous fermentation in bioreactor

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