Enhanced Production of Extracellular Lipase by Novel Mutant Strain of<i>Aspergillus Niger</i>

Toscano, Lydia; Gochev, Velizar; Montero, Gisela; Stoytcheva, Margarita

doi:10.5504/bbeq.2011.0019

Cited by 25 publications

(18 citation statements)

References 17 publications

(29 reference statements)

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“…Minimum activity of lipase (27.80 ± 0.42 U mL -1 min -1 ) was found after 30 minutes of incubation. The achieved impact of UV mutagenesis on activity of A. niger LPF-5 lipase was found similar to the report of Toscano et al (2011) where selected mutant of A. niger (UV 2 ) demonstrated 19% more activity of lipase (8.85 U cm -3 ) as compared to activity (7.50 U cm -3 ) of wild strain. Karanam and Medicherla (2008) reported 27% higher lipase activity (9.42 U mL -1 min -1 ) of UV mutant (AUV 3 ) strain compared to parent strain (7.44 U mL -1 min -1 ) of A. japonicus MTCC 1975.…”

Section: Physical Mutagenesis By Uv Light and Selection Of The Potentsupporting

confidence: 72%

“…In last few years, efforts have been made for the excess production of microbial enzymes by induced mutagenesis (Ravindranath and Lakshmi, 2014). Toscano et al (2011) reported that the extracellular production of lipase was increased by a mutant derivative of A. niger as compared to parent strain. This mutant strain was obtained by UV light.…”

Section: Introductionmentioning

confidence: 99%

“…Hence, they have applications in several areas of biotechnology. Lipases are broadly used for hydrolysis, biotransformations, synthesis as well as separations in different industries such as paper, chemical, waste water treatment, flavor and food industries, and in medical diagnostic, scientific researches, chemical analysis and therapeutics (Toscano et al, 2011). Among the lipolytic microorganisms, filamentous fungi associated with the 146 diverse species of genera Trichoderma, Rhizopus, Aspergillus and Penicillium were reported as the potent lipase producers (Musa and Adebayo-Tayo, 2012).…”

Section: Introductionmentioning

confidence: 99%

“…But only some considered the possibilities for increasing the enzyme production by strain improvement (Bapiraju et al, 2004;Toscano et al, 2011). As the amount of the enzymes secreted by wild strains is low, therefore overproduction of the enzymes requires improvement of strain and optimization of culture medium.…”

Section: Introductionmentioning

confidence: 99%

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Enhancement of Extracellular Lipase Production by Strain Improvement of Fungus Aspergillus niger LPF-5

Sharma¹,

Sharma²,

Saxena³

2016

IJSRES

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Abstract. Twelve fungal isolates belonging to different genera were quantitatively screened for extracellular lipase production in SmF (Submerged fermentation). Isolate LPF-5 demonstrated higher lipase activity and it was identified as Aspergillus niger based on morphology of its Petri plate culture, microscopic study of its slide culture and with the help of relevant literatures. Further an effort was made to increase the production of extracellular lipase by subjecting the most potent lipolytic fungal strain A. niger LPF-5 to the strain improvement by induced mutagenesis using UV radiations and nitrous acid. Among the all tested mutagens, nitrous acid was found to be the best mutagen (incubation time of 15 minutes) for inducing the favorable mutation in fungal strain A. niger LPF-5 which enhanced the lipase activity up to 30% (105.19 ± 0.91 U mL -1 min -1) when compared to lipase activity (81.00 ± 0.30 U mL -1 min -1 ) of wild strain while the lipase activity of the best UV mutant (A. niger UV3), obtained after an incubation of 6 minutes was 94.30 ± 0.54 U mL -1 min -1 , which was 16.41% higher as compared to wild strain of A. niger. These results indicate that UV light and nitrous acid both were effective mutagens but nitrous acid was more potent mutagen than UV light.

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Section: Physical Mutagenesis By Uv Light and Selection Of The Potentsupporting

confidence: 72%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Enhancement of Extracellular Lipase Production by Strain Improvement of Fungus Aspergillus niger LPF-5

Sharma¹,

Sharma²,

Saxena³

2016

IJSRES

View full text Add to dashboard Cite

show abstract

“…Subsequently, there have been developed research projects on topics related to the inventory of residual feedstocks to produce biodiesel, the performance of internal combustion engines operating with diesel-biodiesel blends, as well as the study of the behavior of materials exposed to diesel, biodiesel and mixtures of both [6,15,16,[19][20][21]. Research focused on the production of biodiesel via enzymatic has been conducted as well [22][23][24][25].…”

Section: Non-industrial Experiences Of Biodiesel Production In Mexicomentioning

confidence: 99%