Enhanced production of erythritol by Yarrowia lipolytica on glycerol in repeated batch cultures

Abstract

Erythritol is an important natural sweetener, industrially produced only by fermentation on glucose media. Glycerol is an important renewable feedstock as it is the major by-product of the biodiesel production process; here we present an alternative way to convert this low-cost substrate into value-added products, such as erythritol. Repeated batch cultures (RBC) were performed to improve the productivity of erythritol from pure and crude glycerol. An acetate negative mutant of Yarrowia lipolytica Wratislavia K1 was found to be applicable for the production of high amounts of erythritol in RBC. When 20 % of fresh replaced medium was added, the strain Wratislavia K1 was able to produce 220 g l −1 erythritol, which corresponded to a 0.43 g g−1 yield and a productivity of 0.54 g l−1 h−1. Additionally, the activity of the culture remained stable for more than 1,000 h, i.e., 11 cycles of the repeated batch bioreactors.

Biotechnological applications of Yarrowia lipolytica: Past, present and future

“…Naturally, erythritol productivity is affected by various factors, such as the culture mode, agitation rate and osmotic pressure (Tomaszewska et al, 2012;Rywińska et al, 2013bRywińska et al, , 2015Tomaszewska et al, 2014). Mirończuk et al (2014) developed the fed-batch culture mode for erythritol production. Under optimized conditions, up to 220 g/L erythritol was produced by mutant Y. lipolytica Wratislavia K1.…”

A two-stage fermentation process of erythritol production by yeast Y. lipolytica from molasses and glycerol

“…Y. lipolytica was classified as a GRAS (generally recognizes as safe) microorganism and therefore can easily be used in industry (Groenewald et al, 2014). It was shown that Y. lipolytica can convert pure or crude glycerol into organic acids, polyols, single cell oils (SCO), and single cell proteins (SCP) in different cultivation systems (Dobrowolski et al, 2013;Kamzolova et al, 2015;Lazar et al, 2014;Miroń czuk et al, 2014;Rywiń ska et al, 2013;Rymowicz et al, 2010).…”

Physiologo-biochemical characteristics of citrate-producing yeast Yarrowia lipolytica grown on glycerol-containing waste of biodiesel industry

“…Glycerol-based microbiological processes have been developed which aimed at production of 1,3-propanediol (Papanikolaou et al 2008), poly-3-hydroxybutyrate (Mothes et al 2007;Ibrahim and Steinbüchel 2010), lipids (Papanikolaou and Aggelis 2010;Papanikolaou and Aggelis 2002;Dedyukhina et al 2012), polyols (Rywińska et al 2013;Mirończuk et al 2014), arachidonic acid (Dedyukhina et al 2014), pyruvic acid (Morgunov et al 2004a), succinic acid (Yuzbashev et al 2010;Jost et al 2014), α-ketoglutaric acid Yin et al 2012;Otto et al 2012), and citric acid (Rymowicz et al 2006;Förster et al 2007a;Levinson et al 2007;Rymowicz et al 2006Rymowicz et al , 2010Holz et al 2009;Kamzolova et al 2011a;Rywińska et al 2009Rywińska et al , 2013Morgunov et al 2013). Citric acid (CA) is considered to be the most economically feasible product with the annual world production of 1.4 million ton (Anastassiadis et al 2008).…”