Enhanced production of erythritol and mannitol by Yarrowia lipolytica in media containing surfactants

Rakicka, Magdalena; Rywińska, Anita; Cybulski, Krzysztof; Rymowicz, Waldemar

doi:10.1016/j.bjm.2016.01.011

Cited by 46 publications

(27 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The process conducted for the control strain at low agitation was inefficient and time consuming compares with the previous studies [5, 21, 32]. These data confirm that oxygen limitation has a huge impact on erythritol synthesis by Y. lipolytica .…”

Section: Resultssupporting

confidence: 79%

Functional overexpression of genes involved in erythritol synthesis in the yeast Yarrowia lipolytica

Mirończuk

Biegalska

Dobrowolski

2017

Biotechnol Biofuels

View full text Add to dashboard Cite

BackgroundErythritol, a four-carbon polyol synthesized by microorganisms as an osmoprotectant, is a natural sweetener produced on an industrial scale for decades. Despite the fact that the yeast Yarrowia lipolytica has been reported since the 1970s as an erythritol producer, the metabolic pathway of this polyol has never been characterized. It was shown that erythritol synthesis in yeast occurs via the pentose phosphate pathway (PPP). The oleaginous yeast Y. lipolytica is a good host for converting inexpensive glycerol into a value-added product such as erythritol. Glycerol is a renewable feedstock which is produced on a large scale as a waste product by many branches of industry.ResultsIn this study, we functionally overexpressed four genes involved in the pentose phosphate pathway (PPP): gene YALI0E06479g encoding transketolase (TKL1), gene YALI0F15587g encoding transaldolase (TAL1), gene YALI0E22649g encoding glucose-6-phosphate dehydrogenase (ZWF1), and gene YALI0B15598g encoding 6-phosphogluconate dehydrogenase (GND1). Here, we show that the crucial gene for erythritol synthesis in Y. lipolytica is transketolase. Overexpression of this gene results in a twofold improvement in erythritol synthesis during a shake-flask experiment (58 g/L). Moreover, overexpression of TKL1 allows for efficient production of erythritol independently from the supplied dissolved oxygen. Fermentation conducted in a 5-L bioreactor at low agitation results in almost 70% higher titer of erythritol over the control strain.ConclusionThis work presents the importance of the PPP in erythritol synthesis and the feasibility for economic production of erythritol from glycerol by the yeast Y. lipolytica.Electronic supplementary materialThe online version of this article (doi:10.1186/s13068-017-0772-6) contains supplementary material, which is available to authorized users.

show abstract

Section: Resultssupporting

confidence: 79%

Functional overexpression of genes involved in erythritol synthesis in the yeast Yarrowia lipolytica

Mirończuk

Biegalska

Dobrowolski

2017

Biotechnol Biofuels

View full text Add to dashboard Cite

show abstract

“…The fact itself of the almost exclusive production of polyols (with very small side production of citric acid) in nitrogen‐limited Gly‐based media in which medium pH was always >5·0 by Y. lipolytica strains is a rather original result and has been reported yet only in a limited number of studies (Egermeier et al ; Papanikolaou et al ). In contrast, in most papers in the literature, (almost) exclusive biosynthesis and accumulation into the medium of polyols by Y. lipolytica strains has been performed in trials with medium pH <4·0 (mostly ≈3·5) (Rymowicz et al ; Tomaszewska et al ; Rywińska et al ; Mirończuk et al ; Tomaszewska‐Hetman and Rywińska ; Rakicka et al , b). Moreover, the almost exclusive biosynthesis of mannitol vs the other polyols reported for the Y. lipolytica strains tested in the current investigation (mannitol selectivity 80–88% w/w of total polyols; see Fig.…”

Section: Discussionmentioning

confidence: 99%

“…Industrial Gly has been used as the sole carbon and energy source from various yeast and fungal in order for SCO to be produced (Chatzifragkou et al ; Dedyukhina et al ; Carota et al ; Carsanba et al ). Likewise, Gly has been converted into polyols (mostly mannitol, erythritol and arabitol (Ara)) by strains of the species Yarrowia lipolytica (Tomaszewska et al ; Mirończuk et al ; Rakicka et al , b, ; Papanikolaou et al ) and, in scarce studies, into (mostly) Ara by osmophilic yeast species such as the ones of the genus Debaryomyces (Koganti et al ; Koganti and Ju ).…”

Section: Introductionmentioning

confidence: 99%

Isolation, identification and screening of yeasts towards their ability to assimilate biodiesel‐derived crude glycerol: microbial production of polyols, endopolysaccharides and lipid

et al. 2019

View full text Add to dashboard Cite

Aims To assess the ability of various newly isolated or belonging in official collections yeast strains to convert biodiesel‐derived glycerol (Gly) into added‐value compounds. Methods and Results Ten newly isolated yeast strains belonging to Debaryomyces sp., Naganishia uzbekistanensis, Rhodotorula sp. and Yarrowia lipolytica, isolated from fishes, metabolized Gly under nitrogen limitation. The aim of the study was to identify potential newly isolated microbial candidates that could produce single‐cell oil (SCO), endopolysaccharides and polyols when these micro‐organisms were grown on biodiesel‐derived Gly. As controls producing SCO and endopolysaccharides were the strains Rhodotorula glutinis NRRL YB‐252 and Cryptococcus curvatus NRRL Y‐1511. At initial Gly (Gly0) ≈40 g l−1, most strains presented remarkable dry cell weight (DCW) production, whereas Y. lipolytica and Debaryomyces sp. produced non‐negligible quantities of mannitol and arabitol (Ara). Five strains were further cultivated at increasing Gly0 concentrations. Rhodotorula glutinis NRRL YB‐252 produced 7·2 g l−1 of lipid (lipid in DCW value ≈38% w/w), whereas Debaryomyces sp. FMCC Y69 in batch‐bioreactor experiment with Gly0 ≈80 g l−1, produced 30–33 g l−1 of DCW and ~30 g l−1 of Ara. At shake‐flasks with Gly0 ≈125 g l−1, Ara of ~48 g l−1 (conversion yield of polyol on Gly consumed ≈0·62 g g−1) was achieved. Cellular lipids of all yeasts contained in variable concentrations oleic, palmitic, stearic and linoleic acids. Conclusions Newly isolated, food‐derived and non‐previously studied yeast isolates converted biodiesel‐derived Gly into several added‐value metabolites. Significance and Impact of the Study Alternative ways of crude Gly valorization through yeast fermentations were provided and added‐value compounds were synthesized.

show abstract

“…Strains derived from Y. lipolytica A‐101, such as Y4779, are considered to be good producers of citric acid and polyhydroxy alcohols. This particular shared phenotype is consistent: it is observed when glucose is used as well as when cheap waste materials such as glycerol are used (Rakicka et al ., ; Rymowicz et al ., , ; Tomaszewska et al ., ; Wojtatowicz et al ., ). The results of this study confirm that Y4779 is also good at producing citric acid and polyhydroxy alcohols from inulin.…”

Section: Resultsmentioning

confidence: 98%

Transforming sugars into fat - lipid biosynthesis using different sugars inYarrowia lipolytica

Hapeta

Rakicka

Dulermo

et al. 2017

Yeast

Self Cite

View full text Add to dashboard Cite

In an era of ever-increasing energy demands, a promising technology is being developed: the use of oleaginous microorganisms such as Yarrowia lipolytica to convert waste materials into biofuels. Here, we constructed two Y. lipolytica strains that displayed both increased lipid accumulation and more efficient use of biomass-derived sugars, including glucose, fructose, galactose and inulin. The first strain, Y. lipolytica YLZ150, was derived from the French wild-type strain W29. It had inhibited triacylglycerol mobilization (∆tgl4) and β-oxidation (∆pox1-6), and it overexpressed GPD1, DGA2, HXK1, the native Leloir pathway, SUC2 from Saccharomyces cerevisiae and INU1 from Kluyveromyces marxianus. The second strain, Y. lipolytica Y4779, was derived from the Polish A-101 strain. It had inhibited β-oxidation (∆mfe2) and overexpressed GPD1, DGA1, HXK1, YHT3, SUC2 and INU1. In the first experiment, strain YLZ150 was batch-cultured in media containing different hexoses; the highest values for lipid concentration and yield of lipids from the substrate were obtained using fructose (20.3 g dm and 0.14 g g , respectively). In the second experiment, we grew the two strains in fed-batch cultures to examine lipid biosynthesis from inulin (a fructose polymer). For Y4779, the lipid concentration was 10.3 g dm and the yield of lipids from substrate was 0.07 g g ; in contrast, for YLZ150, these values were 24 g dm and 0.16 g g , respectively. The YLZ150 strain is thus able to efficiently exploit glucose, fructose, galactose, sucrose and inulin for lipid biosynthesis. Copyright © 2017 John Wiley & Sons, Ltd.

show abstract

Enhanced production of erythritol and mannitol by Yarrowia lipolytica in media containing surfactants

Cited by 46 publications

References 39 publications

Functional overexpression of genes involved in erythritol synthesis in the yeast Yarrowia lipolytica

Functional overexpression of genes involved in erythritol synthesis in the yeast Yarrowia lipolytica

Isolation, identification and screening of yeasts towards their ability to assimilate biodiesel‐derived crude glycerol: microbial production of polyols, endopolysaccharides and lipid

Transforming sugars into fat - lipid biosynthesis using different sugars inYarrowia lipolytica

Contact Info

Product

Resources

About