Enhanced production of chitin deacetylase by Penicillium oxalicum SAEM-51 through response surface optimization of fermentation conditions

Pareek, Nidhi; Ghosh, Sanjoy; Singh, Rajesh; Vivekanand, Vivekanand

doi:10.1007/s13205-013-0118-4

Cited by 5 publications

(2 citation statements)

References 27 publications

(27 reference statements)

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“…Statistical optimization of fibrinolytic enzyme production was attempted by various researchers ( Liu et al, 2005 ; Deepak et al, 2008 ; Mukherjee and Rai, 2011 ; Vijayaraghavan and Vincent, 2015 ). RSM had been successfully employed for the enhanced production of chitin deacetylase ( Pareek et al, 2014 ), alkaline protease ( Puri et al, 2002 ), L -glutaminase ( Singh et al, 2013 ), lipase ( Ruchi et al, 2008 ), esterase ( Ren et al, 2006 ), and keratinase ( Paul et al, 2014 ). However, very little studies were reported to use RSM to optimize the fibrinolytic enzyme production in SSF.…”

Section: Discussionmentioning

confidence: 99%

Cow Dung Is a Novel Feedstock for Fibrinolytic Enzyme Production from Newly Isolated Bacillus sp. IND7 and Its Application in In Vitro Clot Lysis

et al. 2016

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Bacterial fibrinolytic enzymes find great applications to treat and prevent cardiovascular diseases. The novel fibrinolytic enzymes from food grade organisms are useful for thrombolytic therapy. This study reports fibrinolytic enzyme production by Bacillus sp. IND7 in solid-state fermentation (SSF). In this study, cow dung was used as the cheap substrate for the production of fibrinolytic enzyme. Enzyme production was primarily improved by optimizing the nutrient and physical factors by one-variable-at-a-time approach. A statistical method (two-level full factorial design) was applied to investigate the significant variables. Of the different variables, pH, starch, and beef extract significantly influenced on the production of fibrinolytic enzyme (p < 0.05). The optimum levels of these significant factors were further investigated using response surface methodology. The optimum conditions for enhanced fibrinolytic enzyme production were 1.23% (w/w) starch and 0.3% (w/w) beef extract with initial medium pH 9.0. Under the optimized conditions, cow dung substrate yielded 8,345 U/g substrate, and an overall 2.5-fold improvement in fibrinolytic enzyme production was achieved due to its optimization. This is the first report of fibrinolytic enzyme production using cow dung substrate from Bacillus sp. in SSF. The crude enzyme displayed potent activity on zymography and digested goat blood clot completely in in vitro condition.

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Section: Discussionmentioning

confidence: 99%

Cow Dung Is a Novel Feedstock for Fibrinolytic Enzyme Production from Newly Isolated Bacillus sp. IND7 and Its Application in In Vitro Clot Lysis

et al. 2016

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“…7 and 8) possibly due to production and secretion of hydrolytic enzymes by the fungus. Pareek et al (2014) also reported enhanced production of chitin deacetylase by Penicillium oxalicum SAE M -51 through response surface optimization of fermentation conditions. The network of fungus around the dull orange coloured egg laid on the surface of cotton leaf was also observed (Fig.…”

Section: Fig 15 Scanning Electron Micrograph Of Dead and Decayed Camentioning

confidence: 95%

Penicillum oxalicum spg1: A novel entomopathogenic fungus isolated from mummified Bemisia tabaci (Gennadius) of cotton

Kataria

Singh

Pandove

et al. 2018

JANS

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Whitefly has assumed the status of a serious pest of cotton in north India in recent past due to its severe attack on cotton crop. The productivity of cotton crop has fallen substantially from 574 kg ha -1 of lint in the year 2014 -15 to 197 kg ha -1 in the year 2015-16 in Punjab. During extensive epidemiological surveillance of whitefly on cotton crop in the month of September 2016, mummified whiteflies with fungus were noticed on both abaxial and adaxial surfaces of leaves of the infested cotton plant at village, Mandi Khurd, Mansa District of Punjab, India and at Punjab Agricultural University, Regional Research Station, Bathinda, India. The fungus was isolated and purified from the mummified white fly and characterized at molecular level by sequencing 633 bp D2 region of Large Subunit (LSU) rRNA gene and identified as Penicillium oxalicum spg1. The gene sequence has been submitted to NCBI, USA with accession no. KY214238. The microscopic studies (stereo-and scanning electron) of the cotton leaves with mummified whitefly further strengthen the entomopathogenic potential of P. oxalicum spg1. There are few reports of entomopathogenic potential of P. oxalicum spg1 against other insects and pests. To our knowledge, this is the first report of isolation and identification of P. oxalicum spg1 from mummified white fly. It's potential as a biocontrol agent against white fly can be exploited in a promising way with nominal interference with biological equilibrium.

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Nanochitosan derived from fungal and aquatic sources

Abimbola¹,

Olotu²,

Alabetutu³

et al. 2023

Next Generation Nanochitosan

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Enhanced production of chitin deacetylase by Penicillium oxalicum SAEM-51 through response surface optimization of fermentation conditions

Cited by 5 publications

References 27 publications

Cow Dung Is a Novel Feedstock for Fibrinolytic Enzyme Production from Newly Isolated Bacillus sp. IND7 and Its Application in In Vitro Clot Lysis

Cow Dung Is a Novel Feedstock for Fibrinolytic Enzyme Production from Newly Isolated Bacillus sp. IND7 and Its Application in In Vitro Clot Lysis

Penicillum oxalicum spg1: A novel entomopathogenic fungus isolated from mummified Bemisia tabaci (Gennadius) of cotton

Nanochitosan derived from fungal and aquatic sources

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