Enhanced production of butanol and xylosaccharides from Eucalyptus grandis wood using steam explosion in a semi-continuous pre-pilot reactor

Cebreiros, Florencia; Risso, Florencia; Cagno, Matías; Cabrera, María Noel; Rochón, Eloísa; Jauregui, Guillermo; Boix, Elzeario; Böthig, Silvia; Ferrari, Mario Daniel; Lareo, Claudia

doi:10.1016/j.fuel.2020.119818

Cited by 26 publications

(6 citation statements)

References 46 publications

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“…Particularly, valorization of biomass from forestry industries, such as Eucalyptus wood from several species, has been gaining attention for XOS production (Vázquez et al, 2005;Penín et al, 2019b;Cebreiros et al, 2020;Neiva et al, 2020;Neto et al, 2020) due to certain advantages of these materials in comparison with agro-industrial by-products, such as a non-seasonal character, large availability, and their ability to grow in non-food cultivable lands (Penín et al, 2020). The particular interest of the species E. nitens as raw material for XOS production, in comparison with other species such as Eucalyptus globulus, resides in the following key aspects: (i) E. nitens is a fast growing species that, unlike E. globulus, is able to grow in frosting zones (Penín et al, 2019a); (ii) it is more resistant than E. globulus to certain plagues such as Gonipterus scutellatus (Pérez et al, 2006); and (iii) the physical, chemical, and structural properties of E. nitens limit its use in pulp and paper industries, so the development of valorization alternatives for the huge amounts of wood produced is of great interest.…”

Section: Abbreviationsmentioning

confidence: 99%

Manufacture and Prebiotic Potential of Xylooligosaccharides Derived From Eucalyptus nitens Wood

et al. 2021

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Mixtures of xylooligosaccharides (XOS) were manufactured from Eucalyptus nitens samples by hydrothermal processing. In order to obtain a product suitable to be used as a prebiotic, the liquors obtained were subjected to a refining sequence consisting of a two-step membrane filtration followed by anion exchange and freeze-drying. The process proposed allowed to obtain a highly refined product mainly made up of a mixture of substituted XOS with a degree of polymerization, DP3–10, which was evaluated for its prebiotic potential by in vitro fermentation assays. Their effects on the microbiota composition and the metabolic activity were assessed along the fermentation time and compared to fructooligosaccharides (FOS, a gold standard prebiotic), using fecal inocula from donors belonging to two age-groups (young and elderly). Significant and similar increases were observed in most of the bacterial groups considered (including Bifidobacterium spp. or several butyrate-producers) in both XOS and FOS in vitro interventions, although XOS resulted in significantly higher increases in total bacteria and lower rises in Clostridium clusters I and II than FOS. Regarding the metabolic activity, higher amounts of total organic acid (TOA; 150 vs. 110 mM) and higher total short-chain fatty acid (SCFA)/TOA ratio (0.88 vs. 0.70 mol/mol) were achieved at 28 h using XOS as a carbon source in comparison with FOS. Moreover, both substrates resulted in different metabolite profiles. Higher percentages of acetate and propionate were achieved when XOS were used as substrates, whereas FOS resulted in slightly higher concentrations of butyrate. No differences were found between both age-groups. Taking together these results, it can be concluded that XOS produced from E. nitens by a biorefinery-based approach led to, at least, similar prebiotic activity as that observed with FOS.

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Section: Abbreviationsmentioning

confidence: 99%

Manufacture and Prebiotic Potential of Xylooligosaccharides Derived From Eucalyptus nitens Wood

et al. 2021

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“…Glucan recovery in the pretreatment solids was between 90 and 100%, with an average of 97%, while xylan recovery was between 11 and 50%, with an average of 29% (Figure 1). Cebreiros et al [45] reported a glucan recovery between 78 and 89% working with E. grandis sawdust at S 0 values ranging from 3.36 to 3.94, while Romaní et al [44] observed that glucan losses increased significantly for S 0 > 4.80.…”

Section: Pretreatment Of Eucalypt Sawdustmentioning

confidence: 99%

“…The lignin content in pretreated solids varied from 33.0 to 43.7%, which corresponds to lignin recoveries higher than 100% (surplus between 2 and 25% depending on the pretreatment condition). This apparent lignin overestimation is due to the accumulation of humins and/or pseudolignin that form at high pretreatment severities and subsequently quantified as acid-insoluble lignin [6,45], a feature that NREL protocols are not able to distinguish.…”

Section: Pretreatment Of Eucalypt Sawdustmentioning

confidence: 99%

Steam Explosion of Eucalypt Sawdust for Ethanol Production within a Biorefinery Approach

Guigou¹,

Guarino²,

Chiarello³

et al. 2023

Preprint

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The generation of eucalypt sawdust has increased in Uruguay with the expansion of pulp and paper industrial sites. Currently, most of this readily available feedstock is either not used or applied for producing heat and power by combustion in biomass boilers. In this work, eucalypt sawdust was subjected to steam explosion as the first step for cellulosic ethanol production within the biorefinery concept. The effect of sawdust moisture content, pretreatment temperature and residence time were evaluated, along with the influence of water washing of steam-exploded solids on enzymatic hydrolysis and fermentation yields. After steam explosion, 60% xylans and 97% acetyl groups were recovered mainly as oligomers and acetic acid in the pretreatment liquor. Eucalypt sawdust moisture content affected the enzymatic hydrolysis performance and glucan conversion efficiency. Water washing after pretreatment improved hydrolysis efficiencies and ethanol production yields by 10% with respect to the unwashed pretreated solids at the same experimental condition. The best ethanol yields were obtained using PSSF (pre-saccharification and simultaneous saccharification and fermentation) after pretreatment at 205°C for 10 min, using sawdust with an 8% moisture content. In this case, 227 L ethanol and 40 kg total xylose (including xylo-oligomers) were obtained per ton of dry eucalypt sawdust.

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“…An ideal pre-treatment method should be simple, quick, and energy-efficient, with a high enzymatic hydrolysis yield. Chemical treatments based on acetic acid, steam explosion [ 101 , 102 ], dilute sulfuric acid, and alkaline peroxide [ 103 ] have been reported to be commonly used. According to Cebreiros et al [ 101 ], steam explosion of Eucalyptus grandis biomass resulted in approximately 80% glucan enzymatic hydrolysis from pre-treated solids to fermentable sugars.…”

Section: Schemes For Obtaining Lignocellulose-based Productsmentioning

confidence: 99%

Engineered yeasts and lignocellulosic biomaterials: shaping a new dimension for biorefinery and global bioeconomy

Asemoloye,

Bello,

Oladoye

et al. 2023

Bioengineered

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The next milestone of synthetic biology research relies on the development of customized microbes for specific industrial purposes. Metabolic pathways of an organism, for example, depict its chemical repertoire and its genetic makeup. If genes controlling such pathways can be identified, scientists can decide to enhance or rewrite them for different purposes depending on the organism and the desired metabolites. The lignocellulosic biorefinery has achieved good progress over the past few years with potential impact on global bioeconomy. This principle aims to produce different bio-based products like biochemical(s) or biofuel(s) from plant biomass under microbial actions. Meanwhile, yeasts have proven very useful for different biotechnological applications. Hence, their potentials in genetic/metabolic engineering can be fully explored for lignocellulosic biorefineries. For instance, the secretion of enzymes above the natural limit (aided by genetic engineering) would speed-up the down-line processes in lignocellulosic biorefineries and the cost. Thus, the next milestone would greatly require the development of synthetic yeasts with much more efficient metabolic capacities to achieve basic requirements for particular biorefinery. This review gave comprehensive overview of lignocellulosic biomaterials and their importance in bioeconomy. Many researchers have demonstrated the engineering of several ligninolytic enzymes in heterologous yeast hosts. However, there are still many factors needing to be well understood like the secretion time, titter value, thermal stability, pH tolerance, and reactivity of the recombinant enzymes. Here, we give a detailed account of the potentials of engineered yeasts being discussed, as well as the constraints associated with their development and applications.

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Enhanced production of butanol and xylosaccharides from Eucalyptus grandis wood using steam explosion in a semi-continuous pre-pilot reactor

Cited by 26 publications

References 46 publications

Manufacture and Prebiotic Potential of Xylooligosaccharides Derived From Eucalyptus nitens Wood

Manufacture and Prebiotic Potential of Xylooligosaccharides Derived From Eucalyptus nitens Wood

Steam Explosion of Eucalypt Sawdust for Ethanol Production within a Biorefinery Approach

Engineered yeasts and lignocellulosic biomaterials: shaping a new dimension for biorefinery and global bioeconomy

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