Enhanced production of amylase, pyruvate and phenolic compounds from glucose by light‐driven Aspergillusniger–CuS nanobiohybrids

Abstract

BACKGROUND:The demand for value-added compounds such as amylase, pyruvate and phenolic compounds produced by biological methods has prompted the rapid development of advanced technologies for their enhanced production. Nanobiohybrids (NBs) make use of both the microbial properties of whole-cell microorganisms and the light-harvesting efficiency of semiconductors. Photosynthetic NBs were constructed that link the biosynthetic pathways of Aspergillus niger with CuS nanoparticles. RESULTS: In this work, NB formation was confirmed by negative values of the interaction energy, i.e., 2.31 × 10 8 to −5.52 × 10 8 kJ mol −1 for CuS-Che NBs, whereas for CuS-Bio NBs the values were −2.31 × 10 8 to −4.62 × 10 8 kJ mol −1 for CuS-Bio NBs with spherical nanoparticle interaction. For CuS-Bio NBs with nanorod interaction, it ranged from −2.3 × 10 7 to −3.47 × 10 7 kJ mol −1 . Further, the morphological changes observed by scanning electron microscopy showed the presence of the elements Cu and S in the energy-dispersive X-ray spectra and the presence of CuS bonds in Fourier transform infrared spectroscopy indicate NB formation. In addition, the quenching effect in photoluminescence studies confirmed NB formation. Production yields of amylase, phenolic compounds and pyruvate amounted to 11.2 ∼mol L −1 , 52.5 ∼mol L −1 and 28 nmol ∼L −1 , respectively, in A. niger-CuS Bio NBs on the third day of incubation in the bioreactor. Moreover, A niger cells-CuS Bio NBs had amino acids and lipid yields of 6.2 mg mL −1 and 26.5 mg L −1 , respectively. Furthermore, probable mechanisms for the enhanced production of amylase, pyruvate and phenolic compounds are proposed.CONCLUSION: Aspergillus niger-CuS NBs were used for the production of the amylase enzyme and value-added compounds such as pyruvate and phenolic compounds. Aspergillus niger-CuS Bio NBs showed a greater efficiency compared to A. niger-CuS Che NBs as the biologically produced CuS nanoparticles had a higher compatibility with A. niger cells.

Biological detoxification and metabolism of inhibitors in corncob acid hydrolysate using Aspergillus niger

“…niger is a commonly found strain of fungi belonging to the lamentous fungi genus Aspergillus and is widely used in the industrial enzyme preparation eld due to its high yield, wide application range, and good safety. The enzyme preparations produced with A. niger include more than 30 different types of enzymes, such as amylase [9] , starch enzyme [10] , tannase [11] , cellulase [12] , hemicellulose [13] , and pectinase [14] . In addition, A. niger has received increasing attention in the liquefaction, alcohol, and glucose manufacturing industries.…”

Biological Detoxification of the Inhibitors in Corncob Acid Hydrolysate Using Aspergillus niger

“…niger is a commonly found strain of fungi belonging to the filamentous fungi genus Aspergillus, and it is widely used in the industrial enzyme preparation field due to its high yield, wide application range, and good safety. The enzyme preparations produced with A. niger include more than 30 different types of enzymes, such as amylase [13,14], starch enzyme [15], tannase [16][17][18], cellulase [19,20], hemicellulose [21,22], and pectinase [23]. In addition, A. niger has played a significant role in industrial fermentation and has received increasing attention in the liquefaction, alcohol, and glucose manufacturing industries [24].…”

In Focus: Application of resilient environmental technologies for waste utilization and resource recovery

“…The following three articles explore three different concepts which are quite valuable. The first article by Priyanka and Lens 4 utilised light‐driven Aspergillus niger –CuS nanobiohybrids to enhance the production of amylase enzyme and value‐added products such as pyruvate and phenolic compounds from glucose. Such innovative studies will provide direction for future research in this area.…”