Enhanced production of acetoin and butanediol in recombinant Enterobacter aerogenes carrying Vitreoscilla hemoglobin gene

Geçkil, Hikmet; Barak, Ze ' ev; Chipman, David M.; Erenler, Sebnem O.; Webster, D. R.; Stark, Benjamin C.

doi:10.1007/s00449-004-0373-1

Cited by 44 publications

(18 citation statements)

References 40 publications

(62 reference statements)

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“…The compound 2,3-butanedione was the most important precursors of alkylpyrazine (28,29). The compound 3-hydroxy-2-butanone, which is the immediate precursor to 2,3-butanediol, can be oxidized to 2,3-butanedione for use as a natural flavour in dairy products (30,31). Furfural and benzaldehyde were also detected in most of the Zhenjiang aromatic vinegars, which were responsible for empyreumatic, caramel and a pleasant almond-like odour.…”

Section: Volatile Compounds In Zhenjiang Aromatic Vinegarmentioning

confidence: 99%

HS-SPME/GC-MS and chemometrics for volatile composition of Chinese traditional aromatic vinegar in the Zhenjiang region

Lü

et al. 2012

J. Inst. Brew.

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The main purpose of this study was to determine the volatile composition of Zhenjiang aromatic vinegar, one of the four famous China-style cereal vinegars, by using headspace solid-phase microextraction (HS-SPME)/gas chromatography-mass spectrum (GC-MS) and chemometrics. For this purpose, the HS-SPME sampling method for the volatile compounds of Zhenjiang aromatic vinegar was optimized by a second-order rotatable central composite experimental design (CCD). A HS extraction of the volatile compounds by incubation on a 65 mm thickness polydimethylsiloxane/divinylbenzene (PDMS/DVB) fibre during 44.2 min at 69.5 C with 1.9 g NaCl add gave the most effective and accurate extraction. By the optimized method, a total of 58 volatile compounds, including 9 alcohols, 13 acids, 16 esters, 5 aldehydes, 4 ketones and 8 heterocycle compounds, were identified from 13 aromatic vinegar samples manufactured in Zhenjiang region. By principal components analysis (PCA), the thirteen vinegar samples were classified into 3 groups, and 10 volatile compounds were chosen as characteristic compounds of Zhenjiang aromatic vinegars.

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Section: Volatile Compounds In Zhenjiang Aromatic Vinegarmentioning

confidence: 99%

HS-SPME/GC-MS and chemometrics for volatile composition of Chinese traditional aromatic vinegar in the Zhenjiang region

Lü

et al. 2012

J. Inst. Brew.

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“…Converti and Perego (2002) conducted metabolic flux analyses with various initial glucose concentration conditions. Geckil et al (2004) tried to increase 2,3-butanediol production by introducing the hemoglobin gene (vgb) of Vitreoscilla.…”

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confidence: 99%

Deletion of lactate dehydrogenase in Enterobacter aerogenes to enhance 2,3-butanediol production

Jung

Song

et al. 2012

Appl Microbiol Biotechnol

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2,3-Butanediol is an important bio-based chemical product, because it can be converted into several C4 industrial chemicals. In this study, a lactate dehydrogenase-deleted mutant was constructed to improve 2,3-butanediol productivity in Enterobacter aerogenes. To delete the gene encoding lactate dehydrogenase, λ Red recombination method was successfully adapted for E. aerogenes. The resulting strain produced a very small amount of lactate and 16.7% more 2,3-butanediol than that of the wild-type strain in batch fermentation. The mutant and its parental strain were then cultured with six different carbon sources, and the mutant showed higher carbon source consumption and microbial growth rates in all media. The 2,3-butanediol titer reached 69.5 g/l in 54 h during fed-batch fermentation with the mutant,which was 27.4% higher than that with the parental strain.With further optimization of the medium and aeration conditions,118.05 g/l 2,3-butanediol was produced in 54 h during fed-batch fermentation with the mutant. This is by far the highest titer of 2,3-butanediol with E. aerogenes achieved by metabolic pathway engineering.

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“…Metabolic changes in other bacteria engineered to express VHb have also been reported [Geckil et al, 2004;Liu et al, 1995;Wei et al, 1998]. The most systematic survey of metabolic effects related to VHb has been done in E. coli using microarrays to assess changes in gene expression at the RNA level [Roos et al, 2004].…”

Section: Introductionmentioning

confidence: 99%

Role of Hemoglobin in Improving Biodegradation of Aromatic Contaminants under Hypoxic Conditions

2008

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Genetic engineering of bacteria using the Vitreoscilla (bacterial) hemoglobin gene has been used to enhance bioremediation of several compounds which are models for, or are themselves, toxic chemicals which may contaminate soil and water. Initial experiments, done mostly in shake flasks, with Escherichia coli, Burkholderia sp. DNT and Pseudomonas aeruginosa demonstrated that expression of Vitreoscilla hemoglobin in heterologous hosts can enhance biodegradation of several aromatic compounds as well as an organophosphorus compound. These studies concentrated for the most part on enhancement of endogenous catabolic capabilities of the hosts; the presence of vgb/VHb enhanced both growth and biodegradation. The initial studies were followed by experiments in systems which more closely approximated conditions that would exist in field applications. These included soil columns, continuous flow reactors and membrane bioreactors. The latter work also enabled calculation of the effects of the presence of vgb/VHb on kinetic parameters such as growth rate, substrate and oxygen utilization rate, and degradation rate of pollutants, etc. Although not always the case, for the most part, and particularly in bioreactors, the advantages due to vgb/VHb were greater under conditions of limited aeration or hypoxic conditions.

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Enhanced production of acetoin and butanediol in recombinant Enterobacter aerogenes carrying Vitreoscilla hemoglobin gene

Cited by 44 publications

References 40 publications

HS-SPME/GC-MS and chemometrics for volatile composition of Chinese traditional aromatic vinegar in the Zhenjiang region

HS-SPME/GC-MS and chemometrics for volatile composition of Chinese traditional aromatic vinegar in the Zhenjiang region

Deletion of lactate dehydrogenase in Enterobacter aerogenes to enhance 2,3-butanediol production

Role of Hemoglobin in Improving Biodegradation of Aromatic Contaminants under Hypoxic Conditions

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