Enhanced Production and Quantitative Evaluation of Nigericin from the Algerian Soil-Living Streptomyces youssoufiensis SF10 Strain

Leulmi, Nassima; Sighel, Denise; Defant, Andrea; Khenaka, Karima; Boulahrouf, Abderrahmane; Mancini, Ines

doi:10.3390/fermentation5010013

Cited by 11 publications

(8 citation statements)

References 33 publications

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“…The higher yield of crude antimicrobial extract obtained using the solid-state fermentation method was consistent with previous reports 59 – 63 . Surprisingly, one study showed that solid-state fermentation conditions are ideal for producing antimicrobial compounds by Streptomyces youssoufiensis SF10 strain 64 .…”

Section: Discussionmentioning

confidence: 99%

Isolation, characterization, anti-MRSA evaluation, and in-silico multi-target anti-microbial validations of actinomycin X2 and actinomycin D produced by novel Streptomyces smyrnaeus UKAQ_23

Qureshi

Bholay

Pankaj

et al. 2021

Sci Rep

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Streptomyces smyrnaeus UKAQ_23, isolated from the mangrove-sediment, collected from Jubail,Saudi Arabia, exhibited substantial antimicrobial activity against methicillin-resistant Staphylococcus aureus (MRSA), including non-MRSA Gram-positive test bacteria. The novel isolate, under laboratory-scale conditions, produced the highest yield (561.3 ± 0.3 mg/kg fermented agar) of antimicrobial compounds in modified ISP-4 agar at pH 6.5, temperature 35 °C, inoculum 5% v/w, agar 1.5% w/v, and an incubation period of 7 days. The two major compounds, K1 and K2, were isolated from fermented medium and identified as Actinomycin X2 and Actinomycin D, respectively, based on their structural analysis. The antimicrobial screening showed that Actinomycin X2 had the highest antimicrobial activity compared to Actinomycin D, and the actinomycins-mixture (X2:D, 1:1, w/w) against MRSA and non-MRSA Gram-positive test bacteria, at 5 µg/disc concentrations. The MIC of Actinomycin X2 ranged from 1.56–12.5 µg/ml for non-MRSA and 3.125–12.5 µg/ml for MRSA test bacteria. An in-silico molecular docking demonstrated isoleucyl tRNA synthetase as the most-favored antimicrobial protein target for both actinomycins, X2 and D, while the penicillin-binding protein-1a, was the least-favorable target-protein. In conclusion, Streptomyces smyrnaeus UKAQ_23 emerged as a promising source of Actinomycin X2 with the potential to be scaled up for industrial production, which could benefit the pharmaceutical industry.

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Section: Discussionmentioning

confidence: 99%

Isolation, characterization, anti-MRSA evaluation, and in-silico multi-target anti-microbial validations of actinomycin X2 and actinomycin D produced by novel Streptomyces smyrnaeus UKAQ_23

Qureshi

Bholay

Pankaj

et al. 2021

Sci Rep

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“…By the co-culture with Streptomyces sp., the formation of phenylacetic acid was observed, a metabolite previously reported from S. humidus cultures showing inhibition on some plant-pathogenic fungi. Otherwise, co-culturing SF10 strain with S. coeruleorubidus neither nigericin nor phenylacetic acid were observed [106].…”

Section: Antimicrobialsmentioning

confidence: 91%

Actinobacteria Derived from Algerian Ecosystems as a Prominent Source of Antimicrobial Molecules

et al. 2019

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Actinobacteria, in particular “rare actinobacteria” isolated from extreme ecosystems, remain the most inexhaustible source of novel antimicrobials, offering a chance to discover new bioactive metabolites. This is the first overview on actinobacteria isolated in Algeria since 2002 to date with the aim to present their potential in producing bioactive secondary metabolites. Twenty-nine new species and one novel genus have been isolated, mainly from the Saharan soil and palm groves, where 37.93% of the most abundant genera belong to Saccharothrix and Actinopolyspora. Several of these strains were found to produce antibiotics and antifungal metabolites, including 17 new molecules among the 50 structures reported, and some of these antibacterial metabolites have shown interesting antitumor activities. A series of approaches used to enhance the production of bioactive compounds is also presented as the manipulation of culture media by both classical methods and modeling designs through statistical strategies and the associations with diverse organisms and strains. Focusing on the Algerian natural sources of antimicrobial metabolites, this work is a representative example of the potential of a closely combined study on biology and chemistry of natural products.

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“…Glycerol inhibited cephamycin c and induced the formation of clavulanic acid by this strain (Saudagar and Singhal, 2007). Leulmi et al (2019) reported that, while D-glucose enhanced nigericin production by Streptomyces youssoufiensis SF10 strain, ribose or mannitol decreased nigericin production, but sucrose or starch inhibited nigericin production. In the present study, both glycerol and glucose stimulated the production of the antimicrobial agent(s), but maltose and starch suppressed the synthesis of the antimicrobial agent(s) by NMF76 strain when supplemented separately to the culture medium.…”

Section: Discussionmentioning

confidence: 93%

Isolation and characterization of Streptomyces sp. NMF76 with potential antimicrobial activity from mangrove sediment, Red Sea, Egypt.

Fahmy¹

2020

Egypt. J. of Aquatic Biolo. and Fish.

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The continuous demand to develop novel antibiotics is increased to replace the currently ineffective antibiotics and combat infectious diseases-which represent one of the main causes of death worldwide-caused by antibiotic resistant pathogens (de Lima Procópio et al. 2012). Actinomycetes, the high GC Gram-positive bacteria, are the most valuable microbial group and have produced about half of the microbial bioactive secondary metabolites. The bioactive compounds of actinomycetes exhibit a wide range of biological activities including antibacterial, antifungal, anticancer, antioxidant, antiparasitic, enzyme inhibitors, and immunosuppressive (Miao and Davies, 2010; Azman et al. 2015). Moreover, actinomycetes degrade the complex substances present in the natural environment and produce many enzymes of industrial interest: protease, lipase, cellulase, amylase, esterase, and L-asparaginase (Usha et al. 2011; He et al. 2012; Yeager et al. 2017). Among actinomycetes, the genus Streptomyces is the primary producer of bioactive compounds, specially antibiotics (Azman et al. 2015).

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Enhanced Production and Quantitative Evaluation of Nigericin from the Algerian Soil-Living Streptomyces youssoufiensis SF10 Strain

Cited by 11 publications

References 33 publications

Isolation, characterization, anti-MRSA evaluation, and in-silico multi-target anti-microbial validations of actinomycin X2 and actinomycin D produced by novel Streptomyces smyrnaeus UKAQ_23

Isolation, characterization, anti-MRSA evaluation, and in-silico multi-target anti-microbial validations of actinomycin X2 and actinomycin D produced by novel Streptomyces smyrnaeus UKAQ_23

Actinobacteria Derived from Algerian Ecosystems as a Prominent Source of Antimicrobial Molecules

Isolation and characterization of Streptomyces sp. NMF76 with potential antimicrobial activity from mangrove sediment, Red Sea, Egypt.

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