Enhanced pretreatment CD25 expression on peripheral blood CD4+ T cell predicts shortened survival in acute myeloid leukemia patients receiving induction chemotherapy

Abstract

Our data indicate that quantification of CD25 expression on peripheral blood CD4+ T cells could become a novel, easily accessible method of shortened survival prognostication of AML patients subjected to standard cytotoxic therapy.

Quantitative Flow Cytometry: Concerns and Recommendations in Clinic and Research

“…These beads are particles labeled with a mixture of fluorochromes at different fluorescent intensity levels (23). In addition, rainbow beads allow setting a fixed output value (target MFI) for each fluorescent channel (3,33,34). In addition, rainbow beads allow setting a fixed output value (target MFI) for each fluorescent channel (3,33,34).…”

“…By this principle, designated MESF beads can be used to create a standard curve of MFI versus MESF values can translate MFI to more universal MESF units. Furthermore, the two methodologies can be combined to receive the best inter-assay precision; first setting target MFI with the aid of the rainbow beads followed by conversion of the MFI values of the sample to universal MESF values (34). It should be noted that it is recommended to further calibrate the assay with cells or particles labeled with a known number of fluorochromes (1,2,5,36).…”

“…The beads can be used for a variety of FC calibrations; starting with quality control for FC performance and laser stability, as previously recommended by EuroFlow and ICSH/ICCS (1,3,29,30). In addition, rainbow beads allow setting a fixed output value (target MFI) for each fluorescent channel (3,33,34). The target MFI is set prior to each experiment, ensuring that the reading is done in the correct and standardized settings that are more accurate than using the same PMT settings through different experiments.…”

“…Figure 4 shows that the results from different flow cytometers with MESF values acquired with MESF beads (Bang laboratories), had similar outcome as opposed to settings set based on negative population expression. Furthermore, the two methodologies can be combined to receive the best inter-assay precision; first setting target MFI with the aid of the rainbow beads followed by conversion of the MFI values of the sample to universal MESF values (34).…”

The Triple Immune Argument; Surveillance/Evasion/ Senescence and the Increased Incidence of Acute Myeloid Leukemia Observed with Age

“…One of these studies demonstrated a significant higher percentage of Tregs than the control and the antileukemic activity of T cells was negatively correlated with Tregs (Schick et al, 2013). Furthermore, another study established a link between the presence of elevated CD25 levels on T helper cells and reduced survival rate (Bołkun et al, 2016). These studies pay the attention towards the Tregs as a factor implicated in impairment of T cell function and antitumor response either directly or indirectly (Wilke et al, 2011).…”