Enhanced plumbagin accumulation in embryogenic cell suspension cultures of Plumbago rosea L. following elicitation

Silja, P. K.; Gisha, G. P.; Satheeshkumar, K.

doi:10.1007/s11240-014-0547-8

Cited by 46 publications

(20 citation statements)

References 50 publications

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“…Different letters indicate significant differences (P < 0.05) within groups using one way ANOVA. plumbagin production has already reported in hairy root cultures (Gangopadhyay et al, 2011) and cell suspension cultures of P. rosea (Komaraiah et al, 2002;Silja et al, 2014). The addition of elicitors to in vitro cultures are helpful for understanding plant reaction to microbial threat, stress conditions as well as for enhancing production of valuable secondary metabolites.…”

Section: Elicitor Treatment For Plumbagin Productionmentioning

confidence: 99%

Establishment of adventitious root cultures from leaf explants of Plumbago rosea and enhanced plumbagin production through elicitation

Silja

Satheeshkumar

2015

Industrial Crops and Products

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Section: Elicitor Treatment For Plumbagin Productionmentioning

confidence: 99%

Establishment of adventitious root cultures from leaf explants of Plumbago rosea and enhanced plumbagin production through elicitation

Silja

Satheeshkumar

2015

Industrial Crops and Products

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“…In addition, the plumbagin production (20.28 mg/g DW) by P. indica root cultures that were simultaneously treated with HS and in situ adsorption using Diaion ® HP‐20 was much higher than those previously reported in embryogenic cell suspension cultures (1.13 mg/g DW), and root cultures (1.04 mg/g DW) of P. indica , Drosera burmanii in vitro plantlets (8.8 mg/g DW) , and P. indica hairy root cultures in a bioreactor system (13.16 mg/g DW) . This simultaneously treated root culture system was therefore considered to be suitable for development of a practical alternative method for producing plumbagin.…”

Section: Resultsmentioning

confidence: 58%

Simultaneous heat shock and in situ adsorption enhance plumbagin production in Plumbago indica root cultures

Jaisi

Panichayupakaranant

2016

Engineering in Life Sciences

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Plumbago indica L. is an important source of plumbagin, a commercially valuable bioactive compound. However, the uses of plumbagin are limited due to its low supply as well as low yields and slow growth of the plant sources. This study evaluated the use of a simple, easy, and low-cost approach using heat shock (HS) and ultrasound (US), and an in situ adsorption using a nonpolar copolymer adsorbent styrene-divynilbenzene resin (Diaion R HP-20) to enhance plumbagin production in Plumbago indica root cultures. Treatment with HS (60°C) for 10 min significantly increased the production of plumbagin (5.51 mg/g DW) by up to five-fold, compared to the level in untreated root cultures (1.14 mg/g DW). In contrast, treatments with US alone or with HS treatment produced no satisfactory increase of plumbagin production. However, combined treatment of a 20-day-old root culture with HS (60°C, for 10 min) in the presence of Diaion R HP-20 (10 g/L) markedly increased the production up to 20.28 mg/g DW of plumbagin that was almost 14-fold higher, compared to the level in an untreated root culture. Such an increase would be sufficient for commercial applications of this method to produce plumbagin.

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“…This difference is because of the plant nature, date palm is a tree species and needs more days to culture when compared to herbs and shrubs. In the Indian leadwort, Plumbago rosea, embryogenic cell suspension culture the FW and DW also reached their maximum at the stationary phase of growth (Silja;Gisha;Satheeshkumar, 2014). Recently, the similar pattern of growth curve was observed with respect to FW and DW in the common hazel, Corylus avellana, cell suspension cultures (Gallego et al, 2015).…”

Section: Kinetics Of Cell Growthmentioning

confidence: 64%

Cell suspension culture as a means to produce polyphenols from date palm (Phoenix dactylifera L.)

Naik

Al-Khayri

2018

Ciênc. agrotec.

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Date palm accumulates a wide range of secondary metabolites high in nutritional and therapeutic value. In the present study, date palm (Phoenix dactylifera L., cv. Shaishi) shoot-tip-induced callus was used to establish cell suspension cultures in Murashige and Skoog (MS) liquid medium containing 1.5 mg L-1 2-isopentenyladenine (2iP) and 10 mg L-1 naphthaleneacetic acid (NAA). To study the growth kinetics, cultures were maintained for 12 weeks during which weekly measurements were carried out to determine the biomass accumulation based on packed cell volume (%), fresh weight and dry weight (g). In addition, weekly determination of polyphenols (catechin, caffeic acid, kaempferol, and apigenin) was carried out using high performance liquid chromatography (HPLC). The 11-week-old culture was found highest in the production of biomass (62.9 g L-1 fresh weight and 7.6 g L-1 dry weight) and polyphenols (catechin-155.9 µg L-1, caffeic acid-162.7 µg L-1, kaempferol-89.7 µg L-1, and apigenin-242.7 µg L-1) from the cell suspension cultures. This is the first report on the production of polyphenols from the cell suspension culture of date palm. This study facilitates further development of large-scale production of polyphenols and the utilization of bioreactors.

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Enhanced plumbagin accumulation in embryogenic cell suspension cultures of Plumbago rosea L. following elicitation

Cited by 46 publications

References 50 publications

Establishment of adventitious root cultures from leaf explants of Plumbago rosea and enhanced plumbagin production through elicitation

Establishment of adventitious root cultures from leaf explants of Plumbago rosea and enhanced plumbagin production through elicitation

Simultaneous heat shock and in situ adsorption enhance plumbagin production in Plumbago indica root cultures

Cell suspension culture as a means to produce polyphenols from date palm (Phoenix dactylifera L.)

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