Enhanced Platelet Aggregation as a Risk Factor for Progress and Complications of Vascular Disease. New Findings with a Platelet Aggregation Test (PAT III) and on the Dependence of Different Aggregation Tests on Morphologic Platelet Changes

Breddin, K; Krzywanek, H. J.; Ziemen, J.; Hans, Bauer; Grun, H

doi:10.1007/978-3-642-66609-4_7

Cited by 9 publications

(6 citation statements)

References 3 publications

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“…The lipid phase separation observed in I(12,3)-labeled rat heart plasma membranes (between 21 and 32 ~ might similarly affect various ion transport processes mediated by the sarcolemma (Gordon et al, 1978). In the case of platelets, incubation at cold temperatures induces a shape change closely resembling that caused by ADP at 37 ~ (White & Krivit, 1967) ; moreover, the use of low temperatures may induce platelets to either spontaneously aggregate or to become hyperaggregable in response to ADP additions (Breddin et al, 1977;. Although it has been proposed that these temperature effects may be simply due to conformational changes in platelet membrane proteins (Katlove & Alexander, 1971), our results also suggest the possibility that temperature-mediated decreases in the lipid fluidity are involved.…”

Section: The Effects Of Temperature On Platelet Membrane Fluidity Andmentioning

confidence: 95%

Effects of calcium, lanthanum, and temperature on the fluidity of spin-labeled human platelets

et al. 1980

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Previous platelet studies have shown that calcium plays important roles in stimulus-secretion coupling, aggregation, and other membrane-associated functions. In addition, lanthanum induces platelet aggregation and the platelet release reaction and also influences platelet responsiveness to various stimuli. The spin-label results presented here suggest that one mechanism through which calcium and lanthanum mediate their effects on platelet functions may be by decreasing the lipid fluidity of the surface membrane. The structure of platelet membrane lipids was examined with the spin-label method. Washed human platelets were labeled with the 5-, 12- and 16-nitroxide stearic acid spin probes. Order parameters which measure the fluidity of the lipid environment of the incorporated probe may be calculated from the electron spin resonance (ESR) spectra of 5-nitroxide stearate [I(12,3)]-labeled cells. Evidence is presented which indicates that these spectra principally reflect properties of the platelet surface membrane lipids. The membrane fluidity increased with temperature for the range 17 to 37 degrees C. Either calcium or lanthanum additions to intact cells increased the rigidity of the platelet membranes at 37 degrees C, although the La3+ effect was larger and occurred at lower concentrations than that of Ca2+. For example, addition of 1 mM La3+ or 4 mM Ca2+ increased the order parameter of I(12,3)-labeled platelets by 4.3 +/- 1.7% or 2.1 +/- 0.5%. Preliminary studies conducted on purified platelet plasma membranes labeled with I(12,3) indicated that 1 mM LaCl3 or 4 mM CaCl2 additions similarly decreased the lipid fluidity at 37 degrees C. The above cation-induced effects on the fluidity of whole platelets were reversed by the use of the divalent cation-chelating agent ethylene glycol-bis-(beta-aminoethyl ether)-N,N'-tetra-acetic acid (EGTA). Lastly, lanthanum (0.2-1 mM) caused rapid aggregation of platelets which were suspended in a 50-mM Tris buffer pH 7.4 that did not contain adenosine.

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Section: The Effects Of Temperature On Platelet Membrane Fluidity Andmentioning

confidence: 95%

Effects of calcium, lanthanum, and temperature on the fluidity of spin-labeled human platelets

et al. 1980

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“…Measurements were made of both spontaneous platelet aggregation (aggregation without the addition of aggregation-inducing agents) and induced platelet aggregation (aggregation after the addition of aggregation-inducing agents). Spontaneous platelet aggregation was measured using the photometric platelet aggregation test (PAT 111) of Breddin et al (1977).…”

Section: Measurements Of Platelet Aggregationmentioning

confidence: 99%

Platelet aggregation and multiple sclerosis

Neu¹,

Prosiegel

Pfaffenrath³

2009

Acta Neurologica Scandinavica

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Measurements of blood platelet aggregation were carried out in 30 patients suffering from multiple sclerosis (MS) and in 15 healthy individuals. Compared with the control group, the MS patients showed an increase in both spontaneous and induced (ADP and serotonin) platelet aggregation. The possible pathogenetic significance of these results is discussed.Key words: Increased platelet aggregationfrequency and possible pathogenetic value in multiple sclerosis.

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“…In contrast to other commonly used aggregation tests in which aggregation is induced by the addition of an aggregating agent, Breddin's test studies the spontaneous aggregation of platelets in their normal plasma environment. In patients with thromboembolic disease, diabetes or progressive athero sclerosis, this test shows a high incidence of enhanced platelet aggregation (Breddin andHoff 1968, andBreddin, Krzywanek, et al 1974). It has even been maintained that enhanced platelet aggregation represents an independent risk factor for thromboembolic complications, especially in patients with advanced atherosclerosis (Breddin, Krzywanek, Ziemen et al 1977).…”

Section: Discussionmentioning

confidence: 99%

“…Plastic slides were covered with the diluted plasma for 30 min and then rinsed with citrated saline, fixed in a 40% formaldehyde solution for 5 min, oxidized in a KMnO4N/10 solution again for 5 min and finally Giemsa stained. Platelet aggregation was evaluated at microscope examination by conventional staging in 5 grades, the last two of which (grade IV and V) are considered to be abnormal (Breddin andHoff 1968, andBreddin, Krzywanek et al 1974). Staging of platelet aggregation was done at the end of the trial by three independent skilled investigators who were uninformed as to patient identity and the treatment.…”

Section: Methodsmentioning

confidence: 99%

“…Our aim was to investigate further the antiplatelet effect of sulfinpyrazone and to ascertain whether this drug is also effective in inhibiting an enhanced spontaneous platelet aggregability. To this end, we performed a randomized double-blind crossover trial in patients with enhanced in vitro spontaneous platelet aggregation, which is known to occur in more than half of those subjects with thrombotic complications of vascular disease (Breddin et al 1977).…”

Section: Introductionmentioning

confidence: 99%

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