Enhanced photo-H2 production by Rhodopseudomonas faecalis RLD-53 immobilization on activated carbon fibers

Xie, Guo-Jun; Liu, Bingfeng; Ding, Jie; Xing, Defeng; Ren, Hongyan; Guo, Wei; Ren, Nanqi

doi:10.1016/j.biombioe.2012.05.002

Cited by 38 publications

(15 citation statements)

References 35 publications

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“…The batch culture experiments were carried out in triplicate with 80 ml of the medium in 100 ml sealed reactors and filled with argon to maintain anaerobic conditions [43]. The reactors were autoclaved at 121°C for 15 min.…”

Section: Methodsmentioning

confidence: 99%

Photo-fermentative bacteria aggregation triggered by L-cysteine during hydrogen production

Xie

Liu

Xing

et al. 2013

Biotechnol Biofuels

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BackgroundHydrogen recovered from organic wastes and solar energy by photo-fermentative bacteria (PFB) has been suggested as a promising bioenergy strategy. However, the use of PFB for hydrogen production generally suffers from a serious biomass washout from photobioreactor, due to poor flocculation of PFB. In the continuous operation, PFB cells cannot be efficiently separated from supernatant and rush out with effluent from reactor continuously, which increased the effluent turbidity, meanwhile led to increases in pollutants. Moreover, to replenish the biomass washout, substrate was continuously utilized for cell growth rather than hydrogen production. Consequently, the poor flocculability not only deteriorated the effluent quality, but also decreased the potential yield of hydrogen from substrate. Therefore, enhancing the flocculability of PFB is urgent necessary to further develop photo-fermentative process.ResultsHere, we demonstrated that L-cysteine could improve hydrogen production of Rhodopseudomonas faecalis RLD-53, and more importantly, simultaneously trigger remarkable aggregation of PFB. Experiments showed that L-cysteine greatly promoted the production of extracellular polymeric substances, especially secretion of protein containing more disulfide bonds, and help for enhancement stability of floc of PFB. Through formation of disulfide bonds, L-cysteine not only promoted production of EPS, in particular the secretion of protein, but also stabilized the final confirmation of protein in EPS. In addition, the cell surface elements and functional groups, especially surface charged groups, have also been changed by L-cysteine. Consequently, absolute zeta potential reached a minimum value at 1.0 g/l of L-cysteine, which obviously decreased electrostatic repulsion interaction energy based on DLVO theory. Total interaction energy barrier decreased from 389.77 KT at 0.0 g/l of L-cysteine to 127.21 kT at 1.0 g/l.ConclusionsThus, the strain RLD-53 overcame the total energy barrier and flocculated effectively. After a short settlement, the biomass rush out will be significantly reduced and the effluent quality will be greatly improved in the continuous operation. Furthermore, aggregation of PFB could enable high biomass hold-up of photobioreactor, which allows the photobioreactor to operate at low hydraulic retention time and high organic loading rate. Therefore, the described flocculation behaviour during photo-hydrogen production is potentially suitable for practicable application.

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Section: Methodsmentioning

confidence: 99%

Photo-fermentative bacteria aggregation triggered by L-cysteine during hydrogen production

Xie

Liu

Xing

et al. 2013

Biotechnol Biofuels

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“…The ACFs used in this study were purchased from Sinocarb Carbon Fibers Co., Ltd. (Anshan, China) with specific surface area of 1500 m 2 g −1 . Based on previous test (Xie et al ., ), the length and amount of the modified ACFs were 1 mm and 0.8 g L −1 in this study. Prior to use, ACFs were firstly washed with acetone to remove the surface contamination, and then dried in a vacuum oven until constant weight was obtained.…”

Section: Methodsmentioning

confidence: 97%

“…Several previous works have demonstrated that ACF was a satisfactory immobilization carrier for photo‐fermentative bacteria with good biocompatibility and high immobilization ability (Xie et al ., ,b). Moreover, it was reported that modification can change the surface characteristics and the adsorption properties of ACFs, which had great influence on the improvement of immobilization capacity of ACFs (Oh et al ., ; Liu et al ., ).…”

Section: Introductionmentioning

confidence: 99%

“…This is because bacterial immobilization can maintain high cell density in the photoreactor, keep the activity of bacterial cells, and increase the reaction rate (Lee et al, 2011b). Entrapment and attachment are normally used methods to immobilize photo-fermentative bacteria Xie et al, 2012a). In comparison with entrapment, attachment offers many advantages and is more hopeful, because most carriers have good biological compatibility and can be repeatedly used in the reactors (Tian et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

“…In comparison with entrapment, attachment offers many advantages and is more hopeful, because most carriers have good biological compatibility and can be repeatedly used in the reactors (Tian et al, 2010). Activated carbon fibers (ACFs) were considered as a potential material for bacterial immobilization owing to the large specific surface area, high mechanical characteristic, and extraordinary adsorption capability (Qu et al, 2007;Xie et al, 2012a). In addition, the immobilization carriers would not prevent the bacteria absorbing the light as the fluidization of carriers could dilute and disperse the light over the reactor which is beneficial to the utilization of light (Show et al, 2011).…”

Section: Introductionmentioning

confidence: 99%

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Carrier modification and its application in continuous photo‐hydrogen production using anaerobic fluidized bed photo‐reactor

et al. 2013

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Poor hydrogen production performance and low biomass limit the practical application of photo-fermentation. To improve the immobilization capability of bacteria and hydrogen production performance, activated carbon fibers (ACFs) were modified by acidic, alkaline, and neutral solutions. The modified ACFs were further used in the anaerobic fluidized bed photo-reactor (AFBPR) to explore its continuous operation characteristics. Results showed that among the three reagents, nitric acid was the most efficient for ACF modification, and the maximum yield and production rate of hydrogen increased between about 33.6% and 65.8% compared to the control. Furthermore, with the optimal influent glutamate concentration (10 mmol L À1 ) and light intensity (4000 lux), the AFBPR gave efficient and stable performance with hydrogen yield of 2.26 mol H 2 mol À1 acetate and hydrogen production rate of 25.8 mL L À1 h À1 . The results showed the potential of using the AFBPR with HNO 3 -modified ACF carriers for the large-scale production of bio-hydrogen.

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Nanomaterials for Biohydrogen Production

Sivagurunathan

Kadier

Mudhoo

et al. 2018

Nanomaterials: Biomedical, Environmental, and Engineering Applications

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Enhanced photo-H2 production by Rhodopseudomonas faecalis RLD-53 immobilization on activated carbon fibers

Cited by 38 publications

References 35 publications

Photo-fermentative bacteria aggregation triggered by L-cysteine during hydrogen production

Photo-fermentative bacteria aggregation triggered by L-cysteine during hydrogen production

Carrier modification and its application in continuous photo‐hydrogen production using anaerobic fluidized bed photo‐reactor

Nanomaterials for Biohydrogen Production

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