Enhanced phosphorus nutrition in monocots and dicots over‐expressing a phosphorus‐responsive type I H⁺‐pyrophosphatase

Abstract

SummaryPlants challenged by limited phosphorus undergo dramatic morphological and architectural changes in their root systems in order to increase their absorptive surface area. In this paper, it is shown that phosphorus deficiency results in increased expression of the type I

H+‐pyrophosphatase IbVP1 promotes efficient iron use in sweet potato [Ipomoea batatas(L.) Lam.]

“…Under stressful conditions, up‐regulation of enzyme expression enables plant cells to use PPi as an energy source and maintain membrane integrity and intracellular transport via hydrolysis‐mediated ion transport (Gaxiola et al ., 2016; Greenway and Gibbs, 2003; Stitt, 1998). Increased abiotic stress tolerance and enhanced growth performance in Pi or nitrogen deficiency were observed during H + ‐PPase overexpression in Arabidopsis (Gaxiola et al ., 2001), tomato(Dong et al ., 2011; Park et al ., 2005; Yang et al ., 2007), tobacco (Khoudi et al ., 2012), rice (Zhang et al ., 2011; Zhao et al ., 2006), maize (Li et al ., 2008), lettuce (Paez‐Valencia et al ., 2013) and cotton (Lv et al ., 2009; Pasapula et al ., 2011). Nevertheless, the role of H + ‐PPase‐induced Fe uptake is unknown.…”

“…Enhanced rhizosphere acidification by increased PM‐H + ‐ATPase activity is strongly associated with up‐regulated H + ‐PPase activity, as demonstrated by the IbVP1 overexpressing sweet potato under normal and Fe‐deficient conditions (Figures 3 and 7) consistent with other reports (Paez‐Valencia et al ., 2013; Yang et al ., 2007). Changes in the rhizosphere represent a central mechanism in plant mineral nutrition, contributing to nutrient solubility and proton‐motive force (Li et al ., 2015; Palmgren, 2001; Palmgren, 1998; Santi and Schmidt, 2009; Zhu et al ., 2009).…”

“…Up‐regulation of H + ‐PPase expression and activity has been reported in plants grown under abiotic stress conditions such as chilling, anoxia, mineral deficiency and salt stress (Maeshima, 2000). Up‐regulated H + ‐PPases induce plant tolerance to various stresses, such as drought, high salinity, and N and Pi deprivation (Arif et al ., 2013; Paez‐Valencia et al ., 2013; Yang et al ., 2007). It also affects auxin and sugar transport and accumulation during root and shoot growth (Gaxiola et al ., 2012; Khadilkar et al ., 2016; Li et al ., 2005; Pasapula et al ., 2011; Pizzio et al ., 2015).…”

Expression of Sucrose Transporter cDNAs Specifically in Companion Cells Enhances Phloem Loading and Long-Distance Transport of Sucrose but Leads to an Inhibition of Growth and the Perception of a Phosphate Limitation

“…Potassium levels were kept constant by balancing KH 2 PO 4 supplements with K 2 SO 4 . To test for rhizosphere acidification by transgenic lines relative to wild-type lines, pH indicator dye bromocresol blue was added to one-half-strength MS medium (without Suc or P supplementations) as described (Yang et al, 2007). High-resolution analysis of root elongation on vertically oriented plates was as previously described (Yazdanbakhsh et al, 2011) Quantitative GUS Analysis…”

Ectopic expression of Arabidopsis H+-pyrophosphatase AVP1 enhances drought resistance in bottle gourd (Lagenaria siceraria Standl.)

“…Furthermore, increased root 17 biomass is a consistent phenotype triggered by the overexpression of H + -PPases (Li et al 2005(Li et al , 2008Park 18 et al 2005a;Yang et al 2007;Lv et al 2008Lv et al , 2009…”