Enhanced phenolic acids production in regenerated shoot cultures of Salvia virgata Jacq. after elicitation with Ag + ions, methyl jasmonate and yeast extract

Dowom, Samaneh Attaran; Abrishamchi, Parvaneh; Radjabian, Tayebeh; Salami, Seyed Alireza

doi:10.1016/j.indcrop.2017.03.043

Cited by 48 publications

(14 citation statements)

References 47 publications

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“…26 The previous studies reported similar results as the present study in that elicitation with MeJa significantly enhanced TPC and TFC in hairy root cultures of other plants. 11,27,28 Elicitation also enhanced the accumulation of hydroxycinnamic acids; particularly, MeJa significantly enhanced chicoric acid accumulation in L. indica L. Similar results were reported for Echinacea purpurea 29 and Echinacea angustifolia. 30 Furthermore, secondary metabolites were reportedly enhanced by increasing exposure duration in hairy root cultures of Salvia miltiorrhiza , 31 Salvia virgata Jacq., 27 and Rubia tinctorum .…”

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confidence: 82%

“…11,27,28 Elicitation also enhanced the accumulation of hydroxycinnamic acids; particularly, MeJa significantly enhanced chicoric acid accumulation in L. indica L. Similar results were reported for Echinacea purpurea 29 and Echinacea angustifolia. 30 Furthermore, secondary metabolites were reportedly enhanced by increasing exposure duration in hairy root cultures of Salvia miltiorrhiza , 31 Salvia virgata Jacq., 27 and Rubia tinctorum . 32 In addition, an elicitor-concentration-dependent increase was observed in American ginseng 28 and in Solanum triobatum L., 10 whereas a combination of exposure duration and concentration was determinant in the case of S. virgata Jacq.…”

supporting

confidence: 82%

“…32 In addition, an elicitor-concentration-dependent increase was observed in American ginseng 28 and in Solanum triobatum L., 10 whereas a combination of exposure duration and concentration was determinant in the case of S. virgata Jacq. 27…”

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confidence: 99%

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Enhancement of Phenolic Compounds and Antioxidative Activities by the Combination of Culture Medium and Methyl Jasmonate Elicitation in Hairy Root Cultures of Lactuca indica L.

Park

Park³

et al. 2019

Natural Product Communications

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Lactuca indica L. has been traditionally used as a wild vegetable and as a medicinal plant for centuries. The various compounds present in it and their biological activities have been extensively reported. Hairy-root culture combined with agrobacterium-meditated metabolic engineering is a useful technique to achieve stable production of biologically active plant compounds. Here, we evaluated the enhancement of secondary metabolites in L. indica L. and their bioactivities by testing culture media composition and the use of an elicitor. Hairy roots were induced and cultured in MS or SH liquid media for 2 weeks prior to treatment with various concentrations of MeJa, for different periods. The resulting phenolic contents and physiological activities were analyzed. Higher total phenolic, flavonoid, and hydroxycinnamic acids contents were attained by elicitation with MeJa. Metabolite accumulation, especially in SH media and in the presence of MeJa, was time dependent. Particularly, accumulation of chicoric acid increased markedly with time. Similarly, we observed time dependent positive and negative responses of antioxidant activity in DPPH and ABTS assays, respectively. As in previous studies, the highest correlation was found between total phenolic content and total flavonoid content. Further, 3,5-DCQA showed the highest correlation with total phenolic content, total flavonoid content, and antioxidant activities in hydroxycinnamic acids. Our data effectively identified optimal culture conditions to increase the accumulation of secondary metabolites and antioxidant activity in hairy roots cultures of L. indica L.

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confidence: 82%

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confidence: 82%

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Enhancement of Phenolic Compounds and Antioxidative Activities by the Combination of Culture Medium and Methyl Jasmonate Elicitation in Hairy Root Cultures of Lactuca indica L.

Park

Park³

et al. 2019

Natural Product Communications

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“…BAP was also more effective than thidiazuron in the proliferation of Salvia brachyodon [9], Salvia nemorosa [10], and Salvia fruticosa [11]. Based on these studies, and those performed on Salvia africana-lutea [12] or Salvia virgata [13], MS medium supplemented with auxin and 0.5 mg/L BAP was used for the establishment of S. viridis culture, and its initial proliferation [5]. However, this protocol for the development of sage shoots revealed that this combination of growth regulators did not give satisfactory multiplication results (only 1.3 shoot per explant).…”

Section: Introductionmentioning

confidence: 99%

The Stimulatory Effect of Purine-Type Cytokinins on Proliferation and Polyphenolic Compound Accumulation in Shoot Culture of Salvia viridis

et al. 2020

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The present study demonstrates hormonal control of Salvia viridis growth and development using four different purine-type cytokinins at different concentrations. The addition of cytokinins significantly increased biomass of cultures, proliferation rate, and, interestingly, secondary metabolite production. The best response in terms of multiplication ratio was recorded on Murashige and Skoog medium supplemented with 0.5 mg/L BPA (N-benzylotetrahydropyranyl adenine), while the greatest biomass accumulation was achieved when supplemented with 1 mg/L m-T (meta-topoline). Quantitative UPLC-DAD analysis of the hydromethanolic extract from S. viridis culture revealed the presence of 12 polyphenols: seven phenolic acids and five phenylethanoids. The highest total content of polyphenolic compounds was found in shoots cultivated on medium with 2 mg/L BPA (18.66 mg/g DW): almost twice that of control shoots. The medium was also the most optimal for the biosynthesis of rosmarinic acid, the predominant phenolic acid. However, the greater phenylethanoid accumulation was stimulated by 1 mg/L m-T: the metabolite content was above three times higher than that found in shoots grown on the control medium (8.03 mg/g DW vs. 2.37 mg/g DW). Hence, it was demonstrated that phytohormones are capable of influencing not only vital physiological processes, but therapeutic potential of plants as well. Therefore, the cytokinin-based sage cultures may be also considered as the alternative sources of bioactive compounds.

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“…Numerous studies have identified 100 μmol L −1 MeJA as an optimum concentration to enhance the biosynthesis of bioactive compounds such as psoralen, valtrate and phenols. [52][53][54] The positive effects of MeJA on the increase in secondary metabolites production have been confirmed in many studiesfor example psoralen production in suspension cultures of Psoralea corylifolia L., 52 production of phenolic acids in in vitro regenerated shoots of Salvia virgata Jacq., 55 gymnemic acid production in suspension cultures of Gymnema sylvestre L., 56 production of phenolic acids in HR cultures of Salvia miltiorrhiza, 22 phenolic metabolism in HR cultures of Daucus carota L., 57 and rosmarinic acid accumulation in HR cultures of Coleus blumei. 58 As the antioxidant capacity of plants is closely associated with their secondary metabolites, 59 MeJA could strongly improve the DPPH and FRAP antioxidant capacity of the elicited HRs depending on the concentration and exposure time.…”

Section: A1 A2 B M C + H 1 H 2 H 3 H 4 C -C º M C + H 1 H 2 C º C -M Hmentioning

confidence: 99%

Agrobacterium rhizogenes mediated transformation of Ficus carica L. for the efficient production of secondary metabolites

et al. 2020

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BACKGROUND Ficus carica L., an ancient source of food and medicines, is rich in valuable nutritional and secondary compounds with antioxidant, antimicrobial, and anticancer effects. The present study is the first attempt to examine hairy root (HR) induction of F. carica (Sabz and Siah) by inoculating the 3‐week‐old shoots and leaves with different strains of Agrobacterium rhizogenes and also to investigate methyl jasmonate (MeJA) elicitation of HRs to produce a fast and high‐yield production method for secondary metabolites. RESULTS The maximum transformation rate (100%) was achieved by inoculating the shoots with Agrobacterium rhizogenes strain A7. Siah HRs elicited with 100 and 200 μmol L−1 MeJA and Sabz HRs with 100 μmol L−1 MeJA showed the highest total phenolic content. The highest flavonoid content was 3.935 mg QE g−1 DW in Siah HRs treated with 200 μmol L−1 MeJA and 2.762 mg QE g−1 DW in Sabz HRs treated with 300 μmol L−1 MeJA. The 2, 2‐diphenyl‐1‐picrylhydrazyl (DPPH) radical scavenging capacity and ferric reducing antioxidant power (FRAP) value of HRs were affected by MeJA treatments. Methyl jasmonate elicitation also significantly enhanced the content of six phenolic acids (gallic acid, caffeic acid, chlorogenic acid, coumaric acid, rosmarinic acid, and cinnamic acid) and three flavonoids (rutin, quercetin, and apigenin). Thymol, a monoterpene phenol, was the main HR compound detected in gas chromatography mass spectrometry (GC–MS) analysis of the essential oils. CONCLUSION Induction of HRs and elicitation of F. carica HRs by MeJA resulted in a significant increase in the production of important phenolic compounds and a significant increase in antioxidant capacity. © 2020 Society of Chemical Industry

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Enhanced phenolic acids production in regenerated shoot cultures of Salvia virgata Jacq. after elicitation with Ag + ions, methyl jasmonate and yeast extract

Cited by 48 publications

References 47 publications

Enhancement of Phenolic Compounds and Antioxidative Activities by the Combination of Culture Medium and Methyl Jasmonate Elicitation in Hairy Root Cultures of Lactuca indica L.

Enhancement of Phenolic Compounds and Antioxidative Activities by the Combination of Culture Medium and Methyl Jasmonate Elicitation in Hairy Root Cultures of Lactuca indica L.

The Stimulatory Effect of Purine-Type Cytokinins on Proliferation and Polyphenolic Compound Accumulation in Shoot Culture of Salvia viridis

Agrobacterium rhizogenes mediated transformation of Ficus carica L. for the efficient production of secondary metabolites

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