Enhanced performance for the analysis of prostaglandins and thromboxanes by liquid chromatography-tandem mass spectrometry using a new atmospheric pressure ionization source

Lubin, Arnaud; Geerinckx, Suzy; Bajic, Steve; Cabooter, Deirdre; Augustijns, Patrick; Cuyckens, Filip; Vreeken, Rob J.

doi:10.1016/j.chroma.2016.02.055

Cited by 25 publications

(32 citation statements)

References 24 publications

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“…The significant difference between ESI and US may result from the fact that the r 2 values are very close to each other. Similar linearity with r 2 values ranging from 0.994 to 0.999 but with no significant difference between US and ESI was previously reported for pharmaceutical compounds [14].…”

Section: Resultssupporting

confidence: 85%

“…Comparatively with ESI, US was proven more performant in analysis of various compounds. The US interface showed a fivefold increase in method sensitivity, with an improved signal intensity, linearity, and repeatability on various matrices in comparison with ESI, for the analysis of prostaglandins and thromboxanes [14]. Similarly, for 24 pharmaceutical and biological compounds, US above ESI improved the dynamic range of analytes at lower concentrations and the sensitivity of late eluting compounds [12].…”

Section: Introductionmentioning

confidence: 99%

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Comparison of electrospray and UniSpray, a novel atmospheric pressure ionization interface, for LC-MS/MS analysis of 81 pesticide residues in food and water matrices

et al. 2019

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In mass spectrometry, the type and design of ionization source play a key role on the performance of a given instrument. Therefore, it is of paramount importance to evaluate newly developed sources for their suitability to analyze food contaminants like pesticide residues. Here, we carried out a head-to-head comparison of key extraction and analytical performance parameters of an electrospray ionization (ESI) source with a new atmospheric pressure ionization source, UniSpray (US). The two interfaces were evaluated in three matrices of different properties (coffee, apple, and water) to determine if multiresidue analysis of 81 pesticides by QuEChERS extraction and LC-MS/MS analysis could be improved. Depending on the matrix and irrespective of the chemical class, US provided a tremendous gain in signal intensity (22- to 32-fold in peak area, 6- to 7-fold in peak height), a threefold to fourfold increase in signal-to-noise ratio, a mild gain in the range of compounds that can be quantified, and up to twofold improvement of recovery. UniSpray offered comparable linearity and precision of the analyses with ESI, and did not affect the ion ratio. A gain in sensitivity of many compounds was observed with US, but in general, the two ionization interfaces did not show significant difference in LOD and LOQ. UniSpray suffered less signal suppression; the matrix effect was in average 3 to 4 times more pronounced, but showed better values than ESI. With no effect on recovery efficiency, US improved the overall process efficiency 3 to 4 times more than ESI. Graphical abstract Electronic supplementary material The online version of this article (10.1007/s00216-019-01886-z) contains supplementary material, which is available to authorized users.

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Section: Resultssupporting

confidence: 85%

Section: Introductionmentioning

confidence: 99%

Comparison of electrospray and UniSpray, a novel atmospheric pressure ionization interface, for LC-MS/MS analysis of 81 pesticide residues in food and water matrices

et al. 2019

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“…Besides sensitivity issues, source design modifications can also result in more robust, repeatable and therefore more reliable data. Very recently, Lubin et al [44] compared a Unispray ionization source, wherein a highly charged metal rod is added between the grounded/neutral ESI nebulizer and the ion inlet of the mass spectrometer (figure 2) with a conventional ESI source for the analysis of prostaglandins and thromboxanes. Analysing diverse sample matrices, an equivalent or better linearity and repeatability could be demonstrated for the Unispray combined with an increase in sensitivity up to a factor 5.…”

Section: (C) Ion Formation and Guidancementioning

confidence: 99%

“…Considering the use of a charged rod positioned at a small distance from the nebulizer to ionize the analytes, this source should be capable of ionizing more volatile compounds compared with a Z-spray ESI source from Waters. However, the ionization principle still has to be characterized [44].…”

Section: (C) Ion Formation and Guidancementioning

confidence: 99%

Quantitative mass spectrometry methods for pharmaceutical analysis

Loos¹,

Schepdael²,

Cabooter³

2016

Phil. Trans. R. Soc. A.

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Quantitative pharmaceutical analysis is nowadays frequently executed using mass spectrometry. Electrospray ionization coupled to a (hybrid) triple quadrupole mass spectrometer is generally used in combination with solid-phase extraction and liquid chromatography. Furthermore, isotopically labelled standards are often used to correct for ion suppression. The challenges in producing sensitive but reliable quantitative data depend on the instrumentation, sample preparation and hyphenated techniques. In this contribution, different approaches to enhance the ionization efficiencies using modified source geometries and improved ion guidance are provided. Furthermore, possibilities to minimize, assess and correct for matrix interferences caused by co-eluting substances are described. With the focus on pharmaceuticals in the environment and bioanalysis, different separation techniques, trends in liquid chromatography and sample preparation methods to minimize matrix effects and increase sensitivity are discussed. Although highly sensitive methods are generally aimed for to provide automated multi-residue analysis, (less sensitive) miniaturized set-ups have a great potential due to their ability for in-field usage. This article is part of the themed issue ‘Quantitative mass spectrometry’.

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“…For example, liquid-liquid extraction for the determine eicosanoids in plasma with LC/MS/ MS, 12 plasma analysis using online-SPE-LC-MS/MS, 13 by using a new atmospheric pressure ionization source to enhance the performance for eicosanoid analysis 14 and human plasma analysis using ultra-fast liquid chromatography (UFLC) for prostaglandins, 15 endocannabinoids and steroids metabolites, and eicosanoids analysis using ultra performance liquid chromatography (UPLC) for plasma sample 16 and urine sample 17 were reported. For that purpose, LC/MS/MS has been used as a suitable technology that enables highly sensitive and simultaneous measurements; however, since the amount of eicosanoids contained in biological samples is extremely small, the analytical quality is significantly influenced by the pre-analytical sample preparation.…”

Section: Introductionmentioning

confidence: 99%

A Simple and Highly Sensitive Quantitation of Eicosanoids in Biological Samples Using Nano-flow Liquid Chromatography/ Mass Spectrometry

Ando

Satomi

2018

ANAL. SCI.

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A simple sample preparation method for eicosanoid was developed by the combination of deproteinization and nanoLC-ESI-MS/MS. Eicosanoids are a group of bioactive lipid mediators, present in trace amounts in the body. Therefore, an analytical method for eicosanoids requires superior sensitivity. The method described in this report, which takes advantage of the highly sensitive power of nanoLC-ESI-MS/MS, enabled a simplification of the sample-preparation process. Eicosanoid extraction was performed just by homogenization in methanol with subsequent phospholipid removal, and then the liquid phase was directly subjected to nanoLC-ESI-MS/MS analysis without a condensation process. The quantitation range achieved 0.01 -100 ng/mL for thromboxane B2, and 0.05 -100 ng/mL for prostaglandin E2, prostaglandin D2, prostaglandin F2, leukotriene B4, 6-keto prostaglandin F1α and 11-dehydro thromboxane B2. Rat brain sample analyses demonstrated the feasibility of the quantification of those seven eicosanoids from biological samples.

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Enhanced performance for the analysis of prostaglandins and thromboxanes by liquid chromatography-tandem mass spectrometry using a new atmospheric pressure ionization source

Cited by 25 publications

References 24 publications

Comparison of electrospray and UniSpray, a novel atmospheric pressure ionization interface, for LC-MS/MS analysis of 81 pesticide residues in food and water matrices

Comparison of electrospray and UniSpray, a novel atmospheric pressure ionization interface, for LC-MS/MS analysis of 81 pesticide residues in food and water matrices

Quantitative mass spectrometry methods for pharmaceutical analysis

A Simple and Highly Sensitive Quantitation of Eicosanoids in Biological Samples Using Nano-flow Liquid Chromatography/ Mass Spectrometry

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