Enhanced penetrative siRNA delivery by a nanodiamond drug delivery platform against hepatocellular carcinoma 3D models

Xu, Jingru; Gu, Meijia; Hooi, Lissa; Toh, Tan Boon; Thng, Dexter Kai Hao; Lim, Jackwee; Chow, Edward Kai‐Hua

doi:10.1039/d1nr03502a

Cited by 26 publications

(16 citation statements)

References 111 publications

(123 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Negatively charged proteins, present in FBS, have been reported to competitively bind to nanocarriers and enhance release ( Ju and Yeo, 2012 ; Wang et al, 2014 ). Xu et al (2021) , also reported that siRNA release from nanodiamonds was increased by enhancing the FBS percentage from 1.25% to 10% in the release media. In the intracellular environment, the presence of various soluble biological polyanions can competitively replace and dissociate the nucleic acids from nanocomplexes ( Cozzolino et al, 2021 ).…”

Section: Resultsmentioning

confidence: 89%

Trimethyl-Chitosan Coated Gold Nanoparticles Enhance Delivery, Cellular Uptake and Gene Silencing Effect of EGFR-siRNA in Breast Cancer Cells

Baghani

Heris

Khonsari

et al. 2022

Front. Mol. Biosci.

View full text Add to dashboard Cite

Purpose: Despite the promising therapeutic effects of gene silencing with small interfering RNAs (siRNAs), the challenges associated with delivery of siRNAs to the tumor cells in vivo, has greatly limited its clinical application. To overcome these challenges, we employed gold nanoparticles modified with trimethyl chitosan (TMC) as an effective delivery carrier to improve the stability and cellular uptake of siRNAs against epidermal growth factor receptor (EGFR) that is implicated in breast cancer.Methods: AuNPs were prepared by the simple aqueous reduction of chloroauric acid (HAuCl4) with ascorbic acid and coated with synthesized TMC. EGFR-siRNA was then complexed with the AuNPs-TMC via electrostatic interaction to make AuNPs-TMC/EGFR-siRNA with a w/w ratio of 10:1. Nanoparticles were assessed for physicochemical characteristics and in vitro cellular behavior on MCF-7 breast cancer cell line.Results: Spherical and positively charged AuNPs-TMC (67 nm, +45 mV) were successfully complexed with EGFR-siRNA (82 nm, +11 mV) which were able to retard the gene migration completely. Confocal microscopy and flow cytometry analysis demonstrated complete cellular uptake of Cy5 labeled AuNPs-TMC in the MCF-7 cells after 4 h incubation. MTT test after 48 h incubation showed that the AuNPs-TMC were safe but when combined with EGFR-siRNA exert significant cytotoxicity while the cell viability was about 50%. These nanocomplexes also showed a high gene expression knockdown (86%) of EGFR and also a high apoptosis rate (Q2 + Q3 = 18.5%) after 24 h incubation.Conclusion: This study suggests that the simply synthesized AuNPs-TMC are novel, effective, and promising nanocarriers for siRNA delivery, and AuNPs-TMC/EGFR-siRNA appears to be a potential therapeutic agent for breast cancer treatment.

show abstract

Section: Resultsmentioning

confidence: 89%

Trimethyl-Chitosan Coated Gold Nanoparticles Enhance Delivery, Cellular Uptake and Gene Silencing Effect of EGFR-siRNA in Breast Cancer Cells

Baghani

Heris

Khonsari

et al. 2022

Front. Mol. Biosci.

View full text Add to dashboard Cite

show abstract

“…7(a)). 168 Other studies revealed that protamine sulphate-coated ND systems facilitated not only the delivery of microRNA mimics for the treatment of esophageal cancer and triple negative breast cancer, but also the signal enhancement for in vivo tumour imaging when compared to uncoated NDs. 169,170 Our group also reported the delivery of antisense oligonucleotide (ASO) 4625 using NDs coated with TAT and NLS.…”

Section: Gene Therapymentioning

confidence: 99%

Versatile nanodiamond-based tools for therapeutics and bioimaging

et al. 2023

View full text Add to dashboard Cite

show abstract

“…Thus, siRNA exerts its therapeutic effect. 141 Theranostic Applications of Carbon Nanodots. Carbon dots (CD) are carbon analogues and were first discovered in 2004 during the preparation of single-walled carbon nanotubes.…”

Section: Theranostic Applicationsmentioning

confidence: 99%

“…ND-siRNA complexes absorbed by the cell enter the cytoplasm through an endosomal escape mechanism and protect the siRNA against degradation. Thus, siRNA exerts its therapeutic effect …”

Section: Carbon-based Nanoparticles For Theranostic Applicationsmentioning

confidence: 99%

Multifunctional Carbon-Based Nanoparticles: Theranostic Applications in Cancer Therapy and Diagnosis

Hosseini

Mohammadnejad

Najafi-Taher

et al. 2023

ACS Appl. Bio Mater.

View full text Add to dashboard Cite

Cancer diagnosis and treatment are the most critical challenges in modern medicine. Conventional cancer treatments no longer meet the needs of the health field due to the high rate of mutations and epigenetic factors that have caused drug resistance in tumor cells. Hence, the search for unique methods and factors is quickly expanding. The development of nanotechnology in medicine and the search for a system to integrate treatment and diagnosis to achieve an effective approach to overcome the known limitations of conventional treatment methods have led to the emergence of theranostic nanoparticles and nanosystems based on these nanoparticles. An influential group of these nanoparticles is carbon-based theranostic nanoparticles. These nanoparticles have received significant attention due to their unique properties, such as electrical conductivity, high strength, excellent surface chemistry, and wide range of structural diversity (graphene, nanodiamond, carbon quantum dots, fullerenes, carbon nanotubes, and carbon nanohorns). These nanoparticles were widely used in various fields, such as tissue engineering, drug delivery, imaging, and biosensors. In this review, we discuss in detail the recent features and advances in carbon-based theranostic nanoparticles and the advanced and diverse strategies used to treat diseases with these nanoparticles.

show abstract

Enhanced penetrative siRNA delivery by a nanodiamond drug delivery platform against hepatocellular carcinoma 3D models

Abstract: Comprehensive analysis of hepatocellular carcinoma 3D models revealed enhanced penetrative siRNA delivery by a nanodiamonds compared to liposomes. Nanodiamonds were able to improve siRNA's gene knockdown and anti-cancer effects in 3D tumor models.

Cited by 26 publications

References 111 publications

Trimethyl-Chitosan Coated Gold Nanoparticles Enhance Delivery, Cellular Uptake and Gene Silencing Effect of EGFR-siRNA in Breast Cancer Cells

Trimethyl-Chitosan Coated Gold Nanoparticles Enhance Delivery, Cellular Uptake and Gene Silencing Effect of EGFR-siRNA in Breast Cancer Cells

Versatile nanodiamond-based tools for therapeutics and bioimaging

Multifunctional Carbon-Based Nanoparticles: Theranostic Applications in Cancer Therapy and Diagnosis

Contact Info

Product

Resources

About