Enhanced Oxidative Stress Resistance through Activation of a Zinc Deficiency Transcription Factor in <i>Brachypodium distachyon</i>

Glover-Cutter, Kira; Alderman, S. C.; Dombrowski, James E.; Martin, Ruth C.

doi:10.1104/pp.114.240457

Cited by 15 publications

(19 citation statements)

References 101 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Ninety‐six Brachypodium bZIP genes have been identified and three (BdbZIP11, BdbZIP32, and BdbZIP33) were shown to group with Arabidopsis F‐group bZIPs in phylogenetic analysis (Liu & Chu, ). BdbZIP11 is referred to as BdbZIP10 by Glover‐Cutter, Alderman, Dombrowski, and Martin () and this has been implicated in oxidative stress and Zn‐deficiency responses in Brachypodium. HvbZIP62 and HvbZIP56 are most closely related to BdbZIP11 (76% and 65% identity respectively; Table S4).…”

Section: Resultsmentioning

confidence: 99%

“…Targeting from the cytoplasm to the nucleus as part of the mechanism for activation of F‐group bZIPs has been supported for other bZIPs. For example, BdbZIP10/11 showed enhanced levels in the nucleus following exposure to oxidative stress, and this was suggested to be due to post‐translational modifications enhancing stability or directing subcellular localization (Glover‐Cutter et al, ). Yang et al () also found that AtbZIP24 was re‐distributed preferentially to the nucleus in response to salt stress.…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

F‐group bZIPs in barley—a role in Zn deficiency

Nazri

Griffin

Peaston

et al. 2017

Plant Cell & Environment

View full text Add to dashboard Cite

Zinc (Zn) deficiency negatively impacts the development and health of plants and affects crop yield. When experiencing low Zn, plants undergo an adaptive response to maintain Zn homeostasis. We provide further evidence for the role of F‐group transcription factors, AtbZIP19 and AtbZIP23, in responding to Zn deficiency in Arabidopsis and demonstrate the sensitivity and specificity of this response. Despite their economic importance, the role of F‐group bZIPs in cereal crops is largely unknown. Here, we provide new insights by functionally characterizing these in barley (Hordeum vulgare), demonstrating an expanded number of F‐group bZIPs (seven) compared to Arabidopsis. The F‐group barley bZIPs, HvbZIP56 and HvbZIP62, partially rescue the Zn‐dependent growth phenotype and ZIP‐transporter gene regulation of an Arabidopsis bzip19‐4 bzip23‐2 mutant. This supports a conserved mechanism of action in adapting to Zn deficiency. HvbZIP56 localizes to the cytoplasm and nucleus when expressed in Arabidopsis and tobacco. Promoter analysis demonstrates that the barley ZIP transporters that are upregulated under Zn deficiency contain cis Zn‐deficiency response elements (ZDREs). ZDREs are also found in particular barley bZIP promoters. This study represents a significant step forward in understanding the mechanisms controlling Zn responses in cereal crops, and will aid in developing strategies for crop improvement.

show abstract

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

F‐group bZIPs in barley—a role in Zn deficiency

Nazri

Griffin

Peaston

et al. 2017

Plant Cell & Environment

View full text Add to dashboard Cite

show abstract

“…Zinc deficiency was previously shown to induce oxidative stress related genes in wheat [ 16 ]. Furthermore, the BdbZIP10 (Bradi1g30140) TF, when overexpressed in Brachypodium , conferred enhanced oxidative stress resistance and increased viability when plants or cells were exposed to N,N′-dimethyl-4,4′-bipyridinium dichloride (common name: paraquat) [ 17 ], an herbicide which induces oxidative stress in plants [ 18 , 19 ]. This BdbZIP10 TF is the homolog of Arabidopsis bZIP19 and Arabidopsis bZIP23 TFs, which have been characterized for their role in adaptation to zinc deficiency in Arabidopsis .…”

Section: Introductionmentioning

confidence: 99%

Genome-wide (ChIP-seq) identification of target genes regulated by BdbZIP10 during paraquat-induced oxidative stress

2018

Self Cite

View full text Add to dashboard Cite

BackgroundbZIP transcription factors play a significant role in many aspects of plant growth and development and also play critical regulatory roles during plant responses to various stresses. Overexpression of the Brachypodium bZIP10 (Bradi1g30140) transcription factor conferred enhanced oxidative stress tolerance and increased viability when plants or cells were exposed to the herbicide paraquat. To gain a better understanding of genes involved in bZIP10 conferred oxidative stress tolerance, chromatin immunoprecipitation followed by high throughput sequencing (ChIP-Seq) was performed on BdbZIP10 overexpressing plants in the presence of oxidative stress.ResultsWe identified a transcription factor binding motif, TGDCGACA, different from most known bZIP TF motifs but with strong homology to the Arabidopsis zinc deficiency response element. Analysis of the immunoprecipitated sequences revealed an enrichment of gene ontology groups with metal ion transmembrane transporter, transferase, catalytic and binding activities. Functional categories including kinases and phosphotransferases, cation/ion transmembrane transporters, transferases (phosphorus-containing and glycosyl groups), and some nucleoside/nucleotide binding activities were also enriched.ConclusionsBrachypodium bZIP10 is involved in zinc homeostasis, as it relates to oxidative stress.Electronic supplementary materialThe online version of this article (10.1186/s12870-018-1275-8) contains supplementary material, which is available to authorized users.

show abstract

“…Regenerating shoots were removed as they developed and placed on MS sucrose medium in magenta boxes under the same conditions. Plantlets were transferred to soil, acclimated under a plastic dome for a week and then grown as described in an earlier paper [16]. Sunshine Growing Mix (Sun Gro Horticulture Inc., Bellevue, WA) was used for all greenhouse plantings.…”

Section: Brachypodium Transformation Growing Conditions and Identifimentioning

confidence: 99%

“…Wheat lip19 bZIP transcription factor (Wlip19), which was activated by low temperature, drought and ABA, conferred improved abiotic stress tolerance when overexpressed in tobacco [15]. Overexpression of a Brachypodium bZIP10 transcription factor (Bradi1g30140.1) in Brachypodium induced expression of several protective oxidative stress genes, leading to increased oxidative stress tolerance [16], with minimal effects on plant growth and development. A wheat TabZIP60 gene, which was strongly induced by polyethylene glycol, salt, cold and abscisic acid (ABA) treatments, was able to improve drought, salt and freezing stress tolerance when overexpressed in Arabidopsis, with the seedlings displaying increased sensitivity to ABA [17].…”

Section: Introductionmentioning

confidence: 99%

Transcriptome analysis of responses in Brachypodium distachyon overexpressing the BdbZIP26 transcription factor

2020

Self Cite

View full text Add to dashboard Cite

Background: Biotic and abiotic stresses are the major cause of reduced growth, persistence, and yield in agriculture. Over the past decade, RNA-Sequencing and the use of transgenics with altered expression of stress related genes have been utilized to gain a better understanding of the molecular mechanisms leading to salt tolerance in a variety of species. Identification of transcription factors that, when overexpressed in plants, improve multiple stress tolerance may be valuable for crop improvement, but sometimes overexpression leads to deleterious effects during normal plant growth. Results: Brachypodium constitutively expressing the BdbZIP26:GFP gene showed reduced stature compared to wild type plants (WT). RNA-Seq analysis comparing WT and bZIP26 transgenic plants revealed 7772 differentially expressed genes (DEGs). Of these DEGs, 987 of the DEGs were differentially expressed in all three transgenic lines. Many of these DEGs are similar to those often observed in response to abiotic and biotic stress, including signaling proteins such as kinases/phosphatases, calcium/calmodulin related proteins, oxidases/reductases, hormone production and signaling, transcription factors, as well as disease responsive proteins. Interestingly, there were many DEGs associated with protein turnover including ubiquitin-related proteins, F-Box and U-box related proteins, membrane proteins, and ribosomal synthesis proteins. Transgenic and control plants were exposed to salinity stress. Many of the DEGs between the WT and transgenic lines under control conditions were also found to be differentially expressed in WT in response to salinity stress. This suggests that the over-expression of the transcription factor is placing the plant in a state of stress, which may contribute to the plants diminished stature. Conclusion: The constitutive expression of BdbZIP26:GFP had an overall negative effect on plant growth and resulted in stunted plants compared to WT plants under control conditions, and a similar response to WT plants under salt stress conditions. The results of gene expression analysis suggest that the transgenic plants are in a constant state of stress, and that they are trying to allocate resources to survive.

show abstract

Enhanced Oxidative Stress Resistance through Activation of a Zinc Deficiency Transcription Factor in Brachypodium distachyon

Cited by 15 publications

References 101 publications

F‐group bZIPs in barley—a role in Zn deficiency

F‐group bZIPs in barley—a role in Zn deficiency

Genome-wide (ChIP-seq) identification of target genes regulated by BdbZIP10 during paraquat-induced oxidative stress

Transcriptome analysis of responses in Brachypodium distachyon overexpressing the BdbZIP26 transcription factor

Contact Info

Product

Resources

About