Enhanced oxidative stress in GH-transgenic rat and acromegaly in humans

Nishizawa, Hitoshi; Handayaningsih, Anastasia Evi; Iguchi, Genzo; Cho, Yoshitake; Takahashi, Michiko; Yamamoto, Masaaki; Suda, Kentaro; Kasahara, Keiji; Hakuno, Fumihiko; Yamanouchi, Keitaro; Nishihara, Masugi; Seino, Susumu; Takahashi, Shinichiro; Takahashi, Yutaka

doi:10.1016/j.ghir.2012.02.001

Cited by 28 publications

(19 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Nishizawa et al [16] showed that increased IGF-1 is associated with increased oxidative stress both in rats and humans. Recently, Anagnostis et al [41] evaluated fifteen patients with acromegaly with age and sex matched controls and reported increased oxidative stress parameters and reduced antioxidant capacity as well as endothelial dysfunction evaluated with nitric oxide levels in acromegaly.…”

Section: Discussionmentioning

confidence: 99%

“…In the literature, a few studies have shown increased oxidative stress and reduced antioxidant status in acromegaly [16]. In contrast, the role of subclinical inflammation on atherosclerosis in acromegaly is still contradictory because previous studies have shown that patients with acromegaly primarily have decreased [17] as well as unchanged [18] high sensitive C-reactive protein (hs CRP) levels.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Markers of early atherosclerosis, oxidative stress and inflammation in patients with acromegaly

et al. 2014

View full text Add to dashboard Cite

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Markers of early atherosclerosis, oxidative stress and inflammation in patients with acromegaly

et al. 2014

View full text Add to dashboard Cite

show abstract

“…In addition, studies conducted on transgenic animal models exhibiting an excess production of GH found that these animals possessed higher rates of oxygen consumption and free radical production (Brown-Borg et al 2001;Rosa et al 2008). Significantly, serum markers of oxidative stress have also been shown to be elevated in acromegaly in humans (Nishizawa et al 2012).…”

Section: Introductionmentioning

confidence: 99%

The effect of recombinant human growth hormone and insulin-like growth factor-1 on the mitochondrial function and viability of peripheral blood mononuclear cells in vitro

Keane

Tajouri

Gray

2015

Appl. Physiol. Nutr. Metab.

View full text Add to dashboard Cite

This study investigated whether the putative physiological benefits induced by growth hormone (GH) and insulin-like growth factor-1 (IGF-1) are countered at supra-physiological concentrations because of an augmentation in the production of mitochondrial-derived free radicals with a subsequent increase in oxidative damage, compromising mitochondrial function. To test this hypothesis, peripheral blood mononuclear cells were incubated for 4 h with either recombinant human GH (rhGH) (range = 0.25-100 μg/L) or recombinant IGF-1 (rIGF-1) (range = 100-600 μg/L) and along with control samples were subsequently analyzed by flow cytometry for the determination of cellular viability, mitochondrial membrane potential (Δψm), mitochondrial superoxide (O2(-)) generation, and mitochondrial permeability transition pore (mtPTP) activity. Results showed levels of mitochondrial O2(-) generation to be significantly reduced compared with control samples (lymphocytes: 21.5 ± 1.6 AU; monocytes: 230.2 ± 9.8 AU) following rhGH treatment at both concentrations of 5 μg/L (13.5 ± 1.3 AU, P ≤ 0.05) and 10 μg/L (12.3 ± 1.5 AU, P ≤ 0.05) in lymphocytes and at 10 μg/L (153.4 ± 11.4 AU, P ≤ 0.05) in monocytes. However, no significant effect was found at either higher rhGH concentrations or following treatment with any concentration of rIGF-1. In addition, neither of the 2 hormones had any significant effect on Δψm, mtPTP activity, or on cellular viability. In conclusion, physiological concentrations of rhGH elicited a protective cellular effect through the reduction of oxidative free radicals within mitochondria. This antioxidant effect was diminished at supra-physiological concentrations but not to a level that would elicit disruption of mitochondrial function.

show abstract

“…Так, показано [1,2] значимое увели-чение основного маркера окислительного стресса -уровня ТБК у таких пациентов. Однако у пациентов с акромегалией не было обнаружено снижения ак-тивности КАТ (хотя наблюдалась тенденция к ее уменьшению) и уровня НТ.…”

Section: результаты и обсуждениеunclassified

The state of the blood antioxidant system in the patients presenting with acromegaly

Faassen¹,

Pankratova²,

Molitvoslovova³

et al. 2015

Probl Endokrinol (Mosk)

View full text Add to dashboard Cite

The state of the blood antioxidant system in the patients presenting with acromegaly. The objective of the present study was to evaluate the total antioxidant activity (TAA) of blood plasma, the levels of non-protein thiols and 2-thyobarbituric acid-active products (TBA-AP), superoxide dismutase (SOD) and catalase (CAT) activities as well as ceruloplasmin (CP) level in the patients presenting with acromegaly. It was shown that plasma TAA and SOD activity in this patients was on the average 20 and 30% lower respectively than in the control subjects. At the same time, the TBA-AP and CP levels increased by 50 and 40% respectively. These data suggest the development of oxidative stress in the acromegalic patients.

show abstract

Enhanced oxidative stress in GH-transgenic rat and acromegaly in humans

Cited by 28 publications

References 37 publications

Markers of early atherosclerosis, oxidative stress and inflammation in patients with acromegaly

Markers of early atherosclerosis, oxidative stress and inflammation in patients with acromegaly

The effect of recombinant human growth hormone and insulin-like growth factor-1 on the mitochondrial function and viability of peripheral blood mononuclear cells in vitro

The state of the blood antioxidant system in the patients presenting with acromegaly

Contact Info

Product

Resources

About