Enhanced oxidative-stress defense in transgenic potato expressing tomato Cu,Zn superoxide dismutases

Perl, Avihai; Perl‐Treves, Rafael; Galili, Shmuel; Aviv, D.; Shalgi, E.; Malkin, Shmuel; Galun, Esra

doi:10.1007/bf00220915

Cited by 207 publications

(106 citation statements)

References 44 publications

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“…Similarly, transgenic plants that overproduced superoxide dismutase that converts superoxide radicals (Oi-) into H,O, have been reported to have enhanced tolerance to photooxidative stress caused by paraquat or ozone (Bowler et al, 1991;Perl et al, 1993;Sen Gupta et al, 1993). We report here that suppression of the antioxidant enzyme GR with the antisense technique enhances sensitivity of plants to paraquat stress.…”

Section: Discussionmentioning

confidence: 56%

Decrease in Activity of Glutathione Reductase Enhances Paraquat Sensitivity in Transgenic Nicotiana tabacum

et al. 1995

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Section: Discussionmentioning

confidence: 56%

Decrease in Activity of Glutathione Reductase Enhances Paraquat Sensitivity in Transgenic Nicotiana tabacum

et al. 1995

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“…Such photooxidative damage was greatly decreased in transgenic tobacco plants that were capable of overexpressing genes encoding the chloroplastic CuZn-SOD enzymes (Sen Gupta et al, 1993a,b). Overexpression of CuZn-SOD genes was also demonstrated in plants exposed to herbicide treatment (Perl et al, 1993), sulphur dioxide (Madamanchi et al, 1994), UV-B radiation (Willekens et al, 1994 ;Rao et al, 1996) and pathogenic infection (Mittler et al, 1996 ;Fodor et al, 1997). As shown under field conditions, chilling stress in citrus and drought stress in wheat became more pronounced in plants suffering from Zn deficiency (Cakmak et al, 1995 ;Ekiz et al, 1998).…”

Section:       mentioning

confidence: 96%

Tansley Review No. 111

2000

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Zinc deficiency is one of the most widespread micronutrient deficiencies in plants and causes severe reductions in crop production. There are a number of physiological impairments in Zn-deficient cells causing inhibition of the growth, differentiation and development of plants. Increasing evidence indicates that oxidative damage to critical cell compounds resulting from attack by reactive O # species (ROS) is the basis of disturbances in plant growth caused by Zn deficiency. Zinc interferes with membrane-bound NADPH oxidase producing ROS. In Zn-deficient plants the iron concentration increases, which potentiates the production of free radicals. The Zn nutritional status of plants influences photooxidative damage to chloroplasts, catalysed by ROS. Zinc-deficient leaves are highly light-sensitive, rapidly becoming chlorotic and necrotic when exposed to high light intensity. Zinc plays critical roles in the defence system of cells against ROS, and thus represents an excellent protective agent against the oxidation of several vital cell components such as membrane lipids and proteins, chlorophyll, SH-containing enzymes and DNA. The cysteine, histidine and glutamate or aspartate residues represent the most critical Znbinding sites in enzymes, DNA-binding proteins (Zn-finger proteins) and membrane proteins. In addition, animal studies have shown that Zn is involved in inhibition of apoptosis (programmed cell death) which is preceded by DNA and membrane damage through reactions with ROS.

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“…Tuber discs (3 mm diameter) of Solanum tuberosum cv Desiree were co-cultivated with the recombinant Agrobacterium cells. Culture conditions were similar to those previously described by Perl et al (1991). Regenerated shoots were produced on Murashige and Skoog medium (Murashige and Skoog, 1962) supplemented with kanamycin (100 mg/L) and Claforan (500 mg/L), and the transformants were selected by rooting on kanamycin (100 mg/L) and Claforan (250 mg/L) Murashige and Skoog medium.…”

Section: Potato Transformation Regeneration and Growth Conditionsmentioning

confidence: 99%

Tissue-Specific Expression of the Tobacco Mosaic Virus Movement Protein in Transgenic Potato Plants Alters Plasmodesmal Function and Carbohydrate Partitioning

et al. 1996

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) were employed t o further explore the mode by which this viral protein interacts with cellular metabolism t o change carbohydrate allocation. Dye-coupling experiments established that expression of the TMV-MP alters plasmodesmal function in both potato leaves and tubers when expressed in the respective tissues. However, whereas the sizeexclusion limit of mesophyll plasmodesmata was increased to a value greater than 9.4 kD, this size limit was smaller for plasmodesmata interconnecting tuber parenchyma cells. Starch and sugars accumulated in potato leaves t o significantly lower levels in plants expressing the TMV-MP under the ST-LS1 promoter, and rate of sucrose efflux from petioles of the latter was higher compared to controls. It is interesting that this effect was expressed only in mature plants after tuber initiation. No effect on carbohydrate levels was found in plants expressing this protein under the 833 promoter. These results are discussed in terms of the mode by which the TMV-MP exerts its influence over carbon metabolism and photoassimilate translocation, and the possible role of plasmodesmal function i n controlling these processes.It is generally accepted that most if not a11 viruses move from cell to cell via plasmodesmata. This process requires an interaction between a specific virally encoded protein, termed the MP, and proteins within the plasmodesmata of the host plant . Expression of the TMV-MP in transgenic tobacco plants provided the first evidence that this protein potentiates the short-distance transport of viral infectious material (Deom et al., 1987 Immunolocalization studies indicated that the TMV-MP was localized mainly in secondary plasmodesmata connecting mesophyll cells and bundle-sheath cells to phloem parenchyma cells (Ding et al., 1992). Dye-coupling studies established that the SEL of plasmodesmata interconnecting the mesophyll cells of these transgenic plants was greater than 9.4 kD, as compared to 800 D in control plants, indicating that the TMV-MP has a direct effect on plasmodesmal function (Wolf et al., 1989). Based on plasmodesmal frequencies and dye-coupling experiments, it has been assumed that plasmodesmata play an important role in regulating symplasmic transport (Robards and Lucas, 1990). Pursuant to this assumption, transgenic plants in which plasmodesmata are modified provide an elegant system in which to further test the hypothesis that diffusion of SUC through plasmodesmata acts as a limiting step to symplasmic transport. Our comparative analysis of leaf photosynthetic performance, carbohydrate level, and carbon export in TMV-MP transgenic and control tobacco plants revealed a complex influence of the MP over these parameters (Lucas et al., 1993b;Olesinski et al., 1995). Fully expanded leaves of transgenic tobacco plants expressing the TMV-MP accumulated much higher levels of SUC, Glc, Fru, and starch during the day than did those of control plants. Direct measurements of 14C-photosynthate translocation from source leaves indicated that export was lower in plan...

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Enhanced oxidative-stress defense in transgenic potato expressing tomato Cu,Zn superoxide dismutases

Cited by 207 publications

References 44 publications

Decrease in Activity of Glutathione Reductase Enhances Paraquat Sensitivity in Transgenic Nicotiana tabacum

Decrease in Activity of Glutathione Reductase Enhances Paraquat Sensitivity in Transgenic Nicotiana tabacum

Tansley Review No. 111

Tissue-Specific Expression of the Tobacco Mosaic Virus Movement Protein in Transgenic Potato Plants Alters Plasmodesmal Function and Carbohydrate Partitioning

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