Enhanced osteogenic differentiation of mesenchymal stem cells on poly(<scp>l</scp>-lactide) nanofibrous scaffolds containing carbon nanomaterials

Duan, Shun; Yang, Xiaoping; Mei, Fang; Tang, Yue; Li, Xiaoli; Shi, Yuzhou; Mao, Jifu; Zhang, Hongquan; Cai, Qing

doi:10.1002/jbm.a.35283

Cited by 83 publications

(71 citation statements)

References 43 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The results suggested that the high pre-concentration capacity of the GO/ PLL fi lms for osteogenic inducers could play an important part in the signifi cant enhancement of osteogenic differentiation of MSCs. Similarly, poly(L-lactide) (PLLA) nanofi brous scaffolds containing carbon nanomaterials, such as CNT and graphene were fabricated by Duan et al [ 136 ] In a recent study, Tatavarty et al synthesized nanocomposites consisting of oblong ultrathin plate shaped calcium phosphate (CaP) nanoparticles and GO microfl akes. [ 137 ] The GO-CaP nanocomposites signifi cantly facilitated the osteogenesis of hMSCs and further enhanced calcium deposition.…”

Section: Methodsmentioning

confidence: 98%

Graphene‐Based Materials in Regenerative Medicine

Ding

Liu

Fan

2015

Adv Healthcare Materials

143

100

View full text Add to dashboard Cite

Graphene possesses many unique properties such as two-dimensional planar structure, super conductivity, chemical and mechanical stability, large surface area, and good biocompatibility. In the past few years, graphene-based materials have risen as a shining star on the path of researchers seeking new materials for future regenerative medicine. Herein, the recent research advances made in graphene-based materials mostly utilizing the mechanical and electrical properties of graphene are described. The most exciting findings addressing the impact of graphene-based materials on regenerative medicine are highlighted, with particular emphasis on their applications including nerve, bone, cartilage, skeletal muscle, cardiac, skin, adipose tissue regeneration, and their effects on the induced pluripotent stem cells. Future perspectives and emerging challenges are also addressed in this Review article.

show abstract

Section: Methodsmentioning

confidence: 98%

Graphene‐Based Materials in Regenerative Medicine

Ding

Liu

Fan

2015

Adv Healthcare Materials

143

100

View full text Add to dashboard Cite

show abstract

“…This can be attributed to the increase in specific surface area of the scaffold and the surface roughness that can increase adsorption of proteins. Furthermore, graphene provides more contacting surface to cells as compared to the same content of CNT [80]. …”

Section: Combining Graphene and Various Materials To Enhance Osteomentioning

confidence: 99%

Graphene: A Versatile Carbon-Based Material for Bone Tissue Engineering

Dubey

Bentini

Islam

et al. 2015

Stem Cells International

193

135

View full text Add to dashboard Cite

The development of materials and strategies that can influence stem cell attachment, proliferation, and differentiation towards osteoblasts is of high interest to promote faster healing and reconstructions of large bone defects. Graphene and its derivatives (graphene oxide and reduced graphene oxide) have received increasing attention for biomedical applications as they present remarkable properties such as high surface area, high mechanical strength, and ease of functionalization. These biocompatible carbon-based materials can induce and sustain stem cell growth and differentiation into various lineages. Furthermore, graphene has the ability to promote and enhance osteogenic differentiation making it an interesting material for bone regeneration research. This paper will review the important advances in the ability of graphene and its related forms to induce stem cells differentiation into osteogenic lineages.

show abstract

“…The nanofibrous structure itself demonstrated significant enhancement in cell adhesion, proliferation and oseogenic differentiation of bone mesenchymal stem cells, and with the incorporation of carbon nanomaterials. 26 Therefore, ExCNF also has osteoinductive properties and induced bone healing.…”

Section: Effects Of Exfoliated Carbon Nanofibers In a Rat Femoral Framentioning

confidence: 99%

Enhancement of the Effects of Exfoliated Carbon Nanofibers by Bone Morphogenetic Protein in a Rat Femoral Fracture Model

Miyazaki

Toyoda

Yoshiiwa

et al. 2014

Journal Orthopaedic Research

View full text Add to dashboard Cite

Exfoliated carbon nanofibers (ExCNFs) are expected to serve as excellent scaffolds for promoting and guiding bone-tissue regeneration. We aimed to enhance the effects of ExCNFs with bone morphogenetic proteins (BMPs) and examine their feasibility and safety in clinical applications using a rat femoral fracture model. Group I (n ¼ 16) animals were implanted with control MedGEL. Group II (n ¼ 17) animals were implanted with MedGEL containing ExCNFs. Group III (n ¼ 15) animals were implanted with MedGEL containing 1 mg rhBMP-2. Group IV (n ¼ 15) animals were implanted with MedGEL containing 1 mg rhBMP-2 and ExCNFs. The rats were euthanized after 6 weeks, and their fractured femurs were explanted and assessed by manual palpation, radiographs, and highresolution microcomputerized tomography (micro-CT); the femurs were also subjected to biomechanical and histological analysis. The fusion rates in Group IV (73.3%) were considerably higher than those in Groups I (25. Keywords: exfoliated carbon nanofiber; bone morphogenetic protein; fracture healing; rat femoral fracture model; scaffold Bone morphogenetic proteins (BMPs) are members of the transforming growth factor b superfamily and are powerful osteoinductive molecules. The osteoinductive effects of recombinant human BMPs (rhBMPs) in surgical procedures such as fracture repair, healing of critical-size bone defects, and spinal fusion have been demonstrated in animal models and clinical trials; furthermore, rhBMP-2 and rhBMP-7 have been approved for clinical use. [1][2][3][4][5] Unfortunately, the results of clinical trials show that high doses of BMPs are required to induce adequate bone fusion, as the molecules are soluble and easily diffuse away from the fusion site, becoming inactivated in vivo. 6 In addition, the usefulness of BMPs is limited by their high costs. To address these problems, a number of strategies are being developed to generate safer, less expensive, and more efficacious spinal fusion using rhBMP. However, the efficient immobilization of BMPs onto carriers at low dosage while maintaining desirable in vivo regenerative action remains as a major challenge in bone regeneration research and practice. 7,8 The required rhBMP-2 level in humans, even for the FDA-approved collagen sponge-incorporated treatment, is much higher (over six orders of magnitude greater) than that used for in vitro cell level evaluation and in vivo animal studies. 7,9 Several ongoing investigations aim to develop a scaffold that functions as a delivery vehicle, providing cells with a more controlled and sustained release of bioactive molecules to maintain the local growth factor concentration within the therapeutic range.Carbon nanotubes (CNTs) have received much attention because of their interesting properties, including their exceptional mechanical 10 and high electrical and thermal conductivity, 11 which facilitate their use as reinforcements or additives in materials such as plastics, metals, and ceramics to improve the properties of the materials and introduce nove...

show abstract

Enhanced osteogenic differentiation of mesenchymal stem cells on poly(l-lactide) nanofibrous scaffolds containing carbon nanomaterials

Cited by 83 publications

References 43 publications

Graphene‐Based Materials in Regenerative Medicine

Graphene‐Based Materials in Regenerative Medicine

Graphene: A Versatile Carbon-Based Material for Bone Tissue Engineering

Enhancement of the Effects of Exfoliated Carbon Nanofibers by Bone Morphogenetic Protein in a Rat Femoral Fracture Model

Contact Info

Product

Resources

About