Enhanced osteogenesis by collagen‐binding peptide from bone sialoprotein in vitro and in vivo

Abstract

Bone sialoprotein (BSP) is a mineralized, tissue-specific, and noncollagenous protein. The binding of BSP to collagen is thought to be important for the initiation of bone mineralization and formation. In this study, we elucidated the osteogenic efficiency of the collagen-binding (CB) peptide derived from BSP in vitro and in vivo. The CB peptide increased osteoblastic differentiation marker gene and protein expression without affecting cell proliferation. The osteoblastic differentiation by the CB peptide is performed by the activation of extracellular signal-regulated kinase (ERK1/2) and protein kinase B (Akt). Notably, the activation of CB peptide-induced osteogenic differentiation was completely blocked to the basal level by the specific inhibitors for ERK1/2 (U0126) and Akt (LY294002). In vivo results further demonstrated that the CB peptide-coated hydroxyapatite scaffold was able to induce bone formation in the bone defect. Taken together, the CB peptide can be developed as an osteoblastic differentiation agent as well as a fusion biomaterial for bone regeneration therapy.

Identification of buffalo casein-derived bioactive peptides with osteoblast proliferation activity

“…Epidemiological studies reported that milk is a well-known bone health-promoting agent [12,13]. As the osteoblast proliferation is crucial, part of the bone formation or remodeling osteoblast proliferation effect is used for screening osteogenic bioactive peptides [28,31,[33][34][35].…”

“…1). MTT is a simple reliable method to evaluate osteoblast proliferation [27][28][29][30][31]; hence, the proliferation effect of PT hydrolysates and peptides was evaluated by MTT method. At lower concentration, PT hydrolysates did not show any proliferation effect, but it started inducing proliferation of calvarial cells at 50 µg/ ml, and the proliferation effect remained constant up to 100 µg/ml.…”

Adsorbed fibrinogen leads to improved bone regeneration and correlates with differences in the systemic immune response

“…antiresorptive or osteogenic), is one of the central strategies being explored for bone repair [3,4,11]. However, promotion of bone regeneration through inflammatory signals remains largely unexplored, in spite of evidence that inflammation may have a positive role in bone remodeling.…”

“…Moreover, the role of immune cells and inflammatory mediators in biomaterial-mediated bone repair/regeneration has been considerably overlooked. It is not uncommon to find studies reporting the absence of (or mild) inflammatory reactions, associated with degradable biomaterials developed for tissue repair/regeneration [3,4].…”

Synergistic influence of collagen I and BMP 2 drives osteogenic differentiation of mesenchymal stem cells: A cell microarray analysis

“…The differentiation of multipotent MSCs into osteogenic cells depends on the spatially coordinated activities of multiple signalling pathways that include growth factors (GFs) and extracellular matrix (ECM) components [7][8][9]. Considerable attention has been devoted to extrinsic factors that orchestrate determination and differentiation of MSCs [10][11][12][13][14].…”