Enhanced osteoconductivity of micro‐structured titanium implants (XiVE S CELLplus<sup>™</sup>) by addition of surface calcium chemistry: a histomorphometric study in the rabbit femur

Park, Jin Woo; Kim, Hye‐Kyoung; Kim, Youn-Jeong; An, Chang-Hyeon; Hanawa, Takao

doi:10.1111/j.1600-0501.2009.01714.x

Cited by 49 publications

(59 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…192 This method also caused incorporation of calcium as CaTiO 3 in the protective surface oxide layer. Titanium and Ti6Al4V implants with thus treated surfaces significantly increased BIC and removal torque forces in rabbit tibiae.…”

Section: In Vivo and Clinical Applicationsmentioning

confidence: 99%

Nanoscale surface modifications of medically relevant metals: state-of-the art and perspectives

et al. 2011

View full text Add to dashboard Cite

Evidence that nanoscale surface properties stimulate and guide various molecular and biological processes at the implant/tissue interface is fostering a new trend in designing implantable metals. Cutting-edge expertise and techniques drawn from widely separated fields, such as nanotechnology, materials engineering and biology, have been advantageously exploited to nanoengineer surfaces in ways that control and direct these processes in predictable manners. In this review, we present and discuss the state-of-the-art of nanotechnology-based approaches currently used to modify the surface of metals used for orthopedic and dental applications, and also briefly consider their use in the cardiovascular field. The effects of nanoengineered surfaces on various in vitro molecular and cellular events are firstly discussed. Importantly, this review also provides an overview of in vivo and clinical studies with nanostructured metallic implants, and addresses the potential influence of nanotopography on biomechanical events at interfaces. Ultimately the objective of this work is to give the readership a comprehensive picture of the current advances, future developments and challenges in the application of the infinitesimally small to biomedical surface science. We believe that an integrated understanding of the in vitro and particularly of the in vivo behavior is mandatory for the proper exploitation of nanostructured implantable metals and, as a matter of fact, all biomaterials.

show abstract

Section: In Vivo and Clinical Applicationsmentioning

confidence: 99%

Nanoscale surface modifications of medically relevant metals: state-of-the art and perspectives

et al. 2011

View full text Add to dashboard Cite

show abstract

“…Several studies have reported important contributions from surface chemistry and micro-topography on osteogenesis [69][70][71][72][73][74] and Scaglione, Braccini, Wendt, Jaquiery, Beltrame, Quarto, and Martin in 2006 demonstrated osteogenesis on a 3D ceramic scaffold in the absence of defined osteo-inductive molecules [75]. This together Fig.…”

Section: Discussionmentioning

confidence: 70%

Delivery of definable number of drug or growth factor loaded poly(dl-lactic acid-co-glycolic acid) microparticles within human embryonic stem cell derived aggregates

Qutachi

Shakesheff

Buttery

2013

Journal of Controlled Release

View full text Add to dashboard Cite

Embryoid bodies (EBs) generated from embryonic stem cells are used to study processes of differentiation within a three dimensional (3D) cell environment. In many instances however, EBs are dispersed to single cell suspensions with a subsequent monolayer culture. Moreover, where the 3D integrity of an EB is maintained, cytokines or drugs of interest to stimulate differentiation are often added directly to the culture medium at fixed concentrations and effects are usually limited to the outer layers of the EB. The aim of this study was to create an EB model with localised drug and or growth factor delivery directly within the EB. Using poly(DL-lactic acid-co-glycolic acid) microparticles (MPs) with an average diameter of 13μm, we have demonstrated controllable incorporation of defined numbers of MPs within human ES cell derived EBs, down to 1 MP per EB. This was achieved by coating MPs with human ES cell lysate and centrifugation of specific ratios of ES cells and MPs to form 3D aggregates. Using MPs loaded with simvastatin (pro or active drug) or BMP-2, we have demonstrated osteogenic differentiation within the 3D aggregates, maintained in culture for up to 21days, and quantified by real time QPCR for osteocalcin. Immunostaining for RUNX2 and osteocalcin, and also histochemical staining with picrosirius red to demonstrate collage type 1 and Alizarin red to demonstrate calcium/mineralisation further demonstrated osteogenic differentiation and revealed regional staining associated with the locations of MPs within the aggregates. We also demonstrated endothelial differentiation within human ES cell-derived aggregates using VEGF loaded MPs. In conclusion, we demonstrate an effective and reliable approach for engineering stem aggregates with definable number of MPs within the 3D cellular structure. We also achieved localised osteogenic and endothelial differentiation associated with MPs releasing encapsulated drug molecules or cytokines directly within the cell aggregate. This provides a powerful tool for controlling and investigating differentiation within 3D cell cultures and has applications to drug delivery, drug discovery, stem cell biology, tissue engineering and regenerative medicine.

show abstract

“…Gao et al fabricated a micro/nanostructured porous surface on titanium and observed increased hydroxyapatite formation [32], and Kubo [14] and Zinger [33] found that microscale and nanoscale topography of the implant surface exhibited a synergistic effect on bone marrow osteoblasts and human-bone-derived MG63 cells proliferation. Furthermore, Park et al showed that the nanostructured Ca-incorporated oxide layer produced by hydrothermal treatment on microstructured titanium implants surface was effective in improving the osseointegration of implants in areas of loose cancellous bone [34].…”

Section: Discussionmentioning

confidence: 99%

The effect of hierarchical micro/nanosurface titanium implant on osseointegration in ovariectomized sheep

et al. 2010

View full text Add to dashboard Cite

show abstract

Enhanced osteoconductivity of micro‐structured titanium implants (XiVE S CELLplus^™) by addition of surface calcium chemistry: a histomorphometric study in the rabbit femur

Cited by 49 publications

References 37 publications

Nanoscale surface modifications of medically relevant metals: state-of-the art and perspectives

Nanoscale surface modifications of medically relevant metals: state-of-the art and perspectives

Delivery of definable number of drug or growth factor loaded poly(dl-lactic acid-co-glycolic acid) microparticles within human embryonic stem cell derived aggregates

The effect of hierarchical micro/nanosurface titanium implant on osseointegration in ovariectomized sheep

Contact Info

Product

Resources

About

Enhanced osteoconductivity of micro‐structured titanium implants (XiVE S CELLplus™) by addition of surface calcium chemistry: a histomorphometric study in the rabbit femur

Cited by 49 publications

References 37 publications

Nanoscale surface modifications of medically relevant metals: state-of-the art and perspectives

Nanoscale surface modifications of medically relevant metals: state-of-the art and perspectives

Delivery of definable number of drug or growth factor loaded poly(dl-lactic acid-co-glycolic acid) microparticles within human embryonic stem cell derived aggregates

The effect of hierarchical micro/nanosurface titanium implant on osseointegration in ovariectomized sheep

Contact Info

Product

Resources

About

Enhanced osteoconductivity of micro‐structured titanium implants (XiVE S CELLplus^™) by addition of surface calcium chemistry: a histomorphometric study in the rabbit femur