Enhanced of α-Glucosidase Production by Saccharomyces cerevisiae C8-5 and Candida tropicalis C0-7, Two Yeast Strains Isolated from Tchapalo, a Traditional Sorghum Beer of Côte d’Ivoire

Current advances in Candida tropicalis: Yeast overview and biotechnological applications

“…A sorghum beer C. tropicalis isolate was used to produce α‐glucosidase in shake flasks from a semi‐defined medium containing corn starch 1% as the carbon source. Maximum α‐glucosidase production (8.15 U/mL) was achieved after 48 h fermentation at 150 rpm, pH 7.0, and 37°C 88 . When this strain was cocultured with S. cerevisiae at a 1:1 cell ratio, α‐glucosidase production from corn starch 1% reached 9.14 U/mL after 48 h fermentation at pH 7.0, 150 rpm, and 30°C 89 .…”

“…A sorghum beer C. tropicalis isolate was used to produce α-glucosidase in shake flasks from a semi-defined medium containing corn starch 1% as the carbon source. Maximum α-glucosidase production (8.15 U/mL) was achieved after 48 h fermentation at 150 rpm, pH 7.0, and 37 • C. 88 When this strain was cocultured with S. cerevisiae at a 1:1 cell ratio, α-glucosidase production from corn starch 1% reached 9.14 U/mL after 48 h fermentation at pH 7.0, 150 rpm, and 30 • C. 89 Another enzyme that can be produced by C. tropicalis is xylose reductase, an intracellular enzyme that catalyzes the first reaction of xylose metabolism: xylose-to-xylitol conversion with concomitant oxidation of NAD(P)H. This enzyme may have potential applications in the production of some bioproducts, such as xylitol, sorbitol, and ethanol. C. tropicalis IFO 0618 strain was used to produce xylose reductase from Meranti wood sawdust hemicellulosic hydrolysate supplemented with yeast extract and minerals in shake flasks at pH 6.0, 30 • C, and 150 rpm for 24 h. A crude xylose reductase with 11.16 U/mL activity was obtained.…”