Enhanced Ocular Anti-Inflammatory Activity of Ibuprofen Carried by an Eudragit RS100&lt;sup&gt;®&lt;/sup&gt; Nanoparticle Suspension

Bucolo, Claudio; Maltese, Adriana; Puglisi, Giovanni; Pignatello, Rosario

doi:10.1159/000065608

Cited by 78 publications

(47 citation statements)

References 10 publications

(15 reference statements)

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…It was suggested that the components forming the nanocapsules act as penetration enhancers or as an endocytotic stimulator. The investigation of the ocular pharmacodynamic profile of ibuprofen-loaded Eudragit nanoparticles also leads to the conclusion that the nanosystem increased the bioavailability of the anti-inflammatory drug and ultimately its pharmacological activity (359).…”

Section: Inflammationmentioning

confidence: 99%

Nanotechnology: Intelligent Design to Treat Complex Disease

2006

View full text Add to dashboard Cite

The purpose of this expert review is to discuss the impact of nanotechnology in the treatment of the major health threats including cancer, infections, metabolic diseases, autoimmune diseases, and inflammations. Indeed, during the past 30 years, the explosive growth of nanotechnology has burst into challenging innovations in pharmacology, the main input being the ability to perform temporal and spatial site-specific delivery. This has led to some marketed compounds through the last decade. Although the introduction of nanotechnology obviously permitted to step over numerous milestones toward the development of the "magic bullet" proposed a century ago by the immunologist Paul Ehrlich, there are, however, unresolved delivery problems to be still addressed. These scientific and technological locks are discussed along this review together with an analysis of the current situation concerning the industrial development.

show abstract

Section: Inflammationmentioning

confidence: 99%

Nanotechnology: Intelligent Design to Treat Complex Disease

2006

View full text Add to dashboard Cite

show abstract

“…62 Some studies discussed immune system stimulation by methacrylate nanoparticles. 20,[62][63][64][65][66] Although IL-12 stimulation by nanostructures without peptide has been observed (Figure 4), this same stimulus was not observed when nanostructures were associated with clavanin, probably because clavanin A suppresses the effect as shown in Figure 4. Furthermore, it is important to report that besides IL-12 stimulation by nanostructures without peptide, no cytotoxic effects against erythrocytes were observed.…”

Section: Immunologic In Vitro Assaysmentioning

confidence: 99%

Clavanin bacterial sepsis control using a novel methacrylate nanocarrier

Saúde¹,

Ombredane²,

Silva³

et al. 2014

IJN

View full text Add to dashboard Cite

Controlling human pathogenic bacteria is a worldwide problem due to increasing bacterial resistance. This has prompted a number of studies investigating peptides isolated from marine animals as a possible alternative for control of human pathogen infections. Clavanins are antimicrobial peptides isolated from the marine tunicate Styela clava , showing 23 amino acid residues in length, cationic properties, and also high bactericidal activity. In spite of clear benefits from the use of peptides, currently 95% of peptide properties have limited pharmaceutical applicability, such as low solubility and short half-life in the circulatory system. Here, nanobiotechnology was used to encapsulate clavanin A in order to develop nanoantibiotics against bacterial sepsis. Clavanin was nanostructured using EUDRAGIT ® L 100-55 and RS 30 D solution (3:1 w:w). Atomic force, scanning electron microscopy and dynamic light scattering showed nanoparticles ranging from 120 to 372 nm in diameter, with a zeta potential of -7.16 mV and a polydispersity index of 0.123. Encapsulation rate of 98% was assessed by reversed-phase chromatography. In vitro bioassays showed that the nanostructured clavanin was partially able to control development of Staphylococcus aureus , Klebsiella pneumoniae , and Pseudomonas aeruginosa . Furthermore, nanostructures did not show hemolytic activity. In vivo sepsis bioassays were performed using C57BL6 mice strain inoculated with a polymicrobial suspension. Assays led to 100% survival rate under sub-lethal sepsis assays and 40% under lethal sepsis assays in the presence of nanoformulated clavanin A until the seventh day of the experiment. Data here reported indicated that nanostructured clavanin A form shows improved antimicrobial activity and has the potential to be used to treat polymicrobial infections.

show abstract

“…71 In an in vivo study using rabbit eye, EUDRAGIT nanosuspension containing 0.1% ibuprofen showed a very good ocular tolerability. 33,72 It is useful to understand anatomical differences between species for interpreting toxicological and pathological responses to the eye and for human risk assessment of these new therapies.…”

Section: Cytotoxicity and Toxicological Concernsmentioning

confidence: 99%