Enhanced nonenzymatic sensing of hydrogen peroxide released from living cells based on Fe₃O₄/self-reduced graphene nanocomposites

Abstract

A novel, sensitive, nonenzymatic amperometric sensor for the reliable detection of extracellular H 2 O 2 released from living cells was fabricated based on Fe 3 O 4 /reduced graphene oxide (Fe 3 O 4 /rGO) nanocomposites, which were prepared using a simple and cost-effective one-pot approach. Electrochemical performances of the Fe 3 O 4 /rGO nanocomposites modified glassy carbon electrode (GCE) were studied. The results demonstrated that this H 2 O 2 sensor exhibited excellent electrocatalytic performance towards the reduction of H 2 O 2 at a potential of À0.3 V in the wide linear concentration range from 0.001 to 20 mM with a high sensitivity of 387.6 mA mM À1 cm À2 and a detection limit as low as 0.17 mM (S/N ¼ 3), which was lower than certain enzymes and noble metal nanomaterials-based biosensors. Moreover, good anti-interference property, reproducibility, and long-term stability of the enzymeless sensor were achieved. Because of these remarkable analytical advantages, a novel, effective approach for the detection of extracellular H 2 O 2 released from HeLa cells stimulated by CdTe quantum dots (QDs) was established by the constructed sensor. Since H 2 O 2 is a byproduct of several oxidative biological reactions, this work could be applied to study the downstream biological effects of various stimuli in pathophysiology, and may expand the application of Fe 3 O 4 -based nanomaterials in the field of electrochemical sensing and bioanalysis.

SnFe2O4 Nanocrystals as Highly Efficient Catalysts for Hydrogen‐Peroxide Sensing

“…Spinel oxides, with a general molecular formula of A 2+ B 3+ 2 O 2− 4 , find a wide range of applications in lithium‐ion batteries, photodegradation, and hydrogen generation . They have also been applied to H 2 O 2 sensing as the sensing catalyst, for example, FeFe 2 O 4 and CoCo 2 O 4 . Although performing well, there is still ample room for improvement.…”

“…[18][19][20] They have also been appliedto H 2 O 2 sensing as the sensing catalyst,f or example,F eFe 2 O 4 and CoCo 2 O 4 . [21][22][23][24] Although performing well, there is stilla mple room for improvement. SnFe 2 O 4 ,r eceiving relatively much lessr esearch attention in the past, was recently demonstrated to possess good photocatalytice fficiencies and showsa no utstandinga bility in photocatalyticd egradationo fR hB in Fenton-like processes, exhibiting excellent decomposition efficiencies of H 2 O 2 .…”

Nanoporous Carbon Nanofibers Decorated with Platinum Nanoparticles for Non-Enzymatic Electrochemical Sensing of H2O2

“…Recently, the electrochemical biosensors have attracted more and more attention for their excellent performance in both basic and applied studies [ 1 , 2 , 3 ]. Among all its applications, the analytical determination of hydrogen peroxide (H 2 O 2 ) is an emerging field [ 4 , 5 , 6 ]. Some techniques, such as spectrophotometry [ 7 ], chemiluminescence [ 8 ], and electrochemistry [ 9 , 10 ] have been employed previously to detect H 2 O 2 .…”

Fe3O4 nanoparticles on graphene oxide sheets for isolation and ultrasensitive amperometric detection of cancer biomarker proteins

“…Peroxidase-like activity of Fe 3 O 4 nanoparticles for electrochemical detection of hydrogen peroxide has been enhanced by incorporation with other materials like platinum (Ma et al, 2013), graphene derivatives (Fang et al, 2014; Liu et al, 2011; Yang et al, 2014), platinum/palladium (Sun et al, 2012), and gold (Sun et al, 2013). Chitosan coated Fe 3 O 4 nanoparticles were used in colorimetric ELISA to detect of carcinoembryonic antigen with 1 ng/mL LOD (Gao et al, 2008) and thrombin with LOD of 1 nM (Zhang et al, 2010).…”