Enhanced neuronal differentiation of neural stem cells with mechanically enhanced touch-spun nanofibrous scaffolds

Asheghali, Darya; Lee, Se‐Jun; Furchner, Andreas; Gruzd, Alexey; Larson, Steven R.; Tokarev, Alexander; Stake, Seth; Zhou, Xuan; Hinrichs, Karsten; Zhang, Lijie Grace; Minko, Sergiy

doi:10.1016/j.nano.2020.102152

Cited by 16 publications

(21 citation statements)

References 48 publications

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“…The solvent evaporates, and the fluid solidifies to form a fiber. The fiber diameter is governed Small 2020, 16,1907422 by the droplet falling distance, polymer solution surface tension, density, viscosity, and the volumetric evaporation rate per unit area of fluid stream surface, which further depends on the cross-sectional area exposed to air. The analysis of all those factors on the fiber characteristics is the subject of special studies, and it will be published elsewhere.…”

Section: Doi: 101002/smll201907422mentioning

confidence: 99%

“…Outside this cell-specific microenvironment, cell functions could be alternated, as shown in numerous experiments. Obviously, biomimetic 3D ECM is the most efficient approach for tissue regeneration and the study of cell response to their Small 2020, 16,1907422 microenvironment. GFD, in combination with additive manufacturing technology, offers a unique capability to mix fibers of different diameters at controlled spacing and orientation into the scaffold, thereby closely mimicking the natural ECM structure for cell differentiation, proliferation, and tissue development.…”

Section: Doi: 101002/smll201907422mentioning

confidence: 99%

“…Hence, 99% of unoccupied space in the scaffolds is available for cell diffusion, nutrient and metabolite exchange, and cell growth. RAW 264.7 macrophages Small 2020, 16,1907422 Figure 2. I) 2D arrays of PCL nanofibers: a) precisely aligned 2D arrays of nanofibers on the collection frame (inset); b) SEM image of a single nanofiber with a 500 nm diameter.…”

Section: Doi: 101002/smll201907422mentioning

confidence: 99%

“…[13] Despite its simplicity, efficiency, and scalability, the conventional ES and other rotating spinneret methods are not suitable to fabricate precisely aligned nanofibers into 3D scaffolds with controlled spacing and orientation because of instabilities of liquid jet.The effect of instabilities could be minimized by minimizing the distance between the injecting (e.g., nozzle) and fiber collecting (e.g., electrode) modules to as small as few millimeters. This approach was realized in different variants of ES, such as Small 2020, 16, 1907422…”

mentioning

confidence: 99%

“…The effect of instabilities could be minimized by minimizing the distance between the injecting (e.g., nozzle) and fiber collecting (e.g., electrode) modules to as small as few millimeters. This approach was realized in different variants of ES, such as Small 2020, 16, 1907422…”

mentioning

confidence: 99%

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Gravity Drawing of Micro‐ and Nanofibers for Additive Manufacturing of Well‐Organized 3D‐Nanostructured Scaffolds

Yadavalli

Asheghali

Tokarev

et al. 2020

Small

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This work introduces a gravity fiber drawing (GFD) method of making single filament nanofibers from polymer solutions and precise alignment of the fibers in 3D scaffolds. This method is advantageous for nanofiber 3D alignment in contrast to other known methods. GFD provides a technology for the fabrication of freestanding filament nanofibers of well‐controlled diameter, draw ratio, and 3D organization with controllable spacing and angular orientation between nanofibers. The GFD method is capable of fabricating complex 3D scaffolds combining fibers with different diameters, chemical compositions, mechanical properties, angular orientations, and multilayer structures in the same construct. The scaffold porosity can be as high as 99% to secure transport of nutrients and space for cell infiltration and differentiation in tissue engineering and 3D cell culture applications.

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Section: Doi: 101002/smll201907422mentioning

confidence: 99%

Section: Doi: 101002/smll201907422mentioning

confidence: 99%

Section: Doi: 101002/smll201907422mentioning

confidence: 99%

mentioning

confidence: 99%

mentioning

confidence: 99%

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Gravity Drawing of Micro‐ and Nanofibers for Additive Manufacturing of Well‐Organized 3D‐Nanostructured Scaffolds

Yadavalli

Asheghali

Tokarev

et al. 2020

Small

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Biofabrication of Composite Bioink‐Nanofiber Constructs: Effect of Rheological Properties of Bioinks on 3D (Bio)Printing and Cells Interaction with Aligned Touch Spun Nanofibers

Kitana,

Levario‐Diaz,

Cavalcanti‐Adam

et al. 2023

Adv Healthcare Materials

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This paper reports on a novel approach for the fabrication of composite multilayered bioink‐nanofibers construct. This work achieves this by using a hands‐free 3D (bio)printing integrated touch‐spinning approach. Additionally, this work investigates the interaction of fibroblasts in different bioinks with the highly aligned touch‐spun nanofibers. This work conducts a comprehensive characterization of the rheological properties of the inks, starting with low‐strain oscillatory rheology to analyze the viscoelastic behavior, when the material structure remains intact. Moreover, this work performs amplitude sweeps to investigate the stability of the inks under large deformations, rotational rheology to examine the shear thinning profile, and a three‐step creep experiment to study time‐dependent rheological behavior. The obtained rheological results are correlated to visual observation of the flow behavior of inks. These behaviors span from an ink with zero‐shear viscosity, very weak shear thinning, and no thixotropic behavior to inks exhibiting flow stress, pronounced shear thinning, and thixotropy. It is demonstrated that inks have an essential effect on cell behavior. While all bioinks allow a preferred directionality of the fibroblasts along the fiber direction, cells tend to form aggregates in bioinks with higher viscosity, and a considerable number of agglomerates are observed in the presence of laponite‐RD.

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Fibrous Scaffolds for Muscle Tissue Engineering Based on Touch‐Spun Poly(Ester‐Urethane) Elastomer

Uribe-Gomez

Schönfeld

Posada-Murcia

et al. 2022

Macromolecular Bioscience

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Development of fiber‐spinning technologies and materials with proper mechanical properties is highly important for the manufacturing of aligned fibrous scaffolds mimicking structure of the muscle tissues. Here, the authors report touch spinning of a thermoplastic poly(1,4‐butylene adipate)‐based polyurethane elastomer, obtained via solvent‐free polymerization. This polymer possesses a combination of important advantages such as 1) low elastic modulus in the range of a few MPa, 2) good recovery ratio and 3) resilience, 4) processability, 5) nontoxicity, 6) biocompatibility, and 7) biodegradability that makes it suitable for fabrication of structures mimicking extracellular matrix of muscle tissue. Touch spinning allows fast and precise deposition of highly aligned micro‐ and nano‐fibers without use of high voltage. C2C12 myoblasts readily align along soft polymer fibers and demonstrate high viability as well as proliferation that make proposed combination of polymer and fabrication method highly suitable for engineering skeletal muscles.

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Enhanced neuronal differentiation of neural stem cells with mechanically enhanced touch-spun nanofibrous scaffolds

Cited by 16 publications

References 48 publications

Gravity Drawing of Micro‐ and Nanofibers for Additive Manufacturing of Well‐Organized 3D‐Nanostructured Scaffolds

Gravity Drawing of Micro‐ and Nanofibers for Additive Manufacturing of Well‐Organized 3D‐Nanostructured Scaffolds

Biofabrication of Composite Bioink‐Nanofiber Constructs: Effect of Rheological Properties of Bioinks on 3D (Bio)Printing and Cells Interaction with Aligned Touch Spun Nanofibers

Fibrous Scaffolds for Muscle Tissue Engineering Based on Touch‐Spun Poly(Ester‐Urethane) Elastomer

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