Enhanced neurite outgrowth of PC-12 cells on graphene-monolayer-coated substrates as biomimetic cues

Lee, Jong Ho; Shin, Yong Cheol; Jin, Oh Seong; Han, Dong‐Wook; Kang, Sang Hoon; Hong, Suck Won; Kim, Jong Man

doi:10.3938/jkps.61.1696

Cited by 13 publications

(11 citation statements)

References 18 publications

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“…To date, the interaction between pristine graphene and peripheral neural cells has been investigated only in two studies (Lee et al, 2012 ; Hong et al, 2014 ), which suggest a positive effect on neurite outgrowth and proliferation when using graphene coated with fetal bovine serum (FBS). However, in both studies bare glass is used as control, thus the effect on the results of FBS coating, which per se is not a traditional coating for neural cells (Sun et al, 2012 ), is not investigated.…”

Section: Introductionmentioning

confidence: 99%

Peripheral Neuron Survival and Outgrowth on Graphene

et al. 2018

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Graphene displays properties that make it appealing for neuroregenerative medicine, yet its interaction with peripheral neurons has been scarcely investigated. Here, we culture on graphene two established models for peripheral neurons: PC12 cells and DRG primary neurons. We perform a nano-resolved analysis of polymeric coatings on graphene and combine optical microscopy and viability assays to assess the material cytocompatibility and influence on differentiation. We find that differentiated PC12 cells display a remarkably increased neurite length on graphene (up to 27%) with respect to controls. Notably, DRG primary neurons survive both on bare and coated graphene. They present dense axonal networks on coated graphene, while they form cell islets characterized by dense axonal bundles on uncoated graphene. These findings indicate that graphene holds potential for nerve tissue regeneration and might pave the road to novel concepts of active nerve conduits.

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Section: Introductionmentioning

confidence: 99%

Peripheral Neuron Survival and Outgrowth on Graphene

et al. 2018

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“…To examine the initial attachment and proliferation of C2C12 skeletal myoblasts on GO-PLGA/RGD nanofiber sheets, a cell counting kit-8 assay (CCK-8 assay, Dojindo, Kumamoto, Japan) was performed following the manufacturer's protocol. The number of viable cells was directly proportional to the metabolic reaction products obtained in the CCK-8 assay 34 , 35 . Briefly, a concentration of 1×10 4 cells/mL was seeded on each sheet, and incubated for 4 h (initial cell attachment) or 1, 3, 5 and 7 days (proliferation) at 37 ºC.…”

Section: Methodsmentioning

confidence: 99%

Ternary Aligned Nanofibers of RGD Peptide-Displaying M13 Bacteriophage/PLGA/Graphene Oxide for Facilitated Myogenesis

et al. 2018

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Recently, there have been tremendous efforts to develop the biofunctional scaffolds by incorporating various biochemical factors. In the present study, we fabricated poly(lactic-co-glycolic acid) (PLGA) nanofiber sheets decorated with graphene oxide (GO) and RGD peptide. The decoration of GO and RGD peptide was readily achieved by using RGD peptide-displaying M13 bacteriophage (RGD-M13 phage) and electrospinning. Furthermore, the aligned GO-decorated PLGA/RGD peptide (GO-PLGA/RGD) ternary nanofiber sheets were prepared by magnetic field-assisted electrospinning, and their potentials as bifunctional scaffolds for facilitating myogenesis were explored. We characterized the physicochemical and mechanical properties of the sheets by scanning electron microscopy, Raman spectroscopy, contact angle measurement, and tensile test. In addition, the C2C12 skeletal myoblasts were cultured on the aligned GO-PLGA/RGD nanofiber sheets, and their cellular behaviors, including initial attachment, proliferation and myogenic differentiation, were evaluated. Our results revealed that the GO-PLGA/RGD nanofiber sheets had suitable physicochemical and mechanical properties for supporting cell growth, and could significantly promote the spontaneous myogenic differentiation of C2C12 skeletal myoblasts. Moreover, it was revealed that the myogenic differentiation was further accelerated on the aligned GO-PLGA/RGD nanofiber sheets due to the synergistic effects of RGD peptide, GO and aligned nanofiber structure. Therefore, , it is suggested that the aligned GO-PLGA/RGD ternary nanofiber sheets are one of the most promising approaches for facilitating myogenesis and promoting skeletal tissue regeneration.

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“…The number of viable cells was directly proportional to the metabolic reaction products obtained in the CCK-8 assay [33, 34]. Briefly, a concentration of 1 × 10 4 cells/ml was seeded on each mat, and incubated for 4 h (cell attachment) or 1, 3, 5 and 7 days (proliferation) at 37°C.…”

Section: Methodsmentioning

confidence: 99%

RGD peptide and graphene oxide co-functionalized PLGA nanofiber scaffolds for vascular tissue engineering

Shin¹,

Kim²,

Kim³

et al. 2017

Regenerative Biomaterials

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In recent years, much research has been suggested and examined for the development of tissue engineering scaffolds to promote cellular behaviors. In our study, RGD peptide and graphene oxide (GO) co-functionalized poly(lactide-co-glycolide, PLGA) (RGD-GO-PLGA) nanofiber mats were fabricated via electrospinning, and their physicochemical and thermal properties were characterized to explore their potential as biofunctional scaffolds for vascular tissue engineering. Scanning electron microscopy images revealed that the RGD-GO-PLGA nanofiber mats were readily fabricated and composed of random-oriented electrospun nanofibers with average diameter of 558 nm. The successful co-functionalization of RGD peptide and GO into the PLGA nanofibers was confirmed by Fourier-transform infrared spectroscopic analysis. Moreover, the surface hydrophilicity of the nanofiber mats was markedly increased by co-functionalizing with RGD peptide and GO. It was found that the mats were thermally stable under the cell culture condition. Furthermore, the initial attachment and proliferation of primarily cultured vascular smooth muscle cells (VSMCs) on the RGD-GO-PLGA nanofiber mats were evaluated. It was revealed that the RGD-GO-PLGA nanofiber mats can effectively promote the growth of VSMCs. In conclusion, our findings suggest that the RGD-GO-PLGA nanofiber mats can be promising candidates for tissue engineering scaffolds effective for the regeneration of vascular smooth muscle.

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Enhanced neurite outgrowth of PC-12 cells on graphene-monolayer-coated substrates as biomimetic cues

Cited by 13 publications

References 18 publications

Peripheral Neuron Survival and Outgrowth on Graphene

Peripheral Neuron Survival and Outgrowth on Graphene

Ternary Aligned Nanofibers of RGD Peptide-Displaying M13 Bacteriophage/PLGA/Graphene Oxide for Facilitated Myogenesis

RGD peptide and graphene oxide co-functionalized PLGA nanofiber scaffolds for vascular tissue engineering

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