Enhanced net mass transfer of HDL cholesteryl esters to Apo B-containing lipoproteins in patients with peripheral vascular disease

Rühling, K.; Zabel-Langhennig, R; Till, U; Thielmann, K

doi:10.1016/0009-8981(89)90062-4

Cited by 12 publications

(1 citation statement)

References 18 publications

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“…Another characteristic feature of HDL from plasma with polydisperse LDL compared with HDL from plasma with monodisperse LDL was a higher cholesteryl ester: triglyceride ratio. By promoting the transfer of cholesteryl esters from HDLs to apo B-containing particles in exchange for a reciprocal transfer of triglycerides in the reverse direction, 1 human CETP has been shown to alter both the LDL pattern 20 -22 and the HDL cholesteryl ester: triglyceride ratio/ 5 Thus, lipid transfers in vivo could affect not only the composition but also the size distribution of lipoprotein particles. This hypothesis was evaluated by incubating several samples of normolipidemic plasma with typical monodisperse and polydisperse LDL patterns at 37°C in the absence of LCAT activity.…”

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confidence: 99%

Influence of plasma cholesteryl ester transfer activity on the LDL and HDL distribution profiles in normolipidemic subjects.

Lagrost¹,

Gandjini²,

Athias³

et al. 1993

Arterioscler Thromb

114

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The relations of cholesteryl ester transfer protein (CETP) activity to the distribution of low density lipoproteins (LDLs) and high density lipoproteins (HDLs) were investigated in fasting plasma samples from 27 normolipidemic subjects. LDL and HDL subtractions were separated by electrophoresis on 20-160 g/L and 40-300 g/L polyacrylamide gradient gels, respectively. Subjects were subdivided into two groups according to their LDL pattern. Monodisperse patterns were characterized by the presence of a single LDL band, whereas polydisperse patterns were characterized by the presence of several LDL bands of different sizes. To investigate the influence of lipid transfers on LDL patterns, total plasma was incubated at 37°C in the absence of lecithin: cholesterol acyltransferase (LCAT) activity. The incubation induced a progressive transformation of polydisperse patterns into monodisperse patterns. Under the same conditions, initially monodisperse patterns remained unchanged. Measurements of the rate of radiolabeled cholesteryl esters transferred from HDL 3 s to very low density lipoproteins (VLDLs) and LDLs revealed that subjects with a monodisperse LDL pattern presented a significantly higher plasma CETP activity than subjects with a polydisperse LDL pattern (301±85%/hr per milliliter versus 216±47%/hr per milliliter, respectively; p<0.02). In addition, when total plasma was incubated for 24 hours at 37°C in the absence of LCAT activity, the relative mass of cholesteryl esters transferred from

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confidence: 99%