Enhanced nerve autograft using stromal vascular fraction

Mesenchymal stem cells and local tacrolimus delivery synergistically enhance neurite extension

“…Low dose irradiation is commonly used to reduce antigenicity by selectively eliminating lymphocytes to suppress immunorejection (Evans et al, 1997; Kaya et al, 2021; Mackinnon et al, 1984 Marmor, 1972; Schaue and McBride 2012; Stearns, 1982) and is argued to damage Schwann cells in a dose‐dependent fashion (Oh et al, 2005). Previous research showed that treatment of autografts, which are naturally covered in Schwann cells, with MSCs resulted in an increase in nerve regeneration (Suganuma et al, 2013; Tada et al, 2021), which was also corroborated in this study. The paracrine properties of MSCs include the secretion of humeral factors resulting in the proliferation and migration of pre‐existing Schwann cells, next to differentiating into additional Schwann cells (Suganuma et al, 2013; Tada et al, 2021).…”

“…Previous research showed that treatment of autografts, which are naturally covered in Schwann cells, with MSCs resulted in an increase in nerve regeneration (Suganuma et al, 2013;Tada et al, 2021), which was also corroborated in this study. The paracrine properties of MSCs include the secretion of humeral factors resulting in the proliferation and migration of pre-existing Schwann cells, next to differentiating into additional Schwann cells (Suganuma et al, 2013;Tada et al, 2021).…”

The Effect of Mesenchymal Stem Cells, Adipose Tissue Derived Stem Cells, and Cellular Stromal Vascular Fraction on the Repair of Acute Anal Sphincter Injury in Rats

“…Compared to ADSC, the preparation of SVF is even simpler, with single protease digestion yielding 2–6 × 10 6 SVF cells (CSVF) per 1 mL of fat with ≥90% cell viability. Many studies have shown that ADSC and CSVF can promote tissue repairs, such as skeletal tissue [ 28 , 29 , 30 , 31 ], muscle tissue [ 32 , 33 , 34 ], nerve damage [ 35 , 36 , 37 , 38 ], and cardiovascular injury [ 39 , 40 , 41 ]. Meanwhile, the tissue source of ADSC and CSVF is widely available, and a large amount of adipose tissue can be obtained for ADSC and CSVF extraction during liposuction, manual liposuction, etc., while the invasive procedure in obtaining bone marrow MSC and muscle-derived stem cells have limited their application in clinic.…”