Enhanced Nanoassembly-Incorporated Antibacterial Composite Materials

Schnaider, Lee; Ghosh, Moumita; Bychenko, Darya; Grigoriants, Irena; Ya'ari, Sarah; Antsel, Tamar Shalev; Matalon, Shlomo; Sarig, Rachel; Brosh, Tamar; Pilo, Raphael; Gazit, Ehud; Adler‐Abramovich, Lihi

doi:10.1021/acsami.9b02839

Cited by 39 publications

(50 citation statements)

References 62 publications

(97 reference statements)

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“…In addition, the coating slightly reduced the adherence of the E. faecalis and S. mutans to the surface. Therefore, the antibacterial properties of the Fmoc-F 5 -Phe structures that were previously demonstrated when incorporated into dental resin [ 74 ] are retained when drop-casted to form a thin antibacterial surface coating. This coating could potentially be used to fabricate dental appliances such as those used in the field of orthodontics and prosthodontics, to reduce the incidence of dental caries, thus improving oral health.…”

Section: Discussionmentioning

confidence: 99%

“…These metallohydrogels have been shown to have a significant antibacterial effect against both Gram-negative ( Escherichia coli ) and Gram-positive ( Staphylococcus aureus ) bacteria in cells and mice by their interaction with the cell walls and membrane, resulting in the detachment of the plasma membrane and leakage of the cytoplasm [ 24 ]. In another study, Nilsson and coworkers designed the Fluorenylmethoxycarbonyl (Fmoc)-pentafluoro-phenylalanine (Fmoc-F 5 -Phe) [ 73 ], which was later studied for its antibacterial properties [ 74 ]. Fmoc-F 5 -Phe nano-assemblies were incorporated into a dental resin-based composite restorative material and demonstrated their biocompatibility along with an inhibitory effect on Streptococcus mutans ( S. mutans ) in solution, targeting the bacterial cell membrane.…”

Section: Introductionmentioning

confidence: 99%

“…Fmoc-F 5 -Phe nano-assemblies were incorporated into a dental resin-based composite restorative material and demonstrated their biocompatibility along with an inhibitory effect on Streptococcus mutans ( S. mutans ) in solution, targeting the bacterial cell membrane. This modified amino acid is comprised of the Fmoc-Phe moiety that induces nano-structure formation and the fluoride moieties, which are utilized for their antibacterial activity [ 74 ]. The crystallographic structure of Fmoc-F 5 -Phe was recently deciphered, leading to an understanding of the molecular interaction of thermodynamically stable structures, including π-π interaction and hydrogen bonding [ 75 ].…”

Section: Introductionmentioning

confidence: 99%

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Surface Modification by Nano-Structures Reduces Viable Bacterial Biofilm in Aerobic and Anaerobic Environments

Ya'ari

Halperin‐Sternfeld

Rosin

et al. 2020

IJMS

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Bacterial biofilm formation on wet surfaces represents a significant problem in medicine and environmental sciences. One of the strategies to prevent or eliminate surface adhesion of organisms is surface modification and coating. However, the current coating technologies possess several drawbacks, including limited durability, low biocompatibility and high cost. Here, we present a simple antibacterial modification of titanium, mica and glass surfaces using self-assembling nano-structures. We have designed two different nano-structure coatings composed of fluorinated phenylalanine via the drop-cast coating technique. We investigated and characterized the modified surfaces by scanning electron microscopy, X-ray diffraction and wettability analyses. Exploiting the antimicrobial property of the nano-structures, we successfully hindered the viability of Streptococcus mutans and Enterococcus faecalis on the coated surfaces in both aerobic and anaerobic conditions. Notably, we found lower bacteria adherence to the coated surfaces and a reduction of 86–99% in the total metabolic activity of the bacteria. Our results emphasize the interplay between self-assembly and antimicrobial activity of small self-assembling molecules, thus highlighting a new approach of biofilm control for implementation in biomedicine and other fields.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Surface Modification by Nano-Structures Reduces Viable Bacterial Biofilm in Aerobic and Anaerobic Environments

Ya'ari

Halperin‐Sternfeld

Rosin

et al. 2020

IJMS

Self Cite

View full text Add to dashboard Cite

show abstract

“…The use of antimicrobial peptides in composite restorations has been reported in one study where Fmoc-pentafluoro- L -phenylalanine-oh (fmoc-f 5 -phe) was functionalized and incorporated into a commercial resin composite at different concentrations: 0.25, 0.5, 1, and 2 w/w % [ 95 ]. Fmoc-F 5 -Phe is a self-assembled antibacterial and anti-inflammatory peptide.…”

Section: Contact-killing Materials As a Strategy In Resin-based Rementioning

confidence: 99%

Emerging Contact-Killing Antibacterial Strategies for Developing Anti-Biofilm Dental Polymeric Restorative Materials

et al. 2020

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Polymeric materials are the first choice for restoring tooth cavities, bonding tooth-colored fillings, sealing root canal systems, and many other dental restorative applications. However, polymeric materials are highly susceptible to bacterial attachment and colonization, leading to dental diseases. Many approaches have been investigated to minimize the formation of biofilms over polymeric restorative materials and at the tooth/material interfaces. Among them, contact-killing compounds have shown promising results to inhibit dental biofilms. Contact-killing compounds can be immobilized within the polymer structure, delivering a long-lasting effect with no leaching or release, thus providing advantages compared to release-based materials. This review discusses cutting-edge research on the development of contact-killing compounds in dental restorative materials to target oral pathogens. Contact-killing compounds in resin composite restorations, dental adhesives, root canal sealers, denture-based materials, and crown cements have all demonstrated promising antibacterial properties. Contact-killing restorative materials have been found to effectively inhibit the growth and activities of several oral pathogens related to dental caries, periodontal diseases, endodontic, and fungal infections. Further laboratory optimization and clinical trials using translational models are needed to confirm the clinical applicability of this new generation of contact-killing dental restorative materials.

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“…We recently found that self-assembly of minimal amyloid models augments their antibacterial activity (46). Furthermore, impressive intrinsic antibacterial activity (47)(48)(49)(50) has been detected in several studies using aromatic-amino-acid-based self-assembling building blocks (47)(48)(49)(50). Whether self-assembly has a role in antifungal peptide activity is not well established.…”

mentioning

confidence: 99%

Peptide Self-Assembly Is Linked to Antibacterial, but Not Antifungal, Activity of Histatin 5 Derivatives

Schnaider

Rosenberg

Kreiser

et al. 2020

mSphere

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The rise of multidrug-resistant pathogens has awakened interest in new drug candidates such as antimicrobial peptides and their derivatives. Recent work suggests that some antimicrobial peptides have the ability to self-assemble into ordered amyloid-like nanostructures which facilitate their antibacterial activity. Here, we evaluate a histatin-based antimicrobial peptide, and its self-assembling derivative, in the interplay between self-assembly, membrane interactions, and antibacterial and antifungal activities. We demonstrate substantial membrane targeting by both peptides, as well as mechanistic insights into this mode of action, which correlates to their antifungal activity and is not affected by their self-assembling state. The ability to self-assemble does, however, significantly affect peptide antibacterial activity against both Gram-negative and Gram-positive bacteria. These results are surprising and hint at important distinctions between antifungal and antibacterial peptide activities in prokaryotes and eukaryotic microbes. IMPORTANCE Antimicrobial peptides are important modulators of host defense against bacterial, fungal, and viral pathogens in humans and other multicellular organisms. Two converging paradigms point to a link between antimicrobial peptides that self-assemble into amyloid-like nanoassemblies and classical amyloidogenic peptides that often have potent broad-spectrum antimicrobial activity, suggesting that antimicrobial and amyloidogenic peptides may represent two sides of the same coin. Here, we asked if the ability of an antifungal peptide to self-assemble affects its antifungal or antibacterial activity. We found that modifications of classical antifungal peptide derivative allowed it to self-assemble and did not alter its antifungal activity, and yet self-assembly substantially increased the antibacterial activity of the peptide. These results support the idea that peptide self-assembly can enhance antibacterial activities and emphasize a distinction between the action of antifungal peptides and that of antibacterial peptides. Accordingly, we suggest that the possible generality of this distinction should be widely tested.

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Enhanced Nanoassembly-Incorporated Antibacterial Composite Materials

Cited by 39 publications

References 62 publications

Surface Modification by Nano-Structures Reduces Viable Bacterial Biofilm in Aerobic and Anaerobic Environments

Surface Modification by Nano-Structures Reduces Viable Bacterial Biofilm in Aerobic and Anaerobic Environments

Emerging Contact-Killing Antibacterial Strategies for Developing Anti-Biofilm Dental Polymeric Restorative Materials

Peptide Self-Assembly Is Linked to Antibacterial, but Not Antifungal, Activity of Histatin 5 Derivatives

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