Enhanced monoterpene emission in transgenic orange mint (Mentha × piperita f. citrata) overexpressing a tobacco lipid transfer protein (NtLTP1)

Hwang, Hwan-Su; Adhikari, Prakash Babu; Jo, Hye-Jeong; Han, Jung Yeon; Choi, Yong Eui

doi:10.1007/s00425-020-03447-6

Cited by 14 publications

(7 citation statements)

References 39 publications

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“…An increase in this enzyme can lead to myocardial necrosis, liver damage, skeletal muscle damage, or pancreatitis [ 7 ]. Linalool (40.43%) was the dominant constituent of the EO from M. × piperita f. citrata ( M3 ), which is consistent with the literature [ 14 ].…”

Section: Resultssupporting

confidence: 89%

Headspace Solid-Phase Micro-Extraction Versus Hydrodistillation of Volatile Compounds from Leaves of Cultivated Mentha Taxa: Markers of Safe Chemotypes

et al. 2022

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Various mint taxa are widely cultivated and are used not only for medicinal purposes but also in cosmetic and industrial applications. The development of new varieties or cultivars of mint generates difficulties in their correct identification and safe use. Volatile organic compounds (VOCs) from the leaves of seven different taxa of the genus Mentha obtained by hydrodistillation (HD) and headspace solid-phase microextraction (HS-SPME) were analyzed using gas chromatography–mass spectrometry (GC-MS). Principal component analysis (PCA) was also performed. Comparative GC-MS analysis of the obtained extracts showed similarity in the major compounds. PCA data allowed the separation of two groups of chemotypes among the analyzed mints, characterized by the abundance of piperitenone oxide and carvone. Two out of seven analyzed taxa were not previously examined for VOC profile, one was examined only for patent application purposes, and six out of seven were investigated for the first time using the HS-SPME technique. The presented analysis provides new data on the abundance and qualitative characterization of VOCs in the studied mint plants and on the safety of their use, related to the possibility of the presence of potentially toxic components. HS-SPME is a valuable method to extend the characterization of the VOC profile obtained by hydrodistillation.

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Section: Resultssupporting

confidence: 89%

Headspace Solid-Phase Micro-Extraction Versus Hydrodistillation of Volatile Compounds from Leaves of Cultivated Mentha Taxa: Markers of Safe Chemotypes

et al. 2022

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“…They share 58/69% identity/similarity with each other (Supplementary Table 2) and cluster together with other functional nsLTPs (Fig. 1b) including NtLTP1, which is involved in lipid secretion in tobacco and monoterpenes transport upon its overexpression in mint 13,26 , and AaLTP3 that is responsible for sesquiterpene lactone secretion 21 . PhnsLTP3 encodes a protein of 115 amino acids with calculated molecular mass for mature protein of 9.65 kDa that shares 40/60% and 41/60% identity/similarity with PhnsLTP1 and PhnsLTP2, respectively (Supplementary Tables 1 and 2).…”

Section: Resultsmentioning

confidence: 95%

“…It has been shown that nsLTPs are involved in delivering wax components to the cuticle 11,12,20 , as well as in the secretion of monoterpenes 26 , sesquiterpenes 14,21 and diterpenes 13 from the secretory head cells to the subcuticular storage cavities of glandular trichomes. More than half a decade ago, it was proposed that nsLTPs could also facilitate VOC transport across the hydrophilic cell wall en route to the atmosphere 6 .…”

Section: Discussionmentioning

confidence: 99%

Emission of floral volatiles is facilitated by cell-wall non-specific lipid transfer proteins

Liao

Maoz

Shih

et al. 2022

Preprint

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For volatile organic compounds (VOCs) to be released from the plant cell into the atmosphere, they have to cross the plasma membrane, the cell wall, and the cuticle. However, how these hydrophobic compounds cross the hydrophilic cell wall is largely unknown. Using biochemical and reverse-genetic approaches combined with mathematical modeling, we show that cell-wall localized non-specific lipid transfer proteins (nsLTPs) facilitate VOC emission. Out of three highly expressed nsLTPs in petunia petals, which emit high levels of phenylpropanoid/benzenoid compounds, only PhnsLTP3 contributes to the VOC export across the cell wall to the cuticle. A decrease in PhnsLTP3 expression reduces volatile emission and leads to VOC redistribution with less VOCs reaching the cuticle without affecting their total pools. This intracellular build-up of VOCs lowers their biosynthesis by feedback downregulation of phenylalanine precursor supply to prevent self-intoxication. Overall, these results demonstrate that nsLTPs are intrinsic members of the VOC emission network, which facilitate VOC diffusion across the cell wall.

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“…The above examples focus on the targetting of specific genes in the monoterpenoid biosynthesis pathway but there have been efforts to increase lipid flux in the trichomes. Hwang et al (2020) transformed a lipid transfer protein from N. tabacum (NtLTP1) into M. × piperita f. citrate (Orange mint). These NtLTP1 overexpressing lines had increased trichome head diameters compared to the WT controls ( Hwang et al, 2020 ).…”

Section: Engineering Monoterpenoid Biosynthesismentioning

confidence: 99%

“… Hwang et al (2020) transformed a lipid transfer protein from N. tabacum (NtLTP1) into M. × piperita f. citrate (Orange mint). These NtLTP1 overexpressing lines had increased trichome head diameters compared to the WT controls ( Hwang et al, 2020 ). They also saw large increases in monoterpenoid production with limonene levels increasing 1.6 fold and 3-pinanone levels increasing over 22 fold.…”

Section: Engineering Monoterpenoid Biosynthesismentioning

confidence: 99%

Genetic Manipulation of Biosynthetic Pathways in Mint

et al. 2022

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In recent years, the study of aromatic plants has seen an increase, with great interest from industrial, academic, and pharmaceutical industries. Among plants attracting increased attention are the Mentha spp. (mint), members of the Lamiaceae family. Mint essential oils comprise a diverse class of molecules known as terpenoids/isoprenoids, organic chemicals that are among the most diverse class of naturally plant derived compounds. The terpenoid profile of several Mentha spp. is dominated by menthol, a cyclic monoterpene with some remarkable biological properties that make it useful in the pharmaceutical, medical, cosmetic, and cleaning product industries. As the global market for Mentha essential oils increases, the desire to improve oil composition and yield follows. The monoterpenoid biosynthesis pathway is well characterised so metabolic engineering attempts have been made to facilitate this improvement. This review focuses on the Mentha spp. and attempts at altering the carbon flux through the biosynthetic pathways to increase the yield and enhance the composition of the essential oil. This includes manipulation of endogenous and heterologous biosynthetic enzymes through overexpression and RNAi suppression. Genes involved in the MEP pathway, the menthol and carvone biosynthetic pathways and transcription factors known to affect secondary metabolism will be discussed along with non-metabolic engineering approaches including environmental factors and the use of plant growth regulators.

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Enhanced monoterpene emission in transgenic orange mint (Mentha × piperita f. citrata) overexpressing a tobacco lipid transfer protein (NtLTP1)

Cited by 14 publications

References 39 publications

Headspace Solid-Phase Micro-Extraction Versus Hydrodistillation of Volatile Compounds from Leaves of Cultivated Mentha Taxa: Markers of Safe Chemotypes

Headspace Solid-Phase Micro-Extraction Versus Hydrodistillation of Volatile Compounds from Leaves of Cultivated Mentha Taxa: Markers of Safe Chemotypes

Emission of floral volatiles is facilitated by cell-wall non-specific lipid transfer proteins

Genetic Manipulation of Biosynthetic Pathways in Mint

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