Enhanced microfiltration of γ-globulin solution upon treatment of NaCl addition and/or DNase digestion

Higuchi, Akon; Nemoto, Masashi; Koyama, Hideaki; Hirano, Kyousuke; Yoon, Boo Ok; Hara, Mariko; Yokogi, Masanobu; Manabe, Sei-ichi

doi:10.1016/s0376-7388(02)00417-9

Cited by 11 publications

(4 citation statements)

References 24 publications

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“…As such, regenerated cellulose gels in water-swollen state, including wet cellophane films for dialysis [2][3][4] and liquid chromatography packings [5][6][7][8][9], have been used as separation media exploiting the chemical inertness and mechanical stability of cellulose. While these cellulose hydrogels have nanometer-sized pores through control of preparation conditions, direct drying of these gels usually results in total collapse of porosity; i.e., the strong surface tension of leaving water [10] causes cohesion of cellulose molecules forming a tight mass.…”

Section: Introductionmentioning

confidence: 99%

Nanofibrillar cellulose aerogels

Hao

Nishiyama

Wada

et al. 2004

Colloids and Surfaces A: Physicochemical and Engineering Aspect

325

249

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Section: Introductionmentioning

confidence: 99%

Nanofibrillar cellulose aerogels

Hao

Nishiyama

Wada

et al. 2004

Colloids and Surfaces A: Physicochemical and Engineering Aspect

325

249

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“…Higuchi et al (2001Higuchi et al ( , 2002 observed a large increase in flux and capacity for the Planova virus filters when operated at high NaCl concentrations or after treatment of the feed with DNase. The authors attributed these improvements to dissociation of large complexes formed between trace amounts of DNA and the g-globulins.…”

Section: Introductionmentioning

confidence: 99%

Effect of solution pH on protein transmission and membrane capacity during virus filtration

Bakhshayeshi

Zydney

2007

Biotech & Bioengineering

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Although virus filtration is now an integral part of the overall viral clearance strategy for the purification of many commercial therapeutic proteins, there is currently little understanding of the factors controlling the performance of the virus filters. The objective of this study was to examine the effects of solution pH on protein transmission and capacity during virus filtration. Data were obtained using bovine serum albumin as a model protein with Viresolve 180 membranes oriented with both the skin-side up and skin-side down. Membranes were also characterized using dextran sieving measurements both before and after protein filtration. Membrane capacity and protein yield were minimal at the protein isoelectric point, which was due to the greater degree of concentration polarization associated with the smaller protein diffusion coefficient at this pH. In contrast, the actual protein sieving coefficient was maximum at the protein isoelectric point due to the absence of any strong electrostatic exclusion under these conditions. The yield and capacity were both significantly greater when the membrane was oriented with the skin-side down. These results provide important insights into the effects of solution conditions on the performance of virus filtration membranes for protein purification.

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“…The capacity in the absence of salt was only 10 L/m 2 as a result of the rapid fouling under these conditions. The capacity was significantly larger in the presence of salt (8) or upon treatment with DNase (9), which the authors attributed to the dissociation of large complexes that formed between the γ‐globulin and trace amounts of DNA present in the feed solution. These complexes were thought to block the pores in the multilayer structure of the Planova membranes.…”

Section: Introductionmentioning

confidence: 99%

Protein Fouling of Virus Filtration Membranes: Effects of Membrane Orientation and Operating Conditions

2006

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The capacity of virus filters used in the purification of therapeutic proteins is determined by the rate and extent of membrane fouling. Current virus filtration membranes have a complex multilayer structure that can be used with either the skin-side up or with the skin-side facing away from the feed, but there is currently no quantitative understanding of the effects of membrane orientation or operating conditions on the filtration performance. Experiments were performed using Millipore's Viresolve 180 membrane under both constant pressure and constant flux operation with sulfhydryl-modified BSA used as a model protein. The capacity with the skin-side up was greater during operation with constant flux and at low transmembrane pressures, with the flux decline or pressure rise due primarily to osmotic pressure effects. In contrast, data obtained with the skin-side down showed a slower, steady increase in total resistance with the cumulative filtrate volume, with minimal contribution from osmotic pressure. The capacity with the skin-side down was significantly greater than that with the skin-side up, reflecting the different fouling mechanisms in the different membrane orientations. These results provide important insights for the design and operation of virus filtration membranes.

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Enhanced microfiltration of γ-globulin solution upon treatment of NaCl addition and/or DNase digestion

Cited by 11 publications

References 24 publications

Nanofibrillar cellulose aerogels

Nanofibrillar cellulose aerogels

Effect of solution pH on protein transmission and membrane capacity during virus filtration

Protein Fouling of Virus Filtration Membranes: Effects of Membrane Orientation and Operating Conditions

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