Enhanced microalgal lipid production for biofuel using different strategies including genetic modification of microalgae: A review

Khoo, Kuan Shiong; Ahmad, Imran; Chew, Kit Wayne; Iwamoto, Koji; Bhatnagar, Amit; Show, Pau Loke

doi:10.1016/j.pecs.2023.101071

Cited by 100 publications

(31 citation statements)

References 342 publications

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“…The lipids in microalgae are enriched in PUFAs such as DHA, EPA, ALA, and so on, and free from cholesterol which is favorable in diseases like atherosclerosis, obesity, and blood pressure [38]. Moreover, higher lipid productivity in microalgae is a desirable factor for biofuel and biodiesel production [39]. As reported in this study BGLRS showed a good amount of lipid content and could be a good potential source for bioenergy applications.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, carbohydrates can retain chemical structure to a great extent under drying methods as compared with other biomolecules. Carbohydrates present in microalgae, for example, in Spirulina , are cellulose and sugar‐free which confers them the property of easy digestibility and is ideal for diabetic and obese patients [39]. Additionally, microalgal‐derived carbohydrates such as pectic‐oligosaccharides, fructose‐oligosaccharides, xylo‐oligosaccharide, and so on, have prebiotic properties and stimulate specific gut microbes like Bifidobacteria and Lactobacillus [40].…”

Section: Discussionmentioning

confidence: 99%

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Characterization of halotolerant microalga isolated from waterlogged habitats: Deciphering the biochemical profiling and unraveling the molecular identity

Sharma,

Kaur,

Gupta Phutela

et al. 2024

J Basic Microbiol

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The primary objective of this study was to comprehensively explore the biochemical profile of the novel halotolerant microalgae strain, biogas laboratory scenedesmus (BGLRS), previously isolated from waterlogged regions in the southwest zone of Punjab, India. To achieve this, three distinct drying methods viz. freeze‐drying, oven‐drying, and shade‐drying were employed and biochemical composition and antioxidant analyses on the microalgal biomass were conducted. Utilizing advanced analytical techniques, including high‐performance liquid chromatography (HPLC), inductively coupled plasma‐atomic emission spectroscopy (ICP‐AES), and gas chromatography‐mass spectroscopy (GC‐MS) on freeze‐dried biomass, its carbohydrate profile, micronutrient composition, and presence of bioactive compounds with potential therapeutic and nutraceutical significance were sought to unravel. Among the drying methods evaluated, freeze‐drying exhibited the most promising experimental results, prompting its selection for further investigation. Notably, ICP‐AES unveiled elevated concentrations of essential elements such as calcium, iron, magnesium, and phosphorus in BGLRS, with negligible traces of heavy metals, underscoring its safety for human consumption. GC‐MS analysis further divulged the existence of numerous biologically active compounds, indicating potential applications in medical and nutraceutical fields. Through molecular identification using sequencing of the internal transcribed spacer (ITS) region, a close taxonomic resemblance between BGLRS and Scenedesmus sp. MKB was established, solidifying its unique position within the microalgal taxonomy. The deposition of ITS sequences into the NCBI GenBank, obtaining accession number MN796425, attests to the rigor and transparency of this research. Overall, these findings strongly suggest that microalgae BGLRS possesses high‐quality biochemical attributes of significant therapeutic and nutraceutical importance.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Characterization of halotolerant microalga isolated from waterlogged habitats: Deciphering the biochemical profiling and unraveling the molecular identity

Sharma,

Kaur,

Gupta Phutela

et al. 2024

J Basic Microbiol

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“…According to substrate preferences of diacylglycerol acyltransferases (DGATs) toward fatty acids with different degrees of desaturation, fatty acid composition of TAG could be tuned by modulating the expression of different DGATs in Nannochloropsis oceanica . Recently, transcription factor (TF) modification for increasing lipid content has become increasingly popular in microalgae, due to their ability to regulate multiple genes simultaneously while not compromising biomass. , Nonetheless, limited transcription factors have been characterized to modulate fatty acid desaturation in microalgae, and the transcriptionally regulatory mechanisms remained largely unknown.…”

Section: Introductionmentioning

confidence: 99%

“…13 Recently, transcription factor (TF) modification for increasing lipid content has become increasingly popular in microalgae, due to their ability to regulate multiple genes simultaneously while not compromising biomass. 2,14 Nonetheless, limited transcription factors have been characterized to modulate fatty acid desaturation in microalgae, and the transcriptionally regulatory mechanisms remained largely unknown.…”

Section: Introductionmentioning

confidence: 99%

“…This characteristic holds tremendous potential for the production of biofuels, food, feed, and food additives . The properties and practical applications of microalgal products are primarily governed by fatty acid desaturation, with monounsaturated fatty acids (MUFAs) such as oleic acid (C18:1) being preferred for biodiesel production. , In contrast, polyunsaturated fatty acids (PUFAs) like linolenic acid (C18:3), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA) offer health benefits and aid in disease prevention . In microalgae, fatty acids are de novo synthesized in the chloroplast, and PUFAs are biosynthesized using the fatty acid desaturation and elongation pathways .…”

Section: Introductionmentioning

confidence: 99%

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Non-Tandem CCCH-Type Zinc-Finger Protein CpZF_CCCH1 Improves Fatty Acid Desaturation and Stress Tolerance in Chlamydomonas reinhardtii

Wang,

Li,

Zhang

et al. 2023

J. Agric. Food Chem.

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The properties and nutritional value of microalgal bioproducts depend significantly on fatty acid desaturation, which is generally modulated by manipulating the culture conditions or associated gene expressions. Here, we investigated the role of CpZF_CCCH1, a non-tandem CCCH-type zinc-finger (non-TZF) protein, in elevating polyunsaturated fatty acid (PUFA) content (11.00−16.36%) in Chlamydomonas reinhardtii. Through lipidomic and flow cytometry analyses, we observed reduced triacylglycerol accumulation (7.01−21.15%) and elevated levels of membrane lipids containing PUFAs (7.81−46.18%) in C. reinhardtii overexpressing CpZF_CCCH1. Additionally, overexpression of nucleus-located CpZF_CCCH1 downregulated genes associated with triacylglycerol assembly and lipid turnover from 2.00-to 2.90-fold, likely by binding to GCN4 motif and promoter of 3phosphate-glycerol acyltransferase. Furthermore, overexpression of CpZF_CCCH1 alleviated reactive oxygen species levels by 59.28−73.26% and enhanced stress tolerance under adverse conditions. These findings expanded the roles of non-TZF proteins in lipid metabolism, opening new avenues for metabolic engineering to enhance the nutritional value and stress tolerance of microalgae and agricultural crops.

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Utilizing fungal biodegradation for valorisation of lignocellulosic waste biomass and its diverse applications

Mehta,

Chelike

2024

Applied Research

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Myco degradation is an effective technique for breaking down waste plant substances made of lignin, cellulose, and hemicellulose, which are collectively known as lignocellulose. This abundant organic material is found throughout the world. Due to its recalcitrant nature, lignocellulose poses a challenge for efficient conversion into biofuels, biochemicals, and other valuable products. Myco degradation, which involves the use of fungi to degrade lignocellulosic materials, offers a sustainable and cost‐efficient resolution to this challenge. This review provides an overview of the mechanisms and applications of myco degradation for lignocellulosic biomass degradation. The review discusses the various types of fungi involved in lignocellulose degradation, their enzymatic systems, and the factors that influences their performance. Furthermore, the potential applications of myco degradation products, such as biofuels, enzymes, and bioplastics, are reviewed. It also highlights the implications of myco degradation for waste management and sustainable development. Overall, myco degradation represents a promising technology for the efficient deprivation of lignocellulosic waste biomass, and further research in this field holds great potential for the sustainable creation of bio‐based products.This article is protected by copyright. All rights reserved.

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Enhanced microalgal lipid production for biofuel using different strategies including genetic modification of microalgae: A review

Cited by 100 publications

References 342 publications

Characterization of halotolerant microalga isolated from waterlogged habitats: Deciphering the biochemical profiling and unraveling the molecular identity

Characterization of halotolerant microalga isolated from waterlogged habitats: Deciphering the biochemical profiling and unraveling the molecular identity

Non-Tandem CCCH-Type Zinc-Finger Protein CpZF_CCCH1 Improves Fatty Acid Desaturation and Stress Tolerance in Chlamydomonas reinhardtii

Utilizing fungal biodegradation for valorisation of lignocellulosic waste biomass and its diverse applications

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